Exploring asthenozoospermia seminal plasma amino acid disorder based on GC-SIM-MS combined with chemometrics methods

Li, M. J.; Zhang, Z. M.; Fan, Fei; Ma, Ping; Wang, Y.; Lü, Hongmei

doi:10.1039/c9ay00134d

Cited by 10 publications

(8 citation statements)

References 47 publications

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“…The other most predominant amino acids revealed in our study were alanine, glycine, aspartic acid, and serine. When these amino acids in seminal plasma were compared to those found in human seminal plasma (Li et al, 2019), profiles of some seminal plasma amino acids were similar to those profiles we found such as serine, glycine, and glutamic acids. The least abundant in our study, on the other hand, are tyrosine, methionine, alphaaminobutyric acid, allo-isoleucine, and asparagine, and are found to be similar with the low levels of amino acids of methionine and tyrosine in bull (Assumpção et al, 2005).…”

Section: Discussionsupporting

confidence: 72%

Amino Acids of Seminal Plasma Associated With Freezability of Bull Sperm

Ugur

Hitit

Kaya

et al. 2020

Front. Cell Dev. Biol.

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Sperm cryopreservation is an important technique for fertility management, but postthaw viability of sperm differs among breeding bulls. With metabolites being the end products of various metabolic pathways, the contributions of seminal plasma metabolites to sperm cryopreservation are still unknown. These gaps in the knowledge base are concerning because they prevent advances in the fundamental science of cryobiology and improvement of bull fertility. The objective of this study was to test the hypothesis that seminal plasma amino acids are associated with freezability of bull sperm. To accomplish this objective, amino acid concentrations in seminal plasma from seven bulls of good freezability (GF) and six bulls of poor freezability (PF) were quantified using gas chromatography-mass spectrometry (GC-MS). Multivariate and univariate analyses were performed to identify potential freezability biomarkers. Pathways and networks analyses of identified amino acids were performed using bioinformatic tools. By analyzing and interpreting the results we demonstrated that glutamic acid was the most abundant amino acid in bull seminal plasma with average concentration of 3,366 ± 547.3 nM, which accounts for about 53% of total amino acids. The other most predominant amino acids were alanine, glycine, and aspartic acid with the mean concentrations of 1,053 ± 187.9, 429.8 ± 57.94, and 427 ± 101.3 nM. Pearson's correlation analysis suggested that phenylalanine concentration was significantly associated with post-thaw viability (r = 0.57, P-value = 0.043). Significant correlations were also found among other amino acids. In addition, partial least squares-discriminant analysis (PLS-DA) bi-plot indicated a distinct separation between GF and PF groups. Phenylalanine had the highest VIP score and was more abundant in the GF groups than in the PF groups. Moreover, pathway and network analysis indicated that phenylalanine contributes to oxidoreductase and antioxidant reactions. Although univariate analysis did not yield significant differences in amino acid concentration between the two groups, these findings are significant that they indicate the potentially important roles of amino acids in seminal plasma, thereby building a foundation for the fundamental science of cryobiology and reproductive biotechnology.

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Section: Discussionsupporting

confidence: 72%

Amino Acids of Seminal Plasma Associated With Freezability of Bull Sperm

Ugur

Hitit

Kaya

et al. 2020

Front. Cell Dev. Biol.

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“…In all, the quantification of amino acids in human plasma by both GC-MS-and LC-MS-based mass spectroscopy was well established [23,24,28,31,32] and would ideally suit our validation study after improvement. As the key differences for LC-MS were often in terms of sample preparation and separation parameters, the comparison of those published LC-MS methods with the UHPLC-MS/MS method established by us could find that (a) our analysis was time-saving and economical and without any derivatization process; (b) a unique chromatographic configuration minimized ion suppression and yielded excellent analytic performance, especially for the isomers; and (c) this method could easily be extended to different sample matrices, such as plasma (data not shown).…”

Section: Resultsmentioning

confidence: 99%

“…Many studies have published the quantitative analyses of amino acids in plasma [ 23 , 24 , 28 , 31 , 32 ], but these methods have never been applied in tissue homogenate. In this study, we developed a robust method for the quantitative analysis of underivatized amino acids in human tissue by UHPLC-MS/MS.…”

Section: Resultsmentioning

confidence: 99%

“…Amino acids, as a group of polar chemical compounds, should also be modified by using a derivatization reagent to make them volatile and thus accessible for GC-MS [ 27 ]. Over the past two decades, many researchers had described the ultrahigh-performance liquid chromatography-tandem mass spectrometry- (UHPLC-MS/MS-) based method for a targeted metabolomic study with increasing levels of sophistication [ 28 – 30 ]. Out of these considerations, thirty-four endogenous amino acids in human biological samples were involved in our study by using the LC-MS technique.…”

Section: Introductionmentioning

confidence: 99%

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Simultaneous Determination of 34 Amino Acids in Tumor Tissues from Colorectal Cancer Patients Based on the Targeted UHPLC-MS/MS Method

