Exploration of pharmacological interventions to prevent isoproterenol-induced myocardial infarction in experimental models

Garg, Monika; Kumari, Deepa

doi:10.1177/1753944714531638

Cited by 50 publications

(49 citation statements)

References 111 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Low Na + /K + -ATPase levels or activity promote Ca 2+ influx via NCX leading to Ca 2+ accumulation which can accelerate myocardial necrosis, contractile failure and also trigger arrhythmias. Calcium overload is one of the mechanisms of Iso-induced cardiac hypertrophy and heart failure ( Garg and Khanna, 2014 ) and also observed in our Iso treated H9C2 cells. Specifically, in cardiac hypertrophy, many studies have shown that Na + /K + pump function, and/or expression, is reduced.…”

Section: Discussionsupporting

confidence: 80%

Novel Sulfur Metabolites of Garlic Attenuate Cardiac Hypertrophy and Remodeling through Induction of Na+/K+-ATPase Expression

et al. 2017

View full text Add to dashboard Cite

Epidemiologic studies show an inverse correlation between garlic consumption and progression of cardiovascular disease. However, the molecular basis for the beneficial effect of garlic on the heart is not known. Therefore, the objective of the present study was to (1) investigate the effect of raw garlic on isoproterenol (Iso) induced cardiac hypertrophy (2) find the active metabolites of garlic responsible for the beneficial effect. Cardiac hypertrophy was induced in rats by subcutaneous single injection of Iso 5 mg kg-1 day-1 for 15 days and the effect of garlic (250 mg/kg/day orally) was evaluated. Garlic metabolites in in vivo were identified by LC/MS study. The effect of garlic and its metabolites were evaluated against hypertrophy in H9C2 cells. Garlic normalized cardiac oxidative stress after Iso administration. Cardiac pathology and mitochondrial enzyme activities were improved in hypertrophy heart after garlic administration. Decreased Na+/K+-ATPase protein level that observed in hypertrophy heart was increased after garlic administration. We identified three garlic metabolites in rat serum. To confirm the role of garlic metabolites on cardiac hypertrophy, Na+/K+-ATPase expression and intracellular calcium levels were measured after treating H9C2 cells with raw garlic and two of its active metabolites, allyl methyl sulfide and allyl methyl sulfoxide. Raw garlic and both metabolites increased Na+/K+-ATPase protein level and decreased intracellular calcium levels and cell size in Iso treated H9C2 cells. This antihypertrophic effect of garlic and its sulfur metabolites were lost in H9C2 cells in presence of Na+/K+-ATPase inhibitor. In conclusion, garlic and its active metabolites increased Na+/K+-ATPase in rat heart, and attenuated cardiac hypertrophy and associated remodeling. Our data suggest that identified new garlic metabolites may be useful for therapeutic intervention against cardiac hypertrophy.

show abstract

Section: Discussionsupporting

confidence: 80%

Novel Sulfur Metabolites of Garlic Attenuate Cardiac Hypertrophy and Remodeling through Induction of Na+/K+-ATPase Expression

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Interestingly, GE treatment in this study restored Na + /K + -ATPase activity and Ca 2+ levels towards normal values possibly by protecting the membrane structures via its antioxidant activity and/or control of its gene activity. Ca 2+ accumulation was reported as one of the mechanisms inducing cardiac hypertrophy in a myocardial infarction model (Garg and Khanna 2014). Therefore, the present ability of GE to prevent Ca 2+ overload could be one of the causes of the lower HW/BW ratio observed in GE and GNT co-treated animals than that of the GNT-treated animals in this study.…”

Section: Discussionmentioning

confidence: 52%

Garlic (Allium sativum) exhibits a cardioprotective effect in experimental chronic renal failure rat model by reducing oxidative stress and controlling cardiac Na+/K+-ATPase activity and Ca2+ levels

