Exploration of molecular targets and mechanisms of Chinese medicinal formula Acacia Catechu ‐Scutellariae Radix in the treatment of <scp>COVID</scp>‐19 by a systems pharmacology strategy

Tian, Feng; Zhang, Meng; Xu, Qian; Fan, Song; Wang, Libin; Gai, Shouchang; Tang, Haifeng; Wang, Siwang; Zhou, Lei; Li, Hua

doi:10.1002/ptr.7554

Cited by 11 publications

(6 citation statements)

References 82 publications

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“…Therefore, natural compounds in herbs seem to be rich sources for screening potential drugs against coronaviruses. As expected, several herbal medicines as well as isolated compounds in herbs have been reported to possess inhibitory activities against SARS-CoV-2 in the past few years [ 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 ]. Although herbal medicines and their isolated compounds are not as effective as chemical antiviral drugs, they are empirically safe for the human body, particularly after long-term consumption.…”

Section: Introductionmentioning

confidence: 62%

Strictinin, a Major Ingredient in Yunnan Kucha Tea Possessing Inhibitory Activity on the Infection of Mouse Hepatitis Virus to Mouse L Cells

Hsu

Tzen

2023

Molecules

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Theacrine and strictinin of Yunnan Kucha tea prepared from a mutant variety of wild Pu’er tea plants were two major ingredients responsible for the anti-influenza activity. As the COVID-19 outbreak is still lurking, developing safe and cost-effective therapeutics is an urgent need. This study aimed to evaluate the effects of these tea compounds on the infection of mouse hepatitis virus (MHV), a β-coronavirus serving as a surrogate for SARS-CoV. Treatment with strictinin (100 μM), but not theacrine, completely eliminated MHV infection, as indicated by a pronounced reduction in plaque formation, nucleocapsid protein expression, and progeny production of MHV. Subsequently, a time-of-drug addition protocol, including pre-, co-, or post-treatment, was exploited to further evaluate the possible mechanism of antiviral activity mediated by strictinin, and remdesivir, a potential drug for the treatment of SARS-CoV-2, was used as a positive control against MHV infection. The results showed that all three treatments of remdesivir (20 μM) completely blocked MHV infection. In contrast, no significant effect on MHV infection was observed when cells were pre-treated with strictinin (100 μM) prior to infection, while significant inhibition of MHV infection was observed when strictinin was introduced upon viral adsorption (co-treatment) and after viral entry (post-treatment). Of note, as compared with the co-treatment group, the inhibitory effect of strictinin was more striking in the post-treatment group. These results indicate that strictinin suppresses MHV infection by multiple mechanisms; it possibly interferes with viral entry and also critical step(s) of viral infection. Evidently, strictinin significantly inhibited MHV infection and might be a suitable ingredient for protection against coronavirus infection.

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Section: Introductionmentioning

confidence: 62%

Strictinin, a Major Ingredient in Yunnan Kucha Tea Possessing Inhibitory Activity on the Infection of Mouse Hepatitis Virus to Mouse L Cells

Hsu

Tzen

2023

Molecules

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“…Further research revealed that seven key active components (chrysin, baicalein, wogonin, apigenin, isoliquiritigenin, isoliquiritin apioside, and Glycyrrhetinic acid) were regarded to be effective on COVID-19, and twelve targets (MAPK3, IL6, JUN, IL2, STAT3, AKT1, TNF, STAT1, EGFR, HSP90AA1, VEGFA RELA) were screened to be effective on COVID-19. Studies have shown that baicalein and apigenin can inhibit the 3C-like protease of SARS-CoV-2 [ 26 ]. Glycyrrhetinic acid has been shown to have anti-COVID-19 activity [ 27 ].…”

Section: Resultsmentioning

confidence: 99%

Systematically uncovering the absorbed effective substances of Radix Scutellaria-licorice drug pair in rat plasma against COVID-19 using a combined UHPLC-Q-TOF-MS analysis and target network pharmacology