Yang

Zhang

Gao

et al. 2020

Journal of Analytical Methods in Chemistry

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A targeted ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was established and validated for the simultaneous determination of 34 amino acids in tissue samples from colorectal cancer (CRC) patients. The chromatographic separation was achieved on an Agilent ZORBAX SB-C18 column (3.0 × 150 mm, 5 μm) with a binary gradient elution system (A, 0.02% heptafluorobutyric acid and 0.2% formic acid in water, v/v; B, methanol). The run time was 10 min. The multiple reaction monitoring mode was chosen with an electrospray ionization source operating in the positive ionization mode for data acquisition. The linear correlation coefficients were >0.99 for all the analytes in their corresponding calibration ranges. The sample was pretreated based on tissue homogenate and protein precipitation with a 100 mg aliquot sample. The average recovery and matrix effect for 34 amino acids and 3 internal standards were 39.00%∼146.95% and 49.45%∼173.63%, respectively. The intra- and interday accuracy for all the analytes ranged from −13.52% to 14.21% (RSD ≤8.57%) and from −14.52% to 12.59% (RSD ≤10.31%), respectively. Deviations of stability under different conditions were within ±15% for all the analytes. This method was applied to simultaneous quantification of 34 amino acids in tissue samples from 94 CRC patients.

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“…The beneficial effect of valine on motility was previously reported in a study about the composition of free amino acids in sperm of several fish species, where motility of perch semen was positively influenced by the presence of valine ( Lahnsteiner, 2010 ). Regarding human, the exploration on seminal plasma amino acid disorder in patients with asthenozoospermia revealed significantly lower values of this amino acid than in healthy controls, pointing out valine as a potential biomarker closely related to the clinical parameters of this pathology ( Li et al., 2019 ). The mechanism by which valine exerts a beneficial effect on sperm motility is still unclear ( Lahnsteiner, 2010 ; Li et al., 2019 ); however, it has been observed that there is a direct effect of dietary supplemented valine on the antioxidant enzymes superoxide dismutase and glutathione peroxidase of hypercholesterolemic rats ( Cojocaru et al., 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Effect of supplementation of valine to chicken extender on sperm cryoresistance and post-thaw fertilization capacity

Bernal¹,

Iglesias-Cabeza²,

Sánchez-Rivera

et al. 2020

Poultry Science

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Recent reports showed a positive correlation between frozen–thawed rooster sperm DNA integrity and the concentrations of valine in seminal plasma. The present study evaluated the effect of supplementing valine to semen extender for freezing sperm of 2 endangered local Spanish chicken breeds with different sperm cryoresistance: Red Villafranquina ( VF ) showing low sperm DNA integrity after cryopreservation and Quail Castellana that shows higher DNA integrity. One pool of semen per breed was obtained twice a week for 10 wk (n = 40, 20 per breed). Each pool was divided into 2 fractions. One of these fractions was frozen in presence of valine as additive in the extender (concentration 10 mmol), whereas the other was used as control. The evaluation of the samples before and after freezing-thawing included motility (CASA-Mot system), viability (propidium iodide and SYBR-14), DNA integrity (terminal deoxynucleotidyl transferase dUTP nick end labeling), and fertility rate (percentage of eggs with blastoderm development after artificial insemination). Supplementation of valine increased several motility variables of fresh semen. In VF breed, valine increased percentage of progressive motile sperm ( P = 0.025), curvilinear velocity ( P = 0.033), straight-line velocity ( P = 0.040), and average path velocity ( P = 0.033), whereas progressive motile sperm ( P = 0.019), curvilinear velocity ( P = 0.006), straight-line velocity ( P = 0.003) and average path velocity ( P = 0.004) were improved in the Quail Castellana breed. Valine addition increased the DNA integrity of cryopreserved semen (decreased post-thaw DNA fragmentation) in both breeds, with a significant effect ( P = 0.002) in VF (36.3% VF-control vs 31%VF-valine). As expected, Quail Castellana cryopreserved sperm control showed higher fertility rate (34.4% ± 12.1) than VF cryopreserved sperm control (16.1% + 6.2). Supplementing valine to the freezing extender doubled ( P = 0.026) the fertility rate of VF (32.6% ± 12.2) compared with the control (16.1% + 6.2). In conclusion, supplementation of valine to chicken freezing extenders shows a positive effect on DNA fragmentation and fertilizing ability of frozen–thawed sperm, with a better response in a breed considered as the lowest freezer in our conservatory.

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Exploring asthenozoospermia seminal plasma amino acid disorder based on GC-SIM-MS combined with chemometrics methods

Abstract: Asthenozoospermia is a common cause of male infertility.

Cited by 10 publications

References 47 publications

Amino Acids of Seminal Plasma Associated With Freezability of Bull Sperm

Amino Acids of Seminal Plasma Associated With Freezability of Bull Sperm

Simultaneous Determination of 34 Amino Acids in Tumor Tissues from Colorectal Cancer Patients Based on the Targeted UHPLC-MS/MS Method

Effect of supplementation of valine to chicken extender on sperm cryoresistance and post-thaw fertilization capacity

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