Exploration of three heterotrophic nitrifying strains from a tilapia pond for their characteristics of inorganic nitrogen use and application in aquaculture water

Fan, Limin; Chen, Jiazhang; Liu, Qi; Wu, Wei; Meng, Shunlong; Song, Chao; Jianhong, Qu; Xu, Pao

doi:10.1016/j.jbiosc.2014.09.006

Cited by 11 publications

(11 citation statements)

References 20 publications

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“…The newly discovered functionality of the strain C. amphilecti reported in this article has thus usefully broadened the range of autochthonous strains known to remove ammonia-N. and A. baumannii isolated from tilapia ponds removed 67.9 and 76.7% of the total ammonia-N after 24 hr (Fan et al, 2015), respectively; and Marinobacter sp. and A. baumannii isolated from tilapia ponds removed 67.9 and 76.7% of the total ammonia-N after 24 hr (Fan et al, 2015), respectively; and Marinobacter sp.…”

Section: Discussionmentioning

confidence: 93%

“…Although a number of potential strains that have been isolated from aquaculture systems have been shown to remove ammonia-N, including Pseudomonas sp., Acinetobacter baumannii, and Rhodopseudamonas palustris (Fan et al, 2015;Zhang, Gao et al, 2014;Zhang, Shu et al, 2014), these species are quite rare. The newly discovered functionality of the strain C. amphilecti reported in this article has thus usefully broadened the range of autochthonous strains known to remove ammonia-N.…”

Section: Discussionmentioning

confidence: 99%

“…There have been many reports of the efficiency of various strains in removing ammonia-N. For example, Bacillus strains have been shown to remove 22.5-28.9% of ammonia-N in L. vannamei ponds (Subuntith, Sunisa, Traimat, & Verapong, 2012); Pseudomonas sp. and A. baumannii isolated from tilapia ponds removed 67.9 and 76.7% of the total ammonia-N after 24 hr (Fan et al, 2015), respectively; and Marinobacter sp. removed 48.6% ammonia-N in growth medium after 5 days (Zheng et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

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A novel micro‐organism for removing excess ammonia‐N in seawater ponds and the effect of Cobetia amphilecti on the growth and immune parameters of Litopenaeus vannamei

Pan

Zhang

et al. 2018

J World Aquaculture Soc

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To investigate the feasibility of using micro‐organisms for ammonia‐N removal, six strains were isolated from Chinese white shrimp, Fenneropenaeus chinensis, seawater culture ponds in Dongying (Shandong, China). Of these, strain DY‐01, which exhibited the highest capacity to degrade ammonia‐N, removed 61.7% of the total ammonia‐N (50 mg/L) in 8 hr. An investigation of the factors affecting the removal efficiency indicated the optimum conditions to be 30°C, pH 8.0, and a salinity level of 30 g/L; 16S rDNA gene sequencing and biochemical analysis identified strain DY‐01 as Cobetia amphilecti, which has not previously been reported to degrade ammonia‐N. This strain also boosted the growth of Pacific white shrimp, Litopenaeus vannamei (p < .05), at a concentration of 107 CFU/mL, with no harmful effects on the shrimp's immune system. This study has thus identified a novel aerobic nitrifying bacterium that is potentially an excellent candidate for improving the water quality in mariculture ponds.

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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A novel micro‐organism for removing excess ammonia‐N in seawater ponds and the effect of Cobetia amphilecti on the growth and immune parameters of Litopenaeus vannamei

Pan

Zhang

et al. 2018

J World Aquaculture Soc

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“…To date, several isolates belonging to Acinetobacter sp. have been reported to be capable of eliminating ammonia from both aquaculture wastewater and industrial effluents (Fan et al, 2015;Huang et al, 2013;Sarioglu et al, 2012;Zhao et al, 2010a), demonstrating the potential future use of this isolate for wastewater treatment.…”

Section: Isolation and Identification Of A Heterotrophic Nitrifying Bmentioning

confidence: 97%

Combination of heterotrophic nitrifying bacterium and duckweed (<i>Lemna gibba</i> L.) enhances ammonium nitrogen removal efficiency in aquaculture water via mutual growth promotion

