Exploration of Metabolic and Endocrine Function in the Mouse

Champy, Marie‐France; Argmann, Carmen; Chambon, Pierre; Auwerx, Johan

doi:10.1002/9783527611942.ch5

Cited by 3 publications

(2 citation statements)

References 41 publications

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“…The EchoMRI 3-in-1 instrument (EchoMRI LLC, Houston, TX) is a quantitative nuclear magnetic resonance (qNMR) imaging system for whole body composition analysis of unaesthesized small animals [119, 120], and qNMR body composition analysis with EchoMRI instrumentation has been proposed to be “gold standard” methodology for metabolic studies in the mouse [121]. Following calibration, each mouse was put in a holder and placed into the EchoMRI chamber and lean mass, fat mass and water mass was calculated.…”

Section: Methodsmentioning

confidence: 99%

ImAge: an imaging approach to quantitate aging and rejuvenation

Rodriguez¹,

Fiengo²,

Alvarez-Kuglen³

et al. 2022

Preprint

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Predictive biomarkers of functional or biological age are key for evaluating interventions aimed at increas-ing healthspan and / or lifespan in humans. Currently, cardiovascular performance, blood analytes, frailty indices (e.g. gait speed), and DNA methylation clocks are used to provide such estimates; each technique has its own challenges and limitations. We have developed a novel approach, microscopic imaging of bio-logical age (miBioAge), which computes multiparametric signatures based on the patterns of epigenetic landscape in single nuclei. We demonstrated that such miBioAge readouts can robustly distinguish young and old cells from multiple tissues, reveal aging progression in peripheral blood (e.g. CD3+ T cells), and reflect changes of epigenetic signatures consistent with expected slowdown or acceleration of biological age in chronologically identical mice treated with caloric restriction or chemotherapy. Because miBioAge readouts are computed from individual samples without applying linear regression, we posit that this novel biomarker may provide personalized assessment of functional aging.

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Section: Methodsmentioning

confidence: 99%

ImAge: an imaging approach to quantitate aging and rejuvenation

Rodriguez¹,

Fiengo²,

Alvarez-Kuglen³

et al. 2022

Preprint

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“…EchoMRI testing. The EchoMRI 3-in-1 instrument (EchoMRI LLC, Houston, TX) is a quantitative nuclear magnetic resonance (qNMR) imaging system for whole body composition analysis of unaesthesized small animals 143,144 , and qNMR body composition analysis with EchoMRI instrumentation has been proposed to be "gold standard" methodology for metabolic studies in the mouse 145 . Following calibration, each mouse was put in a holder and placed into the EchoMRI chamber and lean mass, fat mass and water mass was calculated.…”

Section: Micementioning

confidence: 99%

Imaging-based chromatin and epigenetic age, ImAge, quantitates aging and rejuvenation.

terskikh,

Alvarez-Kuglen,

Rodriguez

et al. 2023

Preprint

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Biomarkers of biological age that predict the risk of disease and expected lifespan better than chronologi-cal age are key to efficient and cost-effective healthcare1–3. To advance a personalized approach to healthcare, such biomarkers must reliably and accurately capture individual biology, predict biological age, and provide scalable and cost-effective measurements. We developed a novel approach – image-based chromatin and epigenetic age (ImAge) that captures intrinsic progressions of biological age, which readily emerge as principal changes in the spatial organization of chromatin and epigenetic marks in single nuclei without regression on chronological age. ImAge captured the expected acceleration or deceleration of bio-logical age in mice treated with chemotherapy or following a caloric restriction regimen, respectively. Im-Age from chronologically identical mice inversely correlated with their locomotor activity (greater activity for younger ImAge), consistent with the widely accepted role of locomotion as an aging biomarker across species. Finally, we demonstrated that ImAge is reduced following transient expression of OSKM cassette in the liver and skeletal muscles and reveals heterogeneity of in vivo reprogramming. We propose that Im-Age represents the first-in-class imaging-based biomarker of aging with single-cell resolution.

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