Gomaa

Abdelhafez

Aamer

2018

Cell Stress and Chaperones

View full text Add to dashboard Cite

Gentamicin (GNT)-induced nephrotoxicity culminates into renal failure with a possible cardiovascular impact. Garlic extract (GE) is a cardiovascular protectant with limited mechanistic data. Therefore, we assessed the disturbance in specific cardiac parameters and the potential protective effect of GE supplementation against them in a rat model of GNT-induced chronic renal failure (CRF). Adult male rats (n = 24) were randomly assigned into four groups (n = 6 each): normal controls (CON), garlic extract controls (GE; 250 mg kg, orally), GNT-induced CRF (GNT; 100 mg kg, i.p.), and GNT + GE (GNT and GE in the same previous doses) groups. GNT and GE were given daily for 3 weeks. Animals co-treated with GNT and GE exhibited improved renal functions, body weight (BW), and heart weight (HW)/BW ratio; declined blood pressure; lowered plasma levels of lactate dehydrogenase (LDH), creatine kinase-MB (CK-MB), and total peroxides (TP); and elevated total antioxidant capacity (TAC) levels. Moreover, the heart tissue contained raised levels of TAC and Na/K-ATPase activity and lowered levels of TP and Ca. Findings provide evidence that administration of GE in experimental CRF model helped protect the heart through reducing oxidative stress and controlling cardiac Na/K-ATPase activity and Ca levels.

show abstract

“…This might be due to the activation of NF-kB by ISO. Activation of NF-kB occurs via β-Adrenergic receptor dependent pathway by ISO 38 . In inflammation and apoptosis, NF-kB acts as a key regulator.…”

Section: Discussionmentioning

confidence: 99%

Combined cardio-protective ability of syringic acid and resveratrol against isoproterenol induced cardio-toxicity in rats via attenuating NF-kB and TNF-α pathways

Manjunatha

Shaik

Prasad

et al. 2020

Sci Rep

View full text Add to dashboard Cite

the study was conducted to evaluate the cardio-protective activity of combination (coMB) of syringic acid (SA) and resveratrol (RV) against isoproterenol (iSo) induced cardio-toxicity in rats. Rats were pretreated orally with SA (50 mg/kg), RV (50 mg/kg) and combination of SA (25 mg/kg) and RV (25 mg/kg) along with positive control gallic acid (50 mg/kg) for 30 days. The effects of ISO on cardiac markers, lipid profile and lipid peroxidation marker, anti-oxidant enzymes and m-RNA expression of nuclear factorkappa B (nf-kB) and tumor necrosis factor-α (tnf-α) were observed along with histopathological observations of simple and transmission electron microscopes (teM). Serum creatine kinase-MB (CK-MB), lactate dehydrogenase (LDH) and alkaline phosphatase were significantly increased while cardiac tissue CK-MB, LDH, superoxide dismutase and catalase were significantly decreased in ISO administered rats, which also exhibited a significant increase in total cholesterol, triglycerides, low density lipoprotein cholesterol, very low density lipoprotein cholesterol and thiobarbutyric acid reactive substances and significant decrease in high density lipoprotein cholesterol in serum and heart. The m-RNA levels of inflammatory markers NF-kB and TNF-α were significantly increased in ISO treated rats. COMB Pre-treatment significantly reversed the ISO actions. Histopathological studies of simple and teM were also co-related with the above biochemical parameters. Docking studies with nf-kB were also performed. Evidence has shown for the first time in this approach that COMB pre-treatment ameliorated iSo induced cardio-toxicity in rats and revealed cardio-protection. A worldwide health problem, coronary artery disease (CAD), very often leads to myocardial infarction (MI). Among the patients of coronary heart disease, the principal reason for mortality worldwide is MI. As expected by World Health Organization, in 2020, most of the deaths will occur due to MI 1. According to the Global Burden of Disease 2016 Study, cardiovascular diseases (CVD) alone accounted for 24% of total burden in men and 20% of total burden in women 2. Insufficient blood supply to the myocytes leads to MI that brings about changes in electrical, mechanical, basic and biochemical properties of heart 3. Isoproterenol (ISO) is a synthetic β-adrenergic agonist used to induce acute MI 4. The main mechanism of ISOinduced myocardial ischemia is free radicals production and reactive oxygen species (ROS), oxidative stress, lipid peroxidation (LPO) and overload of calcium (Ca 2+) etc 5 .

show abstract

Exploration of pharmacological interventions to prevent isoproterenol-induced myocardial infarction in experimental models

Cited by 50 publications

References 111 publications

Novel Sulfur Metabolites of Garlic Attenuate Cardiac Hypertrophy and Remodeling through Induction of Na+/K+-ATPase Expression

Novel Sulfur Metabolites of Garlic Attenuate Cardiac Hypertrophy and Remodeling through Induction of Na+/K+-ATPase Expression

Garlic (Allium sativum) exhibits a cardioprotective effect in experimental chronic renal failure rat model by reducing oxidative stress and controlling cardiac Na+/K+-ATPase activity and Ca2+ levels

Combined cardio-protective ability of syringic acid and resveratrol against isoproterenol induced cardio-toxicity in rats via attenuating NF-kB and TNF-α pathways

Contact Info

Product

Resources

About