Exploration of utility of combined optical photothermal infrared and Raman imaging for investigating the chemical composition of microcalcifications in breast cancer

Abstract

Microcalcifications play an important role in cancer detection. They are evaluated by their radiological and histological characteristics but it is challenging to find a link between their morphology, their composition...

Oleic acid differentially affectsde novolipogenesis in adipocytes and hepatocytes

“…The specific geometry also leads to significantly less signal contribution from water. 17,26 OPTIR has previously been used on fixed samples to obtain information on metabolic rates and chemical compositions, 27,28 but only recently has this technique been applied to live cell imaging. 17…”

“…The specific geometry also leads to significantly less signal contribution from water. 17,26 OPTIR has previously been used on fixed samples to obtain information on metabolic rates and chemical compositions, 27,28 but only recently has this technique been applied to live cell imaging. 17 We previously used OPTIR to track rates of DNL by feeding 13 C glucose to differentiated 3T3-L1 cells (adipocytes) and monitoring incorporation of the labelled carbon into the ester carbonyl of triglycerides in both live and fixed cells.…”

Spatially resolved multimodal vibrational spectroscopy under high pressures

“…Optical photothermal infrared and Raman spectroscopy bridges the gap between traditional infrared (far-field) spectroscopy and tip-based nano-IR spectroscopy, by providing a spatially resolved, and a non-contact method to probe infrared and Raman properties simultaneously, showing a broad range of applications in the areas of chemistry, biological and material sciences. 11–47 A schematic of this process (adapted from literature) is shown in Fig. 1(a).…”

“…The O-PTIR method also overcomes the limited pathlengths of conventional IR spectroscopy in water. 25 Though the O-PTIR technique is relatively new, it has proven useful in the studies of microplastics, 12,[18][19][20][41][42][43] polymer systems, 16,17 organic [18][19][20][21] and biological materials, [22][23][24][25][26][27][28][29][30][34][35][36][37][38] pharmaceuticals, 31,32 and even explosives. 33 Perhaps, most profoundly has been the influence of this spatially resolved system in the field of biology, where it has found success in everything from evaluating the biochemical structure of bone tissue 28 to studies of Alzheimer's disease (AD), and even exploration at the single cell.…”

Spectral effects and enhancement quantification in healthy human saliva with surface‐enhanced Raman spectroscopy using silver nanopillar substrates

“…Raman spectroscopy has been used for decades as a non-destructive tool for analysis of chemical, physical and biological analytes, and multiple research groups have explored spontaneous Raman spectroscopy (spontaneous RS) as a diagnostic method. 6,[12][13][14][15][16][17][18][19][20][21][22] Raman spectroscopy, coupled with machine learning (ML) techniques, can detect biomolecular features associated with vibrational modes of bonds from lipids, nucleic acids, proteins, as well as specific amino acids. 12,13,[23][24][25][26][27] Raman spectroscopy has been applied to biofluids for multiple detection purposes in dentistry, [28][29][30][31] forensics, [30][31][32][33][34][35] illicit drugs 30,31,[36][37][38] and healthcare.…”