Exploration of Sugar and Starch Metabolic Pathway Crucial for Pollen Fertility in Rice

Lee, Sang‐Kyu; Lee, Juho; Jo, Mingyu; Jeon, Jong‐Seong

doi:10.3390/ijms232214091

Cited by 17 publications

(6 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In plant tubers, the main sites for starch synthesis are chloroplasts and amyloplasts. Within the chloroplast stroma, the Calvin cycle utilizes CO 2 , which enters the chloroplast matrix through the stomata ( Lee et al, 2022 ). Initially, it forms 3-phosphoglycerate (3PG) by reacting with its receptor RuBP under the influence of Rubisco carboxylase.…”

Section: Introductionmentioning

confidence: 99%

“…2 ) ( Zhu et al, 2022 ). Finally, ADP-glucose produces amylopectin through the activities of starch isomerase, starch branching enzyme, and soluble starch synthase, while another portion is transformed into amylose by granule-bound starch synthase and stored in endosperm cells ( Lee et al, 2022 ). In sugar metabolism, sucrose phosphate synthase (SPS) and sucrose phosphatase (SPP) are present as compounds in plants, and the process of sucrose production catalyzed by SPS is irreversible ( Worden et al, 2015 ; Mason et al, 2023 ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Research overview on the genetic mechanism underlying the biosynthesis of polysaccharide in tuber plants

Xu,

Hu,

et al. 2024

PeerJ

View full text Add to dashboard Cite

Tuber plants are of great significance in the world as human food crops. Polysaccharides, important metabolites in tuber plants, also serve as a source of innovative drugs with significant pharmacological effects. These drugs are particularly known for their immunomodulation and antitumor properties. To fully exploit the potential value of tuber plant polysaccharides and establish a synthetic system for their targeted synthesis, it is crucial to dissect their metabolic processes and genetic regulatory mechanisms. In this article, we provide a comprehensive summary of the basic pathways involved in the synthesis of various types of tuber plant polysaccharides. We also outline the key research progress that has been made in this area in recent years. We classify the main types and functions of tuber plant polysaccharides and analyze the biosynthetic processes and genetic regulation mechanisms of key enzymes involved in the metabolic pathways of starch, cellulose, pectin, and fructan in tuber plants. We have identified hexokinase and glycosyltransferase as the key enzymes involved in the polysaccharide synthesis process. By elucidating the synthesis pathway of polysaccharides in tuber plants and understanding the underlying mechanism of action of key enzymes in the metabolic pathway, we can provide a theoretical framework for enhancing the yield of polysaccharides and other metabolites in plant culture cells. This will ultimately lead to increased production efficiency.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Research overview on the genetic mechanism underlying the biosynthesis of polysaccharide in tuber plants

Xu,

Hu,

et al. 2024

PeerJ

View full text Add to dashboard Cite

show abstract

“…Starch, a prominent polysaccharide stored in mature pollen grains of many crop plants, is essential for pollen germination and tube growth. Deficiency in starch accumulation leads to the blockage of pollen germination and pollen tube growth due to the shortage of energy and membrane material provision (Lee et al 2022). Thus, the dynamic interplay between starch content and viability becomes evident through the profound influence of starch hydrolysis on pollen lifespan and emission timing (Pacini and Hesse 2002).…”

Section: Introductionmentioning

confidence: 99%

Dynamic organelle changes and autophagic processes in lily pollen germination

Yen,

Hsu,

Jiang

et al. 2024

Bot Stud

View full text Add to dashboard Cite

Pollen germination is a crucial process in the life cycle of flowering plants, signifying the transition of quiescent pollen grains into active growth. This study delves into the dynamic changes within organelles and the pivotal role of autophagy during lily pollen germination. Initially, mature pollen grains harbor undifferentiated organelles, including amyloplasts, mitochondria, and the Golgi apparatus. However, germination unveils remarkable transformations, such as the redifferentiation of amyloplasts accompanied by starch granule accumulation. We investigate the self-sustained nature of amylogenesis during germination, shedding light on its association with osmotic pressure. Employing BODIPY 493/503 staining, we tracked lipid body distribution throughout pollen germination, both with or without autophagy inhibitors (3-MA, NEM). Typically, lipid bodies undergo polarized movement from pollen grains into elongating pollen tubes, a process crucial for directional growth. Inhibiting autophagy disrupted this essential lipid body redistribution, underscoring the interaction between autophagy and lipid body dynamics. Notably, the presence of tubular endoplasmic reticulum (ER)-like structures associated with developing amyloplasts and lipid bodies implies their participation in autophagy. Starch granules, lipid bodies, and membrane remnants observed within vacuoles further reinforce the involvement of autophagic processes. Among the autophagy inhibitors, particularly BFA, significantly impede germination and growth, thereby affecting Golgi morphology. Immunogold labeling substantiates the pivotal role of the ER in forming autophagosome-like compartments and protein localization. Our proposed speculative model of pollen germination encompasses proplastid differentiation and autophagosome formation. This study advances our understanding of organelle dynamics and autophagy during pollen germination, providing valuable insights into the realm of plant reproductive physiology.

show abstract

“…Similarly, a 598-bp InDel variation in the promoter region of Bna.SOC1.A05 has a huge impact on mRNA expression and is associated with winter types in rapeseeds [ 17 ]. Both in plants and animals, InDel mutations were highly enriched in the driving region of gene and associated with differential gene expression and changes [ 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analyses Show Changes in Gene Expression Triggered by a 31-bp InDel within OsSUT3 5′UTR in Rice Panicle

Zhang

Wen

et al. 2023

IJMS

View full text Add to dashboard Cite

Pollen development and its fertility are obligatory conditions for the reproductive success of flowing plants. Sucrose transporter 3 (OsSUT3) is known to be preferentially expressed and may play critical role in developing pollen. A 31-bp InDel was identified as a unique variation and was shown to be responsible for the expression of downstream gene in our previous study. In this study, to analyze the changes of gene expression triggered by 31-bp InDel during pollen development, two vectors (p385-In/Del::OsSUT3-GUS) were constructed and then stably introduced into rice. Histochemical and quantitative real-time PCR (qRT-PCR) analysis of transgenic plants showed that 31-bp deletion drastically reduced the expressions of downstream genes, including both OsSUT3 and GUS in rice panicle at booting stage, especially that of OsSUT3. The transcriptome profile of two types of panicles at booting stage revealed a total of 1028 differentially expressed genes (DEGs) between 31-bp In and 31-bp Del transgenic plants. Further analyses showed that 397 of these genes were significantly enriched for the ‘metabolic process’ and ‘binding’. Among them, nineteen genes had a strong relationship with starch and sucrose metabolism and were identified as candidate genes potentially associated with the starch accumulation in rice pollen, which that was also verified via qRT-PCR. In summary, 31-bp InDel plays a crucial role not only in the regulation of downstream genes but in the expression of sucrose-starch metabolizing genes in multiple biological pathways, and provides a different regulation mechanism for sucrose metabolism in pollen.

show abstract

Exploration of Sugar and Starch Metabolic Pathway Crucial for Pollen Fertility in Rice

Cited by 17 publications

References 52 publications

Research overview on the genetic mechanism underlying the biosynthesis of polysaccharide in tuber plants

Research overview on the genetic mechanism underlying the biosynthesis of polysaccharide in tuber plants

Dynamic organelle changes and autophagic processes in lily pollen germination

Transcriptome Analyses Show Changes in Gene Expression Triggered by a 31-bp InDel within OsSUT3 5′UTR in Rice Panicle

Contact Info

Product

Resources

About