Exploration of Wedelia chinensis leaf-assisted silver nanoparticles for antioxidant, antibacterial and in vitro cytotoxic applications

Abstract

Green synthetic route of silver nanoparticles (AgNPs) has already been proved to be an advantageous over other physico-chemical approaches due to its simplicity, cost effectiveness, ecofriendly and nontoxicity. In this finding, aqueous Wedelia chinensis leaf extract (WLE) mediated synthesis of AgNPs was approached. Surface plasmon resonance (SPR) band at 408 nm preliminary indicated the formation of AgNPs, while TEM and XRD characterization confirmed the formation of spherically shaped and crystalline AgNPs with an average size of 31.68 nm, respectively. The plausible biomolecules in the aqueous leaf extract responsible for the reduction and stabilization of AgNPs were identified by FTIR analysis and found to be polyphenolic groups in flavonoid. Further, synthesized AgNPs was explored for different biological applications. Biosynthesized AgNPs showed significant free radical scavenging activity as compared to Wedelia leaf extract and antibacterial activity against clinically isolated test pathogens where Gram-negative bacteria were found more susceptible to AgNPs than Gram-positive one. In addition, in vitro cytotoxic response was also evaluated on hepatocellular carcinoma Hep G2 cell lines and showed a dose-dependent cytotoxic response with an IC value of 25 μg/mL.

Green Synthesis of Controlled Shape Silver Nanostructures and Their Peroxidase, Catalytic Degradation, and Antibacterial Activity

“…Ag peaks are detected at 365–376 eV ( Figure 5 b,f). As a spin-orbital splitting, the Ag3d peak is shown as a doublet with two states: 367.5 eV Ag3d 5/2 and 373.5 eV Ag3d 3/2 with splitting energy of 6 eV, confirming the state in Ag 0 [ 52 , 53 ]. Figure 5 c describes the deconvolution of C1s spectra from AgNDs with four signals at 284.28, 285.5, 286.39, and 287.66 eV with respect to the C=C, C–O, C–N, and C=O relationships [ 54 , 55 , 56 ].…”

Allium sativum@AgNPs and Phyllanthus urinaria@AgNPs: a comparative analysis for antibacterial application

“…The results were in agreement with previous studies, in which the synergic effect of AgNPs and herbal extracts against various pathogenic bacteria was proved. 69–73 In term of particle size, the small size of AgNPs (4–5 nm) as well as the small size of Allium@AgNPs (13.5 nm) might contribute to high antibacterial property of the nanosystem. 74–76 Patra and Baek et al suggested that the AgNPs could strongly bind to the bacterial cell wall, then disrupt its structure.…”

Current trends in nano-delivery systems for functional foods: a systematic review

“…Nestlé’s low-fat ice cream uses nanoemulsions to achieve a low-fat content ( Sampathkumar, Tan & Say, 2020 ). These nanomaterials, as shown in several studies, enhance food packaging effectiveness, shelf life, and nutritional value without altering the flavor and physical properties of the food ( Bajpai et al, 2018 ; Paul Das et al, 2018 ). NDS has also been successfully used as processing aids, quality enhancers, antimicrobial agents, as well as in the creation of sensors for evaluating quality, safety and enhancement of the product’s physicochemical characteristics, nutrition and bioavailability ( Patra, Shin & Paramithiotis, 2018 ).…”