Exploring anti-MRSA activity of chitosan-coated liposomal dicloxacillin

Alshamsan, Aws; Aleanizy, Fadilah Sfouq; Badran, Mohamed M.; Alqahtani, Fulwah Yahya; Alfassam, Haya A.; Almalik, Abdulaziz; Alosaimy, Sara

doi:10.1016/j.mimet.2018.11.015

Cited by 29 publications

(25 citation statements)

References 35 publications

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“…A significantly wider zone of inhibition of dicloxacillin-loaded liposomes compared to free drug and drug-loaded chitosan-coated liposomes (55.0 ± 1.70, 34.3 ± 0.5, 33.0 ± 0.89 mm, respectively) confirmed the better antibacterial activity of small-sized liposomes as well as better drug biodistribution. Nevertheless, testing formulations in vivo on an MRSA infected animal model is recommended [42].…”

Section: -Better Protection and Enhanced Antibiotics Biodistributionmentioning

confidence: 99%

Nanomedicine Fight against Antibacterial Resistance: An Overview of the Recent Pharmaceutical Innovations

et al. 2020

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Based on the recent reports of World Health Organization, increased antibiotic resistance prevalence among bacteria represents the greatest challenge to human health. In addition, the poor solubility, stability, and side effects that lead to inefficiency of the current antibacterial therapy prompted the researchers to explore new innovative strategies to overcome such resilient microbes. Hence, novel antibiotic delivery systems are in high demand. Nanotechnology has attracted considerable interest due to their favored physicochemical properties, drug targeting efficiency, enhanced uptake, and biodistribution. The present review focuses on the recent applications of organic (liposomes, lipid-based nanoparticles, polymeric micelles, and polymeric nanoparticles), and inorganic (silver, silica, magnetic, zinc oxide (ZnO), cobalt, selenium, and cadmium) nanosystems in the domain of antibacterial delivery. We provide a concise description of the characteristics of each system that render it suitable as an antibacterial delivery agent. We also highlight the recent promising innovations used to overcome antibacterial resistance, including the use of lipid polymer nanoparticles, nonlamellar liquid crystalline nanoparticles, anti-microbial oligonucleotides, smart responsive materials, cationic peptides, and natural compounds. We further discuss the applications of antimicrobial photodynamic therapy, combination drug therapy, nano antibiotic strategy, and phage therapy, and their impact on evading antibacterial resistance. Finally, we report on the formulations that made their way towards clinical application.

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Section: -Better Protection and Enhanced Antibiotics Biodistributionmentioning

confidence: 99%

Nanomedicine Fight against Antibacterial Resistance: An Overview of the Recent Pharmaceutical Innovations

et al. 2020

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“…Since improper use of chemicals is often associated with the loss of lipoproteins and polysaccharides content, we purified OMVs without chemical treatment and confirmed their structural and morphological integrity [10,11,55,56]. As a polycationic polymer, CS is expected to form a positive coating around the negatively charged OMVs by electrostatic interaction which could be clearly evident from DLS data presented herein (Table 2) [57]. Expectedly, our biophysical analysis of CS coated OMVs suggest a marked increment in the mean hydrodynamic diameter of OMVs (149.9 to 221.9 nm) along with a significant drop in net negative charges (−23.4 to −8.2 mV).…”

Section: Discussionmentioning

confidence: 69%

Naturally secreted bacterial outer membrane vesicles: potential platform for a vaccine againstCampylobacter jejuni

