Exploration of α-pinene degradation pathway of Pseudomonas rhodesiae CIP 107491. Application to novalic acid production in a bioreactor

Linares, Denis; Fontanille, Pierre; Larroche, Christian

doi:10.1016/j.foodres.2008.12.001

Cited by 14 publications

(10 citation statements)

References 9 publications

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“…α-Pinene has high potential for human exposure since it was applied in various industries, such as pulp and paper industry, forest production, pharmaceutical industry, food industry, colour printing and paint process (Linares et al 2009;Montes et al 2010;Rene et al 2010;Wu et al 2012). In the current study, we evaluated the antiproliferative effects of α-pinene on both cultured primary rat neurons and N2a NB cells via the MTT assay for the first time.…”

Section: Discussionmentioning

confidence: 99%

Antioxidative, anticancer and genotoxic properties of α-pinene on N2a neuroblastoma cells

2013

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α-Pinene, an organic monoterpene, is found in essential oils of pine and coniferous trees. To date, although various biological activities of α-pinene have been demonstrated, its neurotoxicity has never been explored. Therefore in this study, we aimed to describe in vitro antiproliferative and/or cytotoxic properties by 3-(4,5-dimetylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test, genotoxic damage potentials by single cell gel electrophoresis, and oxidative effects by total antioxidant capacity (TAC) and total oxidative stress (TOS) analysis of α-pinene. Statistical analysis of MTT assay results indicated significant (p < 0.05) decreases of the cell proliferation rates in healthy neurons treated with α-pinene at only 400 mg/L, while significant decreases were observed in N2a cells at 100, 200 and 400 mg/L. On the other hand, the mean values of the total scores of cells showing DNA damage were not found significantly different from the control values on both cells. In addition, our results indicated that 10 and 25 mg/L of α-pinene treatment caused increases of TAC levels in primary rat neurons without any alterations of its level in N2a cells. However, α-pinene treatments at higher doses led to increases of TOS levels in both cell types. Overall our results suggest that α-pinene is of a limited therapeutic use as an anticancer agent.

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Section: Discussionmentioning

confidence: 99%

Antioxidative, anticancer and genotoxic properties of α-pinene on N2a neuroblastoma cells

2013

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“…Pseudomonas rhodesiae (CIP 107491) and P. fluorescens (NCIMB 11671) grew on α-pinene as sole carbon source. α-pinene is oxidized to α-pinene oxide [ 5 ] by a NADH-dependent α-pinene oxygenase (EC 1.14.12.155) and undergoes ring cleavage by action of a specific α-pinene oxide lyase (EC 5.5.1.10), forming apparently isonovalal as first product which is isomerized to novalal (Best et al, 1987 ; Bicas et al, 2008 ; Linares et al, 2009 ). The cleavage reaction of α-pinene oxide was also described for a Nocardia sp .…”

Section: Bicyclic Monoterpenesmentioning

confidence: 99%

Microbial monoterpene transformationsâ€”a review

2014

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Isoprene and monoterpenes constitute a significant fraction of new plant biomass. Emission rates into the atmosphere alone are estimated to be over 500 Tg per year. These natural hydrocarbons are mineralized annually in similar quantities. In the atmosphere, abiotic photochemical processes cause lifetimes of minutes to hours. Microorganisms encounter isoprene, monoterpenes, and other volatiles of plant origin while living in and on plants, in the soil and in aquatic habitats. Below toxic concentrations, the compounds can serve as carbon and energy source for aerobic and anaerobic microorganisms. Besides these catabolic reactions, transformations may occur as part of detoxification processes. Initial transformations of monoterpenes involve the introduction of functional groups, oxidation reactions, and molecular rearrangements catalyzed by various enzymes. Pseudomonas and Rhodococcus strains and members of the genera Castellaniella and Thauera have become model organisms for the elucidation of biochemical pathways. We review here the enzymes and their genes together with microorganisms known for a monoterpene metabolism, with a strong focus on microorganisms that are taxonomically validly described and currently available from culture collections. Metagenomes of microbiomes with a monoterpene-rich diet confirmed the ecological relevance of monoterpene metabolism and raised concerns on the quality of our insights based on the limited biochemical knowledge.

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“…Many previous studies have shown that Pseudomonas sp. has the ability to degrade α-pinene [32–36]. Therefore, Pseudomonas sp.…”

Section: Discussionmentioning

confidence: 99%

Effects of α-pinene on the pinewood nematode (Bursaphelenchus xylophilus) and its symbiotic bacteria

Wang

et al. 2019

PLoS ONE

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The pinewood nematode (PWN), Bursaphelenchus xylophilus , is an important plant-parasitic nematode that can cause severe mortality of pine trees. This PWN-induced harm to plants may be closely related to the abundance and diversity of the symbiotic microorganisms of the parasitic nematode. In this study, nematodes were divided into untreated and antibiotic-treated groups. Nematodes were treated by fumigation with different amounts of α-pinene, and the resultant mortality rates were analyzed statistically. Concentrations of symbiotic bacteria were calculated as colony-forming units per nematode. High-throughput sequencing was used to investigate the bacterial community structure. The results showed that the mortality of nematodes increased slightly with an increasing concentration of α-pinene, and nematodes untreated with antibiotics were more sensitive to α-pinene than those treated with antibiotics. The highest abundance of symbiotic bacteria was obtained via medium and low levels of α-pinene, but for which community diversity was the lowest (Shannon and Simpson indexes). The proportion of Pseudomonas spp. in the symbiotic bacteria of nematodes without antibiotics was relatively high (more than 70%), while that of Stenotrophomonas spp. was low (6%–20%). However, the proportion of Stenotrophomonas spp. was larger than that of Pseudomonas spp in the symbiotic bacteria associated with the antibiotic-treated nematodes. Pseudomonas sp. increased after pinene treatment, whereas Stenotrophomonas spp. decreased. These results indicate that although α-pinene has low toxicity to PWNs over a short time period, α-pinene ultimately influences the abundance and community diversity of the symbiotic bacteria of these nematodes; this influence may potentially disturb the development and reproduction of nematodes in the process of infecting pine trees.

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Exploration of α-pinene degradation pathway of Pseudomonas rhodesiae CIP 107491. Application to novalic acid production in a bioreactor

Cited by 14 publications

References 9 publications

Antioxidative, anticancer and genotoxic properties of α-pinene on N2a neuroblastoma cells

Antioxidative, anticancer and genotoxic properties of α-pinene on N2a neuroblastoma cells

Microbial monoterpene transformationsâ€”a review

Effects of α-pinene on the pinewood nematode (Bursaphelenchus xylophilus) and its symbiotic bacteria

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