Shen¹,

Yin

Xia

et al. 2019

J. Gen. Appl. Microbiol.

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To obtain high aquaculture output, up to 6,500 of fish or 10,000 shrimp are needed per 667 m 2 based on our investigations. As a result, high-protein feeds are needed in these aquatic systems. Urea, liquid cow manure, or even pig manure and chicken manure with high N content are often supplemented during this process (Lin and Yi, 2003; Moav et al., 1977; Soletto et al., 2005; Zoccarato et al., 1995). Budget-wise, about 87% of N comes from feed, while only 1% is released by denitrification (Acosta-Nassar et al., 2010). This results in the generation of substantial amounts of polluted effluent containing unconsumed feed and feces, and thus, leads to an increase in environmental pollution (Crab et al., 2007; Read and Fernandes, 2003). In these kinds of aquatic systems, levels of ammonia-N (NH 3-N), nitrite, and dissolved oxygen (DO) drastically affect aquaculture production (Crab et al., 2007; Zoccarato et al., 1995). Of these factors, NH 3-N is a critical concern; as it leads to an increase in nitrite and a decrease in DO due to the nitrification (Grommen et al., 2002; Kim et al., 2008; Ruiz et al., 2003). In addition, it is toxic for aquatic organisms (Romano and Zeng, 2013; Thompson et al., 2002). The presence of NH 3-N is inevitable, especially during intensive aquaculture, as they are generated from feed residues and manure supplements. Thus, there has been a lot of research trying to develop integrated pond systems using duckweed (Steen et al., 1999; Zimmo et al., 2003) or combined systems with other aquatic organisms such as algae (van der Steen et al., 1998), and cyanobacteria (Duong and Tiedje, 1985). Using the duckweed treatment system, not only NH 3-N, but also bacterial pathogens (El

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“…Nitrogen accumulation is one of the major causes of water pollution and eutrophication in aquaculture (Xia, Li & Wang 2008). The toxic nitrogen (ammonia and nitrite) is often excessive, which has heavily influence on the survival and growth of aquatic organisms and thus leads to huge economic loss in culture (Gross, Abutbul & Zilberg 2004;Fan, Chen, Liu, Wu, Meng & Song 2015). Nitrite is the intermediate of the conversion ammonia to nitrate.…”

Section: Introductionmentioning

confidence: 99%

Identification and real-time qPCR quantification of a nitrite-N degrading bacterial strain in aquatic water

Pan

et al. 2016

Aquac Res

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Biological nitrogen removal technology using microbe is an efficient process for nitrogen removal from aquatic water. To determine the key of efficient application on how the effect of probiotics lasting, a nitrite nitrogen (nitrite‐N) degrading 8DO17 strain Pseudomonas was screened and a method for quantification was explored. Real‐time qPCR assays based on the 16S rRNA genes (16S rDNA) were used to quantify the probiotics. The results showed that (i) the nitrite‐N degradation rate of the 8DO17 strain was 99%; (ii) a wide spectrum of pH (7.0–9.0) and concentration of nitrite‐N (0.5–10 mg L−1) and the highest rate of nitrite‐N degradation near to 100% under optimum conditions (35°C, salinity 30, pH 7.5) was explored; (iii) no significant differences were found in the survival, total haemocyte count, antibacterial activities and bacteriolytic activity of the shrimp between the treatments and the control (P > 0.05); (iv) for the use of real‐time PCR based on 16S rDNA test, detection limit is 103 DNA copies and an excellent correlation coefficients (R² = 0.999) was obtained; and (v) in the application of real‐time PCR assay in four nitrite‐N degrading samples, highly significant positive correlation relation (P < 0.01) was found between log copy number of 16S rDNA and the rate of nitrite‐N degradation. The results suggest the real‐time PCR is a very rapid and sensitive technique for monitoring the dynamic changes and assessing the effect in practical application of the 8DO17 strain in aquatic water.

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Exploration of three heterotrophic nitrifying strains from a tilapia pond for their characteristics of inorganic nitrogen use and application in aquaculture water

Cited by 11 publications

References 20 publications

A novel micro‐organism for removing excess ammonia‐N in seawater ponds and the effect of Cobetia amphilecti on the growth and immune parameters of Litopenaeus vannamei

A novel micro‐organism for removing excess ammonia‐N in seawater ponds and the effect of Cobetia amphilecti on the growth and immune parameters of Litopenaeus vannamei

Combination of heterotrophic nitrifying bacterium and duckweed (<i>Lemna gibba</i> L.) enhances ammonium nitrogen removal efficiency in aquaculture water via mutual growth promotion

Identification and real-time qPCR quantification of a nitrite-N degrading bacterial strain in aquatic water

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