Several methods have been proposed for separation of eimerian oocysts and trichostrongylid eggs from extraneous debris; however, these methods have been considered to be still inconvenient in terms of time and wide-ranging applications. We describe herein an alternative way using the combination of electrical cream separator and vacuum filtration for harvesting and purifying eimerian oocysts and haemonchine eggs on large-scale applications with approximately 81% and 92% recovery rates for oocysts and nematode eggs obtained from avian and ovine faeces, correspondingly. The sporulation percentages as a measure of viability in the harvested oocysts and eggs from dry faecal materials are nearly 68% and 74%, respectively, and 12 liters of faecal suspension can be processed in approximately 7.5 min. The mode of separation in terms of costs (i.e. simple laboratory equipments and comparably cheap reagents) and benefits renders the reported procedure an appropriate pursuit to harvest and purify parasite oocysts and eggs on a large scale in the shortest duration from diverse volumes of environmental samples compared to the modified traditional sucrose gradient, which can be employed on a small scale.Many studies have been devoted to coccidiosis owing to significant economic losses to the poultry and many other domestic livestock industries throughout the world. Coccidiosis is a severe infection caused by the apicomplexan parasite of genus Eimeria in a host and predilection site specific manner 1-4 . Furthermore, among the gastrointestinal parasites that cause losses to the farming industry, for example, Ostertagia, Trichostrongylus, Nematodirus and Cooperia, Haemonchus contortus or the barber's pole worm is the predominant nematodes that infect small ruminants 5 .Coprologic examinations have long been conceded as an effective way for parasite identification that animal and human contracted with them. The most commonly employed technique in veterinary medicine, medical and clinical laboratory for separation of oocysts and eggs is the faecal floatation test. The technique depends on the differences in the specific gravity of the oocysts and eggs, the fluid floatation medium and debris. In other words, the differential density exists between them. Centrifugal flotation methods are clearly superior to non-centrifugation flotation procedures in terms of rapidity and efficiency.A considerable amount of literature has been published on eimerian oocyst and nematode egg separation from faecal materials. Techniques for the concentration and purification of oocysts and eggs from faecal samples include saturated salt solution flotation, sucrose density, zinc sulfate, and Percoll discontinuous density gradient centrifugation. Sucrose gradient ultracentrifugation has been exploited to separate subcellular fractions of Eimeria tenella sporozoites 6 . More recent attention has focused on the isolation of the endocytic organelle from macrophages by sucrose gradient 7 . In 1987, Arrowood and Sterling 8 published a paper in which they emplo...