Exploring bacterial diversity

Nazir, Ruqeya; Rehman, Sabeehah; Nisa, Marofull; Baba, Uqab ali

doi:10.1016/b978-0-12-817495-1.00007-4

Cited by 12 publications

(7 citation statements)

References 91 publications

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“…These bacteria are found in various environments and can survive extreme conditions. Some of them are known for their endospores production, which makes them resistant to desiccation (Parkes and Sass, 2009;Nazir et al, 2019). These bacteria, specially Bacillus spp., have been suggested to have a direct relationship with carbonate deposition and have been widely used for the consolidation and strengthening of sand columns and soils, the repairment of concrete cracks and in the field of limestone building conservation, showing in all cases a high capacity to precipitate calcite and vaterite (Le Métayer-Levrel et al, 1999;De Muynck et al, 2010García et al, 2016).…”

Section: After Treatmentmentioning

confidence: 99%

Bacterial Diversity Evolution in Maya Plaster and Stone Following a Bio-Conservation Treatment

et al. 2020

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Section: After Treatmentmentioning

confidence: 99%

Bacterial Diversity Evolution in Maya Plaster and Stone Following a Bio-Conservation Treatment

et al. 2020

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“…Besides, their combination with methicillin impaired slime formation of MRSA [90]. The slime layer is not easily be washed off and can be expressed as a virulence factor [91,92].…”

Section: Antibacterial Activities Of Cannabinoids Against Pathogens In the Who's Priority Listmentioning

confidence: 99%

Antimicrobial and Antiviral (SARS-CoV-2) Potential of Cannabinoids and Cannabis sativa: A Comprehensive Review

et al. 2021

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Antimicrobial resistance has emerged as a global health crisis and, therefore, new drug discovery is a paramount need. Cannabis sativa contains hundreds of chemical constituents produced by secondary metabolism, exerting outstanding antimicrobial, antiviral, and therapeutic properties. This paper comprehensively reviews the antimicrobial and antiviral (particularly against SARS-CoV-2) properties of C. sativa with the potential for new antibiotic drug and/or natural antimicrobial agents for industrial or agricultural use, and their therapeutic potential against the newly emerged coronavirus disease (COVID-19). Cannabis compounds have good potential as drug candidates for new antibiotics, even for some of the WHO’s current priority list of resistant pathogens. Recent studies revealed that cannabinoids seem to have stable conformations with the binding pocket of the Mpro enzyme of SARS-CoV-2, which has a pivotal role in viral replication and transcription. They are found to be suppressive of viral entry and viral activation by downregulating the ACE2 receptor and TMPRSS2 enzymes in the host cellular system. The therapeutic potential of cannabinoids as anti-inflammatory compounds is hypothesized for the treatment of COVID-19. However, more systemic investigations are warranted to establish the best efficacy and their toxic effects, followed by preclinical trials on a large number of participants.

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“…There are a number of papers speculating about and confirming the direct cytotoxicity of silver nanoparticles [ 4 , 5 , 8 , 9 , 10 ], but the genetic basis for the changes still remains unconfirmed. For a deeper explanation changes in the genes encoding OMP, flagella, fimbria, lipopolysaccharide (LPS), and exopolysaccharide by assigning the BLOSUM62 score [ 11 , 12 ], and based on an analysis of the mutfunc mutation database [ 13 ], has been showed. To our knowledge, this is the first publication where such a detailed analysis of the genome after bacteria treatment with selected SNF is shown.…”

Section: Introductionmentioning

confidence: 99%

How Bacteria Change after Exposure to Silver Nanoformulations: Analysis of the Genome and Outer Membrane Proteome

et al. 2021

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Objective: the main purpose of this work was to compare the genetic and phenotypic changes of E. coli treated with silver nanoformulations (E. coli BW25113 wt, E. coli BW25113 AgR, E. coli J53, E. coli ATCC 11229 wt, E. coli ATCC 11229 var. S2 and E. coli ATCC 11229 var. S7). Silver, as the metal with promising antibacterial properties, is currently widely used in medicine and the biomedical industry, in both ionic and nanoparticles forms. Silver nanoformulations are usually considered as one type of antibacterial agent, but their physical and chemical properties determine the way of interactions with the bacterial cell, the mode of action, and the bacterial cell response to silver. Methods: the changes in the bacterial genome, resulting from the treatment of bacteria with various silver nanoformulations, were verified by analyzing of genes (selected with mutfunc) and their conservative and non-conservative mutations selected with BLOSUM62. The phenotype was verified using an outer membrane proteome analysis (OMP isolation, 2-DE electrophoresis, and MS protein identification). Results: the variety of genetic and phenotypic changes in E. coli strains depends on the type of silver used for bacteria treatment. The most changes were identified in E. coli ATCC 11229 treated with silver nanoformulation signed as S2 (E. coli ATCC 11229 var. S2). We pinpointed 39 genes encoding proteins located in the outer membrane, 40 genes of their regulators, and 22 genes related to other outer membrane structures, such as flagellum, fimbria, lipopolysaccharide (LPS), or exopolysaccharide in this strain. Optical density of OmpC protein in E. coli electropherograms decreased after exposure to silver nanoformulation S7 (noticed in E. coli ATCC 11229 var. S7), and increased after treatment with the other silver nanoformulations (SNF) marked as S2 (noticed in E. coli ATCC 11229 var. S2). Increase of FliC protein optical density was identified in turn after Ag+ treatment (noticed in E.coli AgR). Conclusion: the results show that silver nanoformulations (SNF) exerts a selective pressure on bacteria causing both conservative and non-conservative mutations. The proteomic approach revealed that the levels of some proteins have changed after treatment with appropriate SNF.

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Exploring bacterial diversity

Cited by 12 publications

References 91 publications

Bacterial Diversity Evolution in Maya Plaster and Stone Following a Bio-Conservation Treatment

Bacterial Diversity Evolution in Maya Plaster and Stone Following a Bio-Conservation Treatment

Antimicrobial and Antiviral (SARS-CoV-2) Potential of Cannabinoids and Cannabis sativa: A Comprehensive Review

How Bacteria Change after Exposure to Silver Nanoformulations: Analysis of the Genome and Outer Membrane Proteome

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