Exploration of The Mechanism of Qianliexin Capsule Against Chronic Nonbacterial Prostatitis Using Metabolomicsv

Zang, Lihua; Y, Zhang; Zhang, M; Yang, Xuesong; Y, Li; J, Zhang; Chen, Yi; Shang, Yangyang; Wang, Pei‐Kang; Yang, Zongbao; Zhou, Lai; Chi, Yonggang; Meng, Zhaoqing; J, Lu

doi:10.47829/cos.2021.6501

Cited by 124 publications

(169 citation statements)

References 29 publications

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“…Due to the low sample size, correlation analysis of age and CRAE and CRVE was not conducted. Normoxic retinal vessel diameters measured in children and adults participating in the present study are smaller than the values reported in the literature (Mitchell et al, 2007;Li et al, 2011;Neumann et al, 2016). A similar phenomenon was observed in adults' CRAE and CRVE measurements during exposure to hypoxia (Neumann et al, 2016).…”

Section: Adults Vs Childrensupporting

confidence: 63%

Retinal blood vessel diameters in children and adults exposed to a simulated altitude of 3,000 m

Mlinar

Debevec²,

Kapus³

et al. 2023

Front. Physiol.

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Introduction: Technological advances have made high-altitude ski slopes easily accessible to skiers of all ages. However, research on the effects of hypoxia experienced during excursions to such altitudes on physiological systems, including the ocular system, in children is scarce. Retinal vessels are embryologically of the same origin as vessels in the brain, and have similar anatomical and physiological characteristics. Thus, any hypoxia-related changes in the morphology of the former may reflect the status of the latter.Objective: To compare the effect of one-day hypoxic exposure, equivalent to the elevation of high-altitude ski resorts in North America and Europe (∼3,000 m), on retinal vessel diameter between adults and children.Methods: 11 adults (age: 40.1 ± 4.1 years) and 8 children (age: 9.3 ± 1.3 years) took part in the study. They spent 3 days at the Olympic Sports Centre Planica (Slovenia; altitude: 940 m). During days 1 and 2 they were exposed to normoxia (FiO2 = 0.209), and day 3 to normobaric hypoxia (FiO2 = 0.162 ± 0.03). Digital high-resolution retinal fundus photographs were obtained in normoxia (Day 2) and hypoxia (Day 3). Central retinal arteriolar equivalent (CRAE) and venular equivalents (CRVE) were determined using an Automated Retinal Image Analyser.Results: Central retinal arteriolar and venular equivalents increased with hypoxia in children (central retinal arteriolar equivalent: 105.32 ± 7.72 µm, hypoxia: 110.13 ± 7.16 µm, central retinal venular equivalent: normoxia: 123.39 ± 8.34 µm, hypoxia: 130.11 ± 8.54 µm) and adults (central retinal arteriolar equivalent: normoxia: 105.35 ± 10.67 µm, hypoxia: 110.77 ± 8.36 µm; central retinal venular equivalent: normoxia: 126.89 ± 7.24 µm, hypoxia: 132.03 ± 9.72 µm), with no main effect of group or group*condition interaction. A main effect of condition on central retinal arteriolar and venular equivalents was observed (central retinal arteriolar equivalent:normoxia: 105.34 ± 9.30 µm, hypoxia: 110.50 ± 7.67 µm, p < 0.001; central retinal venular equivalent: normoxia: 125.41 ± 7.70 µm, hypoxia: 131.22 ± 9.05 µm, p < 0.001).Conclusion: A 20-hour hypoxic exposure significantly increased central retinal arteriolar and venular equivalents in adults and children. These hypoxia-induced increases were not significantly different between the age groups, confirming that vasomotor sensitivity of the retinal vessels to acute hypoxia is comparable between adults and prepubertal children.

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Section: Adults Vs Childrensupporting

confidence: 63%

Retinal blood vessel diameters in children and adults exposed to a simulated altitude of 3,000 m

Mlinar

Debevec²,

Kapus³

et al. 2023

Front. Physiol.

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“…60 A murine study found over-expression of PRX-2 attenuated oxidative stress and neuronal apoptosis following subarachnoid haemorrhage. 61 Over-expression of PRX-2 is speculated to protect again ischaemic neuronal injury by modulating the redox-sensitive thioredoxin-apoptosis signal-regulating kinase (ASK) 1 signalling complex. 62 Several molecular chaperones can interact with ASK1, including thioredoxin and TNF receptor-associated factor 6.…”

Section: Discussionmentioning

confidence: 99%

A comprehensive proteomic and bioinformatics analysis of human spinal cord injury plasma identifies proteins associated with the complement cascade and liver function as potential prognostic indicators of neurological outcome

