Despite the presence of numerous navigable rivers in Brazil, they remain underutilized for Inland Waterway Transport (IWT). Given the recent changes in transport systems which aim to reduce reliance on highways due to their high cost and lack of sustainability, it becomes crucial to explore new transport models in Brazil, shifting part of the transport from roads to railways and mainly to waterways. To fill this gap in Brazil’s transport system, it was decided to check why waterways are little used and what challenges cause them to be underutilized and, additionally, what opportunities could leverage their use in the country. In this sense, in this study, we identify the biggest challenges and opportunities faced by IWT in Brazil. To achieve this objective, interviews were conducted using content analysis with managers who work in public and private IWT organizations active in Brazil. Results show that IWT has seen a recovery in interest in recent years due to the need for cheaper and greener logistics. It was also found that the main challenges that IWT faces are a lack of public policies followed by precarious infrastructure of waterways, ports and locks, as well as modest integration with other transport modes. Conversely, the most significant opportunity lies in the potential reduction in transport costs, coupled with the enhanced sustainability of transport activities via the utilization of IWT, thereby fostering greener transport practices. These results can contribute to a better understanding of practical and theoretical approaches related to IWT in Brazil, and they can serve as a reflection for new research focusing on the development of IWT especially in other emerging countries facing similar issues.