Explore of the beneficial effects of Huang-Lian-Jie-Du Decoction on diabetic encephalopathy in db/db mice by UPLC-Q-Orbitrap HRMS/MS based untargeted metabolomics analysis

He, Wen-Jiao; Cao, Dong-Min; Chen, Yunbo; Shi, Juanjuan; Tian, Haijun; Zhang, Zhi-Tong; Lan, Tian; Tang, Dan; Wang, Shumei

doi:10.1016/j.jpba.2020.113652

Cited by 25 publications

(16 citation statements)

References 27 publications

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“…TCM can exert its effect by operating in the gut microbiota and is a natural intestinal microecological regulator, and the gut microbiota has emerged as a novel and important field for understanding TCM ( 22 , 23 ). Emerging evidence has indicated that the interactions between TCM and gut microbiota could lead to changes in structural or functional modulation of gut microbiota ( 24 , 25 ).…”

Section: Introductionmentioning

confidence: 99%

Danggui-Shaoyao-San Attenuates Cognitive Impairment via the Microbiota–Gut–Brain Axis With Regulation of Lipid Metabolism in Scopolamine-Induced Amnesia

et al. 2022

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Danggui-Shaoyao-San (DSS) has a long history of being used as a traditional medicine (TCM) and has been reported to show therapeutic effects in alleviating the symptoms of cognitive impairment. The purpose of this study was to investigate whether DSS treatment attenuates cognitive impairment via the microbiota–gut–brain axis in scopolamine-induced amnesia. In this work, we first performed the Morris water maze (MWM) test and novel object recognition (NOR) test to evaluate the memory function of treated C57BL/6N mice. Then we evaluated 16S rRNA for gut microbiota analysis, as well as assessment of blood–brain barrier function and intestinal barrier function and lipid metabolism analysis on tissues from different groups. We hypothesised that DSS may affect brain function and behavior through the gut–brain axis in a bidirectional interplay with both top-down and bottom-up regulation. Furthermore, in order to confirm whether intestinal flora plays a crucial role in scopolamine-induced amnesia, C57BL/6N mice were treated with fecal microbial transplantation (FMT), and then behavioral tests were performed. The mice’s feces were simultaneously evaluated by 16S rRNA analysis. The result supported that the FMT-induced improvement in cognitive function highlights the role of the gut microbiota–brain axis to mediate cognitive function and behavior. Besides theses works, more findings indicated that DSS altered lipid metabolism by activating LXR-PPAR-γ and repaired mucosal barrier dysfunction assessed with a broad range of techniques, which attenuated cognitive impairment via the microbiota–gut–brain axis.

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Section: Introductionmentioning

confidence: 99%

Danggui-Shaoyao-San Attenuates Cognitive Impairment via the Microbiota–Gut–Brain Axis With Regulation of Lipid Metabolism in Scopolamine-Induced Amnesia

et al. 2022

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“…In this study, the ten most common symptoms experienced by patients with T2DM were thirst and drinking more, blurred vision, mental fatigue, dry mouth and throat, insomnia and drowsiness, fear of heat, tiredness and weakness, sweating, frequent night urination, and cold pains in the waist and knees. He et al 11 indicated that the most common TCM syndromes were spleen-kidney yang deficiency and damp-heat phlegm stasis (accounting for 30.47% of the study population); qi-yin deficiency syndrome was found in another 69 patients (11.41%). Wang et al, 12 however, found that qi-yin deficiency occurred most frequently and that the frequency of damp-heat trapping spleen was not significant.…”

Section: Discussionmentioning

confidence: 98%

“…T2DM has become a major problem 11 in public health. Although developments in modern medical technology have facilitated a deeper understanding of T2DM, yin deficiency and excessive heat syndrome are unable to fully explain the pathogenesis of all T2DM cases.…”

Section: Discussionmentioning

confidence: 99%

The Distribution Pattern of Traditional Chinese Medicine Syndromes in 549 Patients with Type 2 Diabetes

