Exploration of two-photon photostimulation for neuroscience

Kasai, Hideaki; Kanemoto, Yoshitsugu; Matsuzaki, Masunori; Ellis-Davis, Graham; Momotake, Atsuya; Arai, Tatsurou; Hahn, Klaus; Wu, Yuan-Hua; Ishikawa, Motoko; Noguchi, Junjirō; Hayama, Tatsuya; Watanabe, Satoshi; Morita, S.

doi:10.1016/j.neures.2009.09.1479

Cited by 1 publication

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“…[5][6][7][8] It relies on the drastic decrease of Ca 2+ affinity upon photolysis of the Ca 2+ -sequestrant. [9][10][11][12] Alternatively, electrochemistry could be a more attractive approach to switch the affinity via oxidation or reduction of the sequestrant. [13][14][15][16] The switching could be reversible and might occur with high spatial and temporal resolution thanks to the intrinsic properties of ultramicroelectrodes.…”

Section: Introductionmentioning

confidence: 99%

Water-soluble, redox-active organometallic calcium chelators

et al. 2012

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This paper describes a new series of organometallic water-soluble chelators combining a redox moiety (ferrocene) and a selective Ca2+ chelator (BAPTA) separated by an ethynyl bridge. We report the synthesis and characterization of organometallic derivatives of the BAPTA chelator featuring one (2a) and two ferrocenyl (2b) moieties. Single crystal X-ray structural analysis on these chelators revealed unexpected conformations for the ferrocenyl substituent with respect to the phenyl ring of the BAPTA unit. DFT calculations on a model system of the ferrocenyl-ethynyl-BAPTA molecule were carried out to evaluate the energy separation between the two limiting conformations observed experimentally in the solid state, and to check the effective electronic communication between the binding pocket and the redox probe. The binding affinity of 2a–b for Ca2+, as probed by UV-Vis and cyclic voltammetry, revealed distinct behaviors in the presence of a metal ion depending on whether BAPTA is substituted by one or two ferrocenyl groups.

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