Wen,

Xie,

Gao

et al. 2023

PLoS ONE

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Radix Scutellaria-Licorice drug pair (RSLDP), a frequently used herbal pair with the effect of clearing heat and detoxifying, is the commonly employed drug pair in TCM prescriptions for the treatment of COVID-19. Until now, the metabolism feature and anti-COVID-19 mechanism of RSLDP have not been fully elucidated. In this study, a sensitive and rapid method was developed for the separation and identification of the absorbed constituents of RSLDP in the rat plasma by UHPLC-QTOF-MS. Additionally, we optimized the conventional methodologies of network pharmacology and proposed a new concept called target network pharmacology (T-NP). It used the absorbed constituents and the corresponding targets to generate a compound-target network, and compared to conventional network pharmacology, it could reduce false-positive results. A total of 85 absorbed constituents were identified or tentatively characterized in dosed plasma, including 32 components in the group of Radix Scutellaria, 27 components in the group of Licorice, and 65 components in the group of RSLDP. The results showed that the compatibility of Radix Scutellaria and Licorice increased the number of components in vivo. We found that 106 potential targets among the 61 active compounds in RSLDP were related to COVID-19. And 12 targets (STAT3, AKT1, EGFR, HSP9AA1, MAPK3, JUN, IL6, VEGFA, TNF, IL2, RELA, and STAT1) could be core targets for RSLDP in treating COVID-19. Results from these targets indicate that RSLDP treatment of COVID-19 mainly involves response to chemical stress, response to oxygenates, positive regulation of cytokines, PI3K-Akt signaling pathway, AGE-RAGE signaling pathway for diabetic complications, virus-related pathways such as novel coronavirus and human cytomegalovirus infection, inflammatory immune-related pathways, and so on. The metabolism feature of RSLDP in vivo was systematically uncovered. The combined use of the T-NP method could discover potential drug targets and disclose the biological processes of RSLDP, which will clarify the potential mechanisms of RSLDP in the treatment of COVID-19.

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“…These medicines are used in various health problems e.g. treatment of chronic liver diseases, pulmonary infection therapy, coronary artery disease treatment and more [98,99,100,101]. Fisetin has so far been poorly studied, however multiple therapeutic effects were postulated.…”

Section: Fisetinmentioning

confidence: 99%

Therapeutic potential of flavonoids used in traditional Chinese medicine – a comparative study of galangin, kaempferol, chrysin and fisetin

Sokal,

Stocerz,

Olczyk

et al. 2024

Ann. Acad. Med. Siles.

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Ziołolecznictwo stanowi jedną z głównych gałęzi tradycyjnej medycyny chińskiej (traditional Chinese medicine – TCM). W pracy skupiono się na aktywności biologicznej wybranych flawonoidów, a także konkretnych przykładach wpływu tych substancji na różne układy organizmu. Flawonoidy to grupa związków chemicznych zawartych w surowcach roślinnych, miodzie, propolisie czy grzybach stosowanych w TCM. Chryzyna, galangina, kemferol i fisetyna to przykłady flawonoidów wykazujących m.in. właściwości przeciwutleniające, przeciwzapalne czy przeciwbakteryjne. Właściwości te są przedmiotem wielu badań naukowych, mających na celu zbadanie ich potencjalnego działania terapeutycznego.

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Exploration of molecular targets and mechanisms of Chinese medicinal formula Acacia Catechu ‐Scutellariae Radix in the treatment of COVID‐19 by a systems pharmacology strategy

Cited by 11 publications

References 82 publications

Strictinin, a Major Ingredient in Yunnan Kucha Tea Possessing Inhibitory Activity on the Infection of Mouse Hepatitis Virus to Mouse L Cells

Strictinin, a Major Ingredient in Yunnan Kucha Tea Possessing Inhibitory Activity on the Infection of Mouse Hepatitis Virus to Mouse L Cells

Systematically uncovering the absorbed effective substances of Radix Scutellaria-licorice drug pair in rat plasma against COVID-19 using a combined UHPLC-Q-TOF-MS analysis and target network pharmacology

Therapeutic potential of flavonoids used in traditional Chinese medicine – a comparative study of galangin, kaempferol, chrysin and fisetin

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