Harrington

Cool

Hulme

et al. 2022

Preprint

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Introduction: Spinal Cord Injury (SCI) is a major cause of disability, with complications post-injury often leading to life-long health issues with need of extensive treatment. Neurological outcome post-SCI can be variable and difficult to predict, particularly in incomplete injured patients. The identification of specific SCI biomarkers in blood, may be able to improve prognostics in the field. This study has utilised proteomic and bioinformatics methodologies to investigate differentially expressed proteins in plasma samples across human SCI cohorts with the aim of identifying prognostic biomarkers and biological pathway alterations that relate to neurological outcome. Methods and Materials: Blood samples were taken, following informed consent, from ASIA impairment scale (AIS) grade C "Improvers" (those who experienced an AIS grade improvement) and "Non-Improvers" (No AIS change), and AIS grade A and D at <2 weeks ("Acute") and approx. 3 months ("Sub-acute") post-injury. The total protein concentration from each sample was extracted, with pooled samples being labelled and non-pooled samples treated with ProteoMiner™ beads. Samples were then analysed using two 4-plex isobaric tag for relative and absolute quantification (iTRAQ) analyses and a label-free experiment for comparison, before quantifying with mass spectrometry. Data are available via ProteomeXchange with identifiers PXD035025 and PXD035072 for the iTRAQ and label-free experiments respectively. Proteomic datasets were analysed using OpenMS (version 2.6.0). R (version 4.1.4) and in particular, the R packages MSstats (version 4.0.1) and pathview (version 1.32.0) were used for downstream analysis. Proteins of interest identified from this analysis were further validated by enzyme-linked immunosorbent assay (ELISA). Results: The data demonstrated proteomic differences between the cohorts, with the results from the iTRAQ approach supporting those of the label-free analysis. A total of 79 and 87 differentially abundant proteins across AIS and longitudinal groups were identified from the iTRAQ and label-free analyses, respectively. Alpha-2-macroglobulin (A2M), retinol binding protein 4 (RBP4), serum amyloid A1 (SAA1), Peroxiredoxin 2, alipoprotein A1 (ApoA1) and several immunoglobulins were identified as biologically relevant and differentially abundant, with potential as individual prognostic biomarkers of neurological outcome. Bioinformatics analyses revealed that the majority of differentially abundant proteins were components of the complement cascade and most interacted directly with the liver. Conclusions: Many of the proteins of interest identified using proteomics were detected only in a single group and therefore have potential as a binary (present or absent) biomarkers, RBP4 and PRX-2 in particular. Additional investigations into the chronology of these proteins, and their levels in other tissues (cerebrospinal fluid in particular) are needed to better understand the underlying pathophysiology, including any potentially modifiable targets. Pathway analysis highlighted the complement cascasde as being significant across groups of differential functional recovery.

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“…The mixture is heated in a boiling water bath for 30 min and then centrifuged for 10 min. The absorbance of the supernatant was measured at 450 nm, 532 nm and 600 nm (Liu et al, 2022b). For estimating O •− 2 generation, the samples were homogenized with potassium phosphate buffer (pH 7.8) and centrifuged for 10 min.…”

Section: Endogenous No Contentmentioning

confidence: 99%

Nitric oxide promotes adventitious root formation in cucumber under cadmium stress through improving antioxidant system, regulating glycolysis pathway and polyamine homeostasis

Niu

Tang²,

Zhu³

et al. 2023

Front. Plant Sci.

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Cadmium (Cd) as a potentially toxic heavy metal that not only pollutes the environment but also interferes with plant growth. Nitric oxide (NO) regulates plant growth and development as well as abiotic stress response. However, the mechanism underpinning NO-induced adventitious root development under Cd stress remains unclear. In this study, cucumber (Cucumis sativus ‘Xinchun No. 4’) was used as the experimental material to investigate the effect of NO on the development of adventitious roots in cucumber under Cd stress. Our results revealed that, as compared to Cd stress, 10 μM SNP (a NO donor) could considerably increase the number and length of adventitious roots by 127.9% and 289.3%, respectively. Simultaneously, exogenous SNP significantly increased the level of endogenous NO in cucumber explants under Cd stress. Our results revealed that supplementation of Cd with SNP significantly increased endogenous NO content by 65.6% compared with Cd treatment at 48 h. Furthermore, our study indicated that SNP treatment could improve the antioxidant capacity of cucumber explants under Cd stress by up-regulating the gene expression level of antioxidant enzymes, as well as reducing the levels of malondialdehyde (MDA), hydrogen peroxide (H2O2) and superoxide anion (O2.-) to alleviate oxidative damage and membrane lipid peroxidation. Application of NO resulted in a decrease of the O2.-, MDA, and H2O2 level by 39.6%, 31.4% and 60.8% as compared to Cd-alone treatment, respectively. Besides that, SNP treatment significantly increased the expression level of related genes involved in glycolysis processes and polyamine homeostasis. However, application of NO scavenger 2-(4-carboxy -2-phenyl)-4, 4, 5, 5-tetramethy limidazoline -1-oxyl -3-oxide (cPTIO) and the inhibitor tungstate significantly reversed the positive role of NO in promoting the adventitious root formation under Cd stress. These results suggest that exogenous NO can increase the level of endogenous NO, improve antioxidation ability, promote glycolysis pathway and polyamine homeostasis to enhance the occurrence of adventitious roots in cucumber under Cd stress. In summary, NO can effectively alleviate the damage of Cd stress and significantly promote the development of adventitious root of cucumber under Cd stress.

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Exploration of The Mechanism of Qianliexin Capsule Against Chronic Nonbacterial Prostatitis Using Metabolomicsv

Cited by 124 publications

References 29 publications

Retinal blood vessel diameters in children and adults exposed to a simulated altitude of 3,000 m

Retinal blood vessel diameters in children and adults exposed to a simulated altitude of 3,000 m

A comprehensive proteomic and bioinformatics analysis of human spinal cord injury plasma identifies proteins associated with the complement cascade and liver function as potential prognostic indicators of neurological outcome

Nitric oxide promotes adventitious root formation in cucumber under cadmium stress through improving antioxidant system, regulating glycolysis pathway and polyamine homeostasis

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