Exploration of the influence of surface proteins on the probiotic activity of Lactobacillus pentosus HC-2 in the Litopenaeus vannamei midgut via label-free quantitative proteomic analysis

Abstract

Our previously work showed that Lactobacillus pentosus HC-2 as probiotic could improve the growth performance, immune response, gut bacteria diversity and disease resistance of Litopenaeus vannamei. However, the probiotic mechanism of was not fully characterized. In the present study, histology and proteomic analysis was performed to explore the influence of HC-2 surface protein on its probiotic effects to L. vannamei after feeding the intact surface proteins or the probiotic treated with lithium chloride (LiCl) to remove non-covalently bound surface proteins or no probiotic for four weeks.Histological observation found that feeding with normal HC-2 obviously improved the intestinal histology and enhanced the protective effect against pathogens damages, but fed with LiCl-treated HC-2 didn't improve the intestinal environment. A total over 2,764 Peptides and 1,118 uniproteins were identified from L. vannamei midgut, 211 proteins were significant differentially expressed normal HC-2 group compared with control, 510 proteins were significant differentially expressed in LiCl-tread HC-2 group compared with control, and 458 proteins were significant differentially expressed in LiCltread HC-2 group compared with normal HC-2 group. GO/KEGG enrichment analysis of the significantly different proteins demonstrated that fed with normal HC-2 mainly induced immune response, metabolic, cell adhesion and cell-cell signaling related proteins up-regulation, which were contributed to bacteria adhesion and colonization in midgut to improve shrimp immune system and growth, but these proteins were suppressed after feeding with deprived surface proteins bacteria. Taken together, these results indicating that the surface proteins were indispensable for HC-2 to execute probiotic effects in midgut of shrimp.

Alginate Co-probiotics Bioencapsulation Facilitates Artemia to Improve Immune Parameters, Gene Expression, Feed Digestibility and Resistance Against Vibrio spp

“…Our previous results showed similar facts (Yudiati et al 2016(Yudiati et al , 2019. The quantitative proteomic analysis and intestinal histology of L. pentosus probiotic activity were noted (Du et al 2019a). The surface proteins of L. pentosus signi cantly in uenced the protein transcription in the shrimp gut.…”

Alginate Co-probiotics Bioencapsulation Facilitates Artemia to Improve Immune Parameters, Gene Expression, Feed Digestibility and Resistance Against Vibrio spp

“…5.6 mm size in 1 h/individu. The most e cient digestive tract are individuals larger than 5.6 mmDu et al (2019a) was strengthened our results. LAB predominantly convinced metabolic and cell to cell signaling-related protein upregulation.…”

Effects of different dietary preparations of Enterococcus faecalis F7 on the growth and intestinal microbiota of Pacific white shrimp ( Litopenaeus vannamei )

“…In some studies, cell‐free extract or fermentation supernatant of Lactobacillus plantarum was also found to be beneficial to the host (Zheng et al, 2017, 2020). Additionally, Lactobacillus pentosus HC‐2 treated with lithium chloride (LiCl) to eliminate non‐covalently bound surface proteins has an effect on probiotic actions in the shrimp midgut (Du et al, 2019). However, our study found that L .…”

“…In some studies, cell-free extract or fermentation supernatant of Lactobacillus plantarum was also found to be beneficial to the host (Zheng et al, , 2020. Additionally, Lactobacillus pentosus HC-2 treated with lithium chloride (LiCl) to eliminate non-covalently bound surface proteins has an effect on probiotic actions in the shrimp midgut (Du et al, 2019). However, our study found that L. vannamei fed with live E. faecalis F7 preparation diets performed better in terms of weight gain rate (WGR), specific growth rate (SGR) and feed conversion ratio (FCR) when compared to that fed with a regular diet, dead bacteria, cell-free extract of E. faecalis F7 and E. faecalis F7 treated with LiCl (Figure 1).…”