Exploring lipase-based biocatalysts derived from Yarrowia lipolytica for fatty acid ethyl esters synthesis

da Silva, Jéssica R.; Pedro, Kelly C. N. R.; da Silva, Gabrielle A. R.; Castro, Aline M.; Coelho, Maria Alice Z.; Ribeiro, Bernardo D.; Henriques, Cristiane A.; Langone, Marta A. P.

doi:10.1007/s43153-024-00492-6

Braz. J. Chem. Eng.

2024

DOI: 10.1007/s43153-024-00492-6

|View full text |Cite

Exploring lipase-based biocatalysts derived from Yarrowia lipolytica for fatty acid ethyl esters synthesis

Jéssica R. da Silva,

Kelly C. N. R. Pedro,

Gabrielle A. R. da Silva

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2024

Publication Types

Select...

Article1

Relationship

Self Cite0

Independent1

Authors

Journals

Cited by 1 publication

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Selection of Yarrowia lipolytica Lipases for Efficient Ester Synthesis or Hydrolysis

Souza,

Moura,

Franson

et al. 2024

Reactions

View full text Add to dashboard Cite

The species Yarrowia lipolytica is an aerobic yeast that produces different lipase isoforms, including extracellular, intracellular, and membrane-bound ones. The immobilization of lipases, such as those from Y. lipolytica, increases enzyme stability and lowers operational costs, through its reuse. The characterization of those biocatalysts is highly important to orientate their technological applications. The present work aims to obtain different Y. lipolytica lipases, through fermentation and immobilization techniques, and to evaluate the ester synthesis and hydrolysis activity of these biocatalysts in comparison to a commercial lipase produced by Candida rugosa and test them for phytosterol ester production. High immobilization yield was achieved by microencapsulating Y. lipolytica lipase extract on magnetic nanoparticles (>99.7%). However, immobilization significantly reduced their activity (more than 90%). Lipases from Y. lipolytica showed greater 4-nitrophenyl laurate synthesis in relation to the lipase from C. rugosa. However, C. rugosa lipase was still the best biocatalyst for β-sitosterol oleate synthesis, with a conversion of more than 99%. Y. lipolytica lipases can be good catalysts for ester hydrolysis reactions, even for ester synthesis, but are not good catalysts specifically for phytosterol esters synthesis.

show abstract

Selection of Yarrowia lipolytica Lipases for Efficient Ester Synthesis or Hydrolysis

Souza,

Moura,

Franson

et al. 2024

Reactions

View full text Add to dashboard Cite

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Exploring lipase-based biocatalysts derived from Yarrowia lipolytica for fatty acid ethyl esters synthesis

Cited by 1 publication

References 61 publications

Selection of Yarrowia lipolytica Lipases for Efficient Ester Synthesis or Hydrolysis

Selection of Yarrowia lipolytica Lipases for Efficient Ester Synthesis or Hydrolysis

Contact Info

Product

Resources

About