Exploration of the effect of blue light on microRNAs involved in the accumulation of functional metabolites of longan embryonic calli through RNA‐sequencing

Abstract

The results of the study revealed that the expressions of phase-specific miRNAs vary with the change of functional metabolites in longan ECs. This study provides new insights into the molecular mechanisms that allow light to influence plant metabolism. © 2018 Society of Chemical Industry.

Exploration of the Effect of Blue Light on Functional Metabolite Accumulation in Longan Embryonic Calli via RNA Sequencing

“…DEGs that regulate starch and sucrose were found in the DB and DW comparisons (Figure 5A,B) and the expression levels of most genes in the blue light treatment group were higher than those in the other groups (Figure 5C). These results further confirm the contribution of blue light to biotin and polysaccharide synthesis in longan ECs, which is consistent with the results of Li et al [23].…”

“…In addition, heat map analysis of DEGs demonstrated that the expression levels of most genes in secondary metabolic pathways were significantly higher under blue light treatment than under the other treatments (Figure 6C). The above results indicate that blue light is most beneficial for promoting the accumulation of carotenoids, alkaloids and total flavonoids in longan ECs, which is consistent with the results of Li et al [23].…”

“…Light can promote the generation of polysaccharides, biotin, alkaloids and flavonoids in longan ECs and blue light promotes these four functional metabolites better than other light treatments [23]. Herein, we showed that the carotenoid content was highest (17.82 μg·g −1 ) in the blue light treatment group, followed by white light and lowest in the darkness group (11.32 μg·g −1 ) (Figure 1A and Table S1).…”

“…Previous studies published by our laboratory showed that different light qualities affect the growth state and functional metabolites of longan ECs and revealed that blue light is optimal for the synthesis of longan EC functional metabolites [22,23]; darkness was used as a control. However, comparing blue and white light treatments is useful for understanding the difference between monochromatic and composite light and the difference in light quality.…”

RNA sequencing-based exploration of the effects of far-red light on lncRNAs involved in the shade-avoidance response ofD. officinale

“…Freeze-dried grains of D. officinale stems and leaves were extracted as previously described (Li et al, 2018). The absorbance was measured with a UV-visible spectrophotometer (Evolution 350, Thermo Fisher, MA, USA), and the wavelengths of polysaccharides, flavonoids and alkaloids were measured at 485 nm, 510 nm and 416 nm, respectively (Li et al, 2018). The polysaccharide, flavonoid and alkaloid contents in the D. officinale stems and leaves were calculated according to established standard curves.…”