Exploration of serum biomarkers for predicting the response to Inchinkoto (ICKT), a Japanese traditional herbal medicine

Uji, Masahito; Yokoyama, Yukihiro; Ohbuchi, Katsuya; Tsuchiya, Kiichiro; Sadakane, Chiharu; Shimobori, Chika; Yamamoto, Masahiro; Nagino, Masato

doi:10.1007/s11306-017-1292-x

Cited by 10 publications

(14 citation statements)

References 42 publications

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“…Significant correlations between the levels of ICKT components (e.g., geniposide, dimethylesculetin, and conjugated genipin) in the serum on days 1 and 4 and changes in hepatobiliary markers were not observed. This finding was consistent with those of a previous study (Uji et al, 2017).…”

Section: Correlation Analysissupporting

confidence: 94%

“…Pearson correlation analysis was performed to explore relationships among the serum endogenous metabolites, ICKT components, and hepatobiliary markers. A previous study reported that the serum total bilirubin was closely correlated with several serum metabolites (Uji et al, 2017). The present study also demonstrated that the levels of 20-carboxyarachidonic acid and cystathionine were closely correlated with those of serum total bilirubin on days 1 and 4 (Fig.…”

Section: Correlation Analysissupporting

confidence: 84%

“…The concentrations of the main components of ICKT in the serum were determined using liquid chromatographytandem mass spectrometry (LC-MS/MS) according to the method described by Uji et al (Uji et al, 2017). The instruments consisted of an Agilent 1290 UPLC system (Agilent Technologies, Santa Clara, CA, USA) with a 6500 QTRAP® triple quadrupole mass spectrometer (AB Sciex, Framingham, MA, USA).…”

Section: Quantitative Analysis Of the Concentrations Of Ickt Components In The Serummentioning

confidence: 99%

“…In a previous study, we performed serum metabolome analysis to elucidate the mechanism involved in the pharmaceutical action of ICKT (Uji et al, 2017). Although the levels of lipid mediators and hydrophilic metabolites were significantly changed after the administration of ICKT, the analysis was unable to exclude the effects of diet.…”

Section: Introductionmentioning

confidence: 99%

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Comprehensive metabolome analysis for the pharmacological action of inchinkoto, a hepatoprotective herbal medicine

et al. 2021

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IntroductionThe precise pharmacological action of inchinkoto (ICKT, Yin-Chen-Hao-Tang in Chinese), a hepatoprotective herbal medicine, on total metabolic pathways has not been well investigated.Objectives The aim of this study was to explore the serum metabolites reflecting the pharmacological activity of ICKT, and mechanism of action of ICKT using serum metabolome analysis. Methods 54 patients with obstructive jaundice due to malignancies were included in this study. ICKT was administered for 3 days. Serum and bile samples were collected before and 1 h after ICKT administration on days 1 and 4. Serum metabolome analysis including ICKT components were performed. ResultsThe levels of total/direct bilirubin, C-reactive protein, γ-glutamyl transpeptidase, and albumin in the serum were significantly improved after ICKT administration. In the serum metabolome analysis, inosine was the only elevated metabolite on days 1 and 4. Most of the metabolites which were significantly changed after ICKT administration were lipid mediators, and all decreased on day 1. Notably, the levels of many lipid mediators were increased on day 4. The difference in serum aspartic acid 1 h after ICKT administration was significantly correlated with a decrease in the levels of total bilirubin in the serum on day 4. Conclusions Using metabolome analysis, we demonstrated several metabolic changes that may be associated with the pharmacological mechanisms of ICKT. The biological implications of these metabolites should be further investigated in basic research studies.

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Section: Correlation Analysissupporting

confidence: 94%

Section: Correlation Analysissupporting

confidence: 84%

Section: Quantitative Analysis Of the Concentrations Of Ickt Components In The Serummentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comprehensive metabolome analysis for the pharmacological action of inchinkoto, a hepatoprotective herbal medicine

et al. 2021

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“…GC/MS was carried out using GCMS-TQ8040 (Shimadzu Co., Kyoto, Japan) with a DB-5 capillary column (30 m × 0.25 mm i.d. ; 1 μm film thickness; Agilent J & W Scientific, Folsom, CA, USA) as described previously [ 26 ]. The inlet temperature was set at 250 °C, and the injection volume was 1 μL (splitless mode).…”

Section: Methodsmentioning

confidence: 99%

BDK Deficiency in Cerebral Cortex Neurons Causes Neurological Abnormalities and Affects Endurance Capacity

et al. 2020

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Branched-chain amino acid (BCAA) catabolism is regulated by its rate-limiting enzyme, branched-chain α-keto acid dehydrogenase (BCKDH), which is negatively regulated by BCKDH kinase (BDK). Loss of BDK function in mice and humans leads to dysregulated BCAA catabolism accompanied by neurological symptoms such as autism; however, which tissues or cell types are responsible for the phenotype has not been determined. Since BDK is highly expressed in neurons compared to astrocytes, we hypothesized that neurons are the cell type responsible for determining the neurological features of BDK deficiency. To test this hypothesis, we generated mice in which BDK deletion is restricted to neurons of the cerebral cortex (BDKEmx1-KO mice). Although BDKEmx1-KO mice were born and grew up normally, they showed clasped hind limbs when held by the tail and lower brain BCAA concentrations compared to control mice. Furthermore, these mice showed a marked increase in endurance capacity after training compared to control mice. We conclude that BDK in neurons of the cerebral cortex is essential for maintaining normal neurological functions in mice, and that accelerated BCAA catabolism in that region may enhance performance in running endurance following training.

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Data on metabolic profiling of healthy human subjects’ plasma before and after administration of the Japanese Kampo medicine maoto

et al. 2019

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This data article contains the data on metabolic profiling of healthy human subjects’ plasma before and after administration of the Japanese Kampo medicine maoto. Four healthy human subjects were recruited. Plasma samples were collected before and 0.25, 0.5, 1, 2, 4 and 8 h after maoto treatment. Endogenous and exogenous compounds in plasma were analyzed using MS. Endogenous compounds including saccharides, amino acids, organic acids and other hydrophilic metabolites were semi-quantitatively measured using GC-MS/MS. Lipid mediators such as arachidonic acid, docosahexaenoic acid and eicosapentaenoic acid were semi-quantitatively measured using LC-MS/MS. Maoto constituents in plasma were quantitatively measured using LC-MS/MS. The data files contain the area ratio values, which were normalized to the intensity of the internal standard, and plasma concentration of maoto compounds. The data article is related to the research article titled “Phenotyping analysis of the Japanese Kampo medicine maoto in healthy human subjects using wide-targeted plasma metabolomics” (Kitagawa et al., 2018).

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Exploration of serum biomarkers for predicting the response to Inchinkoto (ICKT), a Japanese traditional herbal medicine

Cited by 10 publications

References 42 publications

Comprehensive metabolome analysis for the pharmacological action of inchinkoto, a hepatoprotective herbal medicine

Comprehensive metabolome analysis for the pharmacological action of inchinkoto, a hepatoprotective herbal medicine

BDK Deficiency in Cerebral Cortex Neurons Causes Neurological Abnormalities and Affects Endurance Capacity

Data on metabolic profiling of healthy human subjects’ plasma before and after administration of the Japanese Kampo medicine maoto

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