Exploration of Predictive Biomarkers for Postoperative Recurrence in Chronic Rhinosinusitis with Nasal Polyps Based on Serum Multiple-Cytokine Profiling

Abstract

Background. Functional nasal endoscopic surgery (FESS) is an effective treatment approach for chronic rhinosinusitis with nasal polyps (CRSwNP) patients, but some patients still suffer from postoperative recurrence. This study is aimed at investigating the expression of multiple cytokines in CRSwNP and revealing their relationships with postoperative recurrence. Methods. A total of 72 patients with CRSwNP, including 36 primary and 36 recurrent patients, were enrolled. Serum samples were obtained, 30 cytokine levels were measured by multiplex analysis, and the association between cytokine levels and recurrence was assessed. The most potential cytokines were further validated in another independent cohort with 60 primary and 60 recurrent CRSwNP patients. Results. The results of multiple cytokine profiling exhibited that the levels of eotaxin, G-CSF, IFN-α, IL-13, IL-17A, IL-5, MCP-1, and RANTES were vastly changed in the recurrent group in comparison with the primary group. Receiver-operating characteristic (ROC) curves highlighted that serum levels of eotaxin, IL-17A, and RANTES were strongly predictive of postoperative recurrence ( area under the curve AUC > 0.7 , P < 0.05 ). Further validation results showed that elevated serum eotaxin, IL-17A, and RANTES levels were enhanced in the recurrent group. The ROC curve showed that serum eotaxin ( AUC = 0.729 , P < 0.001 ) and RANTES ( AUC = 0.776 , P < 0.001 ) exhibited stronger ability than serum IL-17A ( AUC = 0.617 , P = 0.027 ) in predicting CRSwNP recurrence. Conclusion. Our data suggested that serum multiple cytokine profiling was associated with postoperative recurrence of CRSwNP, and eotaxin and RANTES might serve as potential biomarkers for predicting postoperative recurrence. These results might contribute to the understanding of the underlying mechanisms of recurrence and provide novel clues for precision therapy in CRSwNP.

Serum proteomics identify CSF1R as a novel biomarker for postoperative recurrence in chronic rhinosinusitis with nasal polyps

“…Although recent studies have advanced our understanding of the mechanisms involved in recurrent CRSwNP and shed light on postoperative recurrence mechanisms. These studies have delved into various aspects, including circulating and tissue cytokines, 28 metabolites, 9 , 29 and nasal microbiome. 30 However, despite these advancements, the complexities underlying postoperative recurrence mechanisms remain not completely elucidated.…”

Outcome measures for chronic rhinosinusitis with nasal polyps

“…• May be used as a biomarker for predicting post-operative recurrence (449,450) Glucocorticoid Receptor β (GRβ)…”

Cytokine profile of nasal polyps and clinical characteristics of patients with polypous rhinosinisitis at different degree of therapeutic control and disease severity

“…Другие коллеги изучали мультиплексным анализом содержание 30 цитокинов в сыворотке крови. В группе с рецидивирующим ПРС определялся повышенный уровень эотоксинов 1-3, IL-17A, играющих критическую роль в агрегации эозинофилов, и хемокина RANTES (regulated upon activation normal T-cell expressed and secreted) [22]. Повышенный в 3 раза уровень эотаксина-3 крови при частых рецидивах ПРС был отмечен и при другом исследовании [23].…”