Zhang¹,

Liu²,

Liang³

et al. 2021

DMSO

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Objective This study aimed to summarize the distribution pattern of traditional Chinese medicine (TCM) syndromes in patients with type 2 diabetes mellitus (T2DM). Methods The frequency, characteristics and distribution of all TCM syndromes of 549 patients with T2DM were analyzed. Results The average age of T2DM onset was higher in women than in men (ie, men experienced earlier onset). The distribution of TCM syndromes, in order of frequency, was as follows: damp-heat trapping spleen (including spleen deficiency and dampness, damp heat due to spleen deficiency, and qi weakness due to spleen deficiency) (58.29%), qi-yin deficiency (16.03%), deficiency of yin and excessive heat (12.93%), blood stasis in collaterals (9.41%), and yin-yang deficiency (3.21%). The physical intensity of patients’ occupational activity was mainly light (49.6%), followed by heavy (31.4%) and moderate (19.0%). Conclusion Damp-heat trapping spleen is the most common TCM syndrome in patients with T2DM, with damp heat due to spleen deficiency the most significant subtype. This syndrome tends to occur in people over the age of 60 and those undertaking too much or too little physical activity in their occupational activities. The traditional “three more and one less” symptoms do not adequately describe the clinical symptoms of T2DM.

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“…Metabolomics, a powerful qualitative and quantitative analysis for investigating the metabolic changes and regulations of small molecules in vivo, has been widely used in the discovery of disease biomarkers for DN . Ultra-performance liquid chromatography quadrupole/electrostatic field orbitrap coupled with high-resolution mass spectrometry (UPLC-Q-Orbitrap HRMS/MS) is an emerging mass spectrometry-based platform for metabolomics research. , However, the ionization efficiency of low-molecular-weight carbonyl compounds is poor, and it is also easily affected by the matrix in mass analysis. Thus, derivatization could be utilized for analyzing carbonyl compounds in complex matrices to improve mass ionization efficiency as well as chromatographic separation .…”

Section: Introductionmentioning

confidence: 99%

Metabolic Profiling of Carbonyl Compounds for Unveiling Protective Mechanisms of Pueraria lobata against Diabetic Nephropathy by UPLC-Q-Orbitrap HRMS/MS Analysis

Zhang

Guo

Zhuang

et al. 2021

J. Agric. Food Chem.

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Carbonyl compounds play a critical role in the pathogenesis of diabetic nephropathy (DN). Pueraria lobata (PL), also known as “Kudzu”, is a widely consumed functional food or nutraceutical and has shown promise in the prevention of diabetes and complications such as DN. To explore the beneficial effects and the underlying mechanisms of PL against DN, a new strategy for in-depth metabolic profiling of carbonyl compounds in DN mice plasma by chemical derivatization combined with UPLC-Q-Orbitrap high-resolution mass spectrometry (HRMS)/MS analysis was developed for the first time. Pharmacological evaluation revealed that PL extracts containing a total of 73 identified compounds could ameliorate kidney injury and regulate abnormal glycolipid metabolism. In metabolomics analysis, 19 carbonyl compounds with significant differences were identified between DN mice and normal mice. Moreover, 12 metabolites had a tendency to return to normal levels after PL treatment. Overall, PL exerts beneficial effects on DN by regulating abnormal glycolipid metabolism and carbonyl stress, and endogenous carbonyl compounds might serve as potential biomarkers for DN.

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Explore of the beneficial effects of Huang-Lian-Jie-Du Decoction on diabetic encephalopathy in db/db mice by UPLC-Q-Orbitrap HRMS/MS based untargeted metabolomics analysis

Cited by 25 publications

References 27 publications

Danggui-Shaoyao-San Attenuates Cognitive Impairment via the Microbiota–Gut–Brain Axis With Regulation of Lipid Metabolism in Scopolamine-Induced Amnesia

Danggui-Shaoyao-San Attenuates Cognitive Impairment via the Microbiota–Gut–Brain Axis With Regulation of Lipid Metabolism in Scopolamine-Induced Amnesia

The Distribution Pattern of Traditional Chinese Medicine Syndromes in 549 Patients with Type 2 Diabetes

Metabolic Profiling of Carbonyl Compounds for Unveiling Protective Mechanisms of Pueraria lobata against Diabetic Nephropathy by UPLC-Q-Orbitrap HRMS/MS Analysis

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