Exploring interactions of copper hybrid surfactants with calf thymus-DNA

Bhar, Rekha; Kaur, Gurpreet; Mehta, S.K.

doi:10.1016/j.molliq.2017.06.054

Cited by 12 publications

(3 citation statements)

References 29 publications

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“…Hematite (α-Fe 2 O 3 ) NPs were synthesized hydrothermally from iron cetylpyridinium chloride II (FecpcII) precursor. Fecpc II was synthesized by the previously known method 36 . The pH of an aqueous solution of 20 mM Fecpc II was adjusted to 9.0 by NaOH.…”

Section: Methodsmentioning

confidence: 99%

Exploring drying pattern of a sessile droplet of genomic DNA in the presence of hematite nanoparticles

Bhar

Kaur

Mehta

2018

Sci Rep

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For the first time, drying pattern of a sessile droplet of genomic DNA, in the presence of hematite nanoparticles was sighted by polarizing optical microscopy (POM) in this research article. POM results indicated that only at an appreciably high concentration of hematite nanoparticles dried pattern of deoxyribonucleic acid from calf thymus (CT-DNA) was altered. Iron hybridized cetylpyridinium chloride was utilized for the preparation of iron oxide nanoparticles through hydrothermal method. Fourier transforms infrared spectroscopy (FTIR) and powder x-ray diffraction (PXRD) studies confirmed the formation of highly crystalline hematite i.e. α-Fe2O3 nanoparticles. Morphology of the synthesized nanoparticle was visualized by transmission electron microscope (TEM) and field emission scanning electron microscope (FESEM), which revealed that nanoparticles were rhombohedral in shape with a size of 45 ± 10 nm. Based upon all the findings, hydrothermal growth mechanism was also proposed having bilayer protection of surfactant around the nanoparticles. UV-Vis spectroscopy and fluorescence spectroscopy were explored to study the affinity of thus prepared nanoparticles towards calf thymus deoxyribonucleic acid (CT-DNA). The low value of binding constant calculated from the spectroscopy data confirmed the weak interaction between nanoparticles and the CT-DNA.

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Section: Methodsmentioning

confidence: 99%

Exploring drying pattern of a sessile droplet of genomic DNA in the presence of hematite nanoparticles

Bhar

Kaur

Mehta

2018

Sci Rep

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“…Anionic, nonionic and cationic surfactants have been investigated and, due to the charge complementarity, cationic surfactants have been shown to be by far the most efficient DNA compacting agents, inducing a morphological transition of the nucleic acid from an elongated form to a globular form [ 1 , 3 , 4 , 5 , 7 , 12 , 13 , 19 , 20 , 21 , 22 , 23 , 24 ]. An increment in the positive charge of the surfactant head group, or in the number of the positively charged head groups, tends, a priori, to increase the surfactant DNA compacting efficiency due to an increment in the electrostatic attractions between the negatively charged DNA and the surfactant [ 25 , 26 ]. The influence of the counter ion nature, the effect of substituting H atoms by F atoms in the hydrophobic chains, and the impact of the magnetic properties of the surfactants on the surfactant/DNA interactions, have also been investigated [ 27 , 28 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

“…An increase in hydrophobic chain length and the presence of additional side hydrophobic tails, results in an improved compacting efficiency [ 16 , 30 , 31 ]. More favorable hydrophobic interactions also explain that, for a given tail length, dimeric surfactants are more efficient DNA-condensing agents than single-chained surfactants [ 1 , 4 , 5 , 25 , 26 , 27 , 28 , 32 , 33 , 34 , 35 , 36 ]. Spacer length, which is a structural characteristic specific for oligomeric surfactants, also influences the surfactant/DNA interactions [ 37 , 38 , 39 , 40 , 41 ].…”

Section: Introductionmentioning

confidence: 99%

Cationic Single-Chained Surfactants with a Functional Group at the End of the Hydrophobic Tail DNA Compacting Efficiency

et al. 2021

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The interaction between calf-thymus DNA, ctDNA, and various single-chained surfactants with different functional groups at the end of hydrophobic tail was studied with the goal of investigating the influence of the functional group nature on surfactant DNA compacting efficiency. The surfactants investigated were dodecyltriethylammonium bromide (DTEABr), triethyl(1-phenoxydodecyl)ammonium bromide (12PhBr), triethyl(2-naphthoxydodecyl)ammonium bromide (12NBr) and 11-(isonicotinoyloxy)-N,N,N-triethyl-1-undecanaminium bromide (11PyBr). Results made evident that the surfactants’ tendencies to self-aggregation is the key factor determining their efficiency to compact the nucleic acid. Subsequently, DOPE/12NBr/pEGFP-C1 lipoplexes, with different cationic surfactant molar fractions (α) and mass ratios (L/D), were prepared and characterized. DOPE is a zwitterionic phospholipid 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine, and the plasmid pEGFP-C1 carries a GFP coding sequence with the necessary regulatory elements for constitutive expression of the gene in human cells. 12NBr was chosen because it was the most efficient DNA compacting agent among the surfactants investigated. Finally, the cytotoxicity and transfection efficiency (TE) of DOPE/12NBr/pDNA lipoplexes, with different compositions, were investigated.

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Supramolecular Metal‐Modified Nanocontainers Based on Amphiphilic and Hybrid Matrix: Self‐Assembling Behavior and Practical Applications

et al. 2022

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Exploring interactions of copper hybrid surfactants with calf thymus-DNA

Cited by 12 publications

References 29 publications

Exploring drying pattern of a sessile droplet of genomic DNA in the presence of hematite nanoparticles

Exploring drying pattern of a sessile droplet of genomic DNA in the presence of hematite nanoparticles

Cationic Single-Chained Surfactants with a Functional Group at the End of the Hydrophobic Tail DNA Compacting Efficiency

Supramolecular Metal‐Modified Nanocontainers Based on Amphiphilic and Hybrid Matrix: Self‐Assembling Behavior and Practical Applications

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