Singh

Khan

Ghosh

et al. 2020

Preprint

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Acute diarrheal illness and gastroenteritis caused by Campylobacter jejuni ( C. jejuni ) infection remain significant public health risks in developing countries with substantial mortality and morbidity in humans, particularly in children under the age of five. Despite improved global awareness in sanitation and hygiene practices, including food safety measures, C. jejuni infections continue to rise even across the developed nations and no vaccine is currently available for humans. Genetic diversities among C. jejuni strains as well as limited understanding of immunological correlates of host protection remain primary impediments for developing an effective vaccine against C. jejuni . Given the role of bacterial outer membrane-associated proteins in intestinal adherence and invasion as well as modulating dynamic interplay between host and pathogens, bacterial outer membrane vesicles (OMVs) have emerged as potential vaccine platforms against a number of enteric pathogens, including C. jejuni . In the present study, we describe a mucosal vaccine strategy using chitosan (CS) coated OMVs (CS-OMVs) to induce specific immune responses against C. jejuni in mice. However, considering the challenges of mucosal delivery of OMVs in terms of exposure to variable pH, risk of enzymatic degradation, rapid gut transit, and low permeability across the intestinal epithelium, we preferentially used CS as a non-toxic, mucoadhesive polymer to coat OMVs. Mucosal administration of CS-OMVs induced high titre of systemic (IgG) and local (secretory IgA) antibodies in mice. The neutralizing ability of secretory IgA (sIgA) produced in the intestine was confirmed by in vitro inhibition of cell adherence and invasion of C. jejuni while in vivo challenge study in OMVs immunized mice showed a significant reduction in cecal colonization of C. jejuni . Moreover, to investigate the immunological correlates of the observed protection, present data suggest OMVs driven T cell proliferation with an increased population of CD4 + and CD8 + T cells. In addition to antibody isotype profile, significant upregulation of IFN-γ and IL-6 gene expression in mesenteric lymph nodes collected from OMVs immunized mice further suggests that mucosal delivery of OMVs promotes a Th1/Th2 mixed type immune responses. Together, we provide strong experimental evidence that as an acellular and non-replicating canonical end product of bacterial secretion, mucosal delivery of OMVs may represent a promising platform for developing an effective vaccine against C. jejuni .

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“…The coated vesicles have the ability to extend the residence time of the drug in circulation, leading to high targeting effect. Moreover, CS was exploited as a chaperone for liposomes to govern their surface characteristics with large loading capacity [15]. Nano-sized properties of CS-coated liposomes showed an excellent drug delivery with non-immunogenic characteristics due to their biological nature [13,15].…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, CS was exploited as a chaperone for liposomes to govern their surface characteristics with large loading capacity [15]. Nano-sized properties of CS-coated liposomes showed an excellent drug delivery with non-immunogenic characteristics due to their biological nature [13,15]. In this study, FLs were fabricated to increase drug loading and the flexibility of the membrane depending on their composition (Table 1 and Table 2).…”

Section: Resultsmentioning

confidence: 99%

“…CS is applied to improve muco-adhesiveness, which subsequently enhances drug absorption to promote sustained drug release [14]. CS is polycationic, and is used to form a positive layer around negatively charged liposomes via surface adsorption via electrostatic interaction [15]. This behavior of CS could enable efficient coating of phospholipid vesicles (chitosomes) in order to entrap drugs, leading to improved bioavailability [16].…”

Section: Introductionmentioning

confidence: 99%

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Chitosan-Coated Flexible Liposomes Magnify the Anticancer Activity and Bioavailability of Docetaxel: Impact on Composition

et al. 2019

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Flexible liposomes (FLs) were developed as promising nano-carriers for anticancer drugs. Coating them with chitosan (CS) could improve their drug delivery properties. The aim of this study was to investigate the physicochemical characteristics, pharmacokinetics behavior, and cytotoxic efficacy of docetaxel (DTX)-loaded CS-coated FLs (C-FLs). DTX-loaded FLs and C-FLs were produced via thin-film evaporation and electrostatic deposition methods, respectively. To explore their physicochemical characterization, the particle size, zeta potential, encapsulation efficiency (EE%), morphology, and DTX release profiles were determined. In addition, pharmacokinetic studies were performed, and cytotoxic effect was assessed using colon cancer cells (HT29). Various FLs, dependent on the type of surfactant, were formed with particle sizes in the nano-range, 137.6 ± 6.3 to 238.2 ± 14.2 nm, and an EE% of 59–94%. Moreover, the zeta potential shifted from a negative to a positive value for C-FL with increased particle size and EE%, and the in vitro sustained-release profiles of C-FL compared to those of FL were evident. The optimized C-FL containing sodium deoxycholate (NDC) and dicetyl phosphate (DP) elicited enhanced pharmacokinetic parameters and cytotoxic efficiency compared to those of the uncoated ones and Onkotaxel®. In conclusion, this approach offers a promising solution for DTX delivery.

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Exploring anti-MRSA activity of chitosan-coated liposomal dicloxacillin

Cited by 29 publications

References 35 publications

Nanomedicine Fight against Antibacterial Resistance: An Overview of the Recent Pharmaceutical Innovations

Nanomedicine Fight against Antibacterial Resistance: An Overview of the Recent Pharmaceutical Innovations

Naturally secreted bacterial outer membrane vesicles: potential platform for a vaccine againstCampylobacter jejuni

Chitosan-Coated Flexible Liposomes Magnify the Anticancer Activity and Bioavailability of Docetaxel: Impact on Composition

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