Exploring microfluidics as a tool to evaluate the biological properties of a titanium alloy under dynamic conditions

Carter, Sarah‐Sophia D.; Barbe, Laurent; Mestres, Gemma

doi:10.1039/d0bm00964d

Cited by 13 publications

(10 citation statements)

References 62 publications

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“…2 The protein and cellular adsorption behavior displayed in new and emergent approaches that may better reproduce the physiologic dynamic environment would be interesting to examine in the future. 29,39,40 Overall, this study proves the importance that the preincubation step in a serum-supplemented medium may have on biomaterials, whereby it enhances early cellular adhesion responses. Moreover, it was shown that computational modeling, in this case through the usage of two Langmuir isotherm equations that allowed coupling of protein-cell adhesion, enabled an accurate prediction of cell adhesion behavior over time.…”

Section: Discussionsupporting

confidence: 54%

“…In the current work, the cells were cultured under static conditions in standard well plates, although the environment created is known to poorly mimic the real physiological conditions . The protein and cellular adsorption behavior displayed in new and emergent approaches that may better reproduce the physiologic dynamic environment would be interesting to examine in the future. ,, …”

Section: Discussionmentioning

confidence: 99%

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Experimental Characterization and Mathematical Modeling of the Adsorption of Proteins and Cells on Biomimetic Hydroxyapatite

et al. 2021

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Biomaterial development is a long process consisting of multiple stages of design and evaluation within the context of both in vitro and in vivo testing. To streamline this process, mathematical and computational modeling displays potential as a tool for rapid biomaterial characterization, enabling the prediction of optimal physicochemical parameters. In this work, a Langmuir isotherm-based model was used to describe protein and cell adhesion on a biomimetic hydroxyapatite surface, both independently and in a one-way coupled system. The results indicated that increased protein surface coverage leads to improved cell adhesion and spread, with maximal protein coverage occurring within 48 h. In addition, the Langmuir model displayed a good fit with the experimental data. Overall, computational modeling is an exciting avenue that may lead to savings in terms of time and cost during the biomaterial development process.

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Section: Discussionsupporting

confidence: 54%

Section: Discussionmentioning

confidence: 99%

Experimental Characterization and Mathematical Modeling of the Adsorption of Proteins and Cells on Biomimetic Hydroxyapatite

et al. 2021

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“…OoC platforms can be used to study the biocompatibility and efficacy of biomaterials in an environ ment that is representative of the (patho)physiology of the organ or tissue to be replaced, repaired or regenerated. An important consideration is the integration of various biomaterials into the microfluidic OoC systems for fast and parallelized biocompatibility or biofunctionality testing 154,155 . Numerous different solutions for facile and versatile integration of biomaterials of various geometries 156,157 or bonding strategies have been reported 158 .…”

Section: Biomaterials Testingmentioning

confidence: 99%

A guide to the organ-on-a-chip

Leung

Haan

Ronaldson-Bouchard

et al. 2022

Nat Rev Methods Primers

509

276

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The organ-on-a-chip (OoC) is an intriguing scientific and technological development in which biology is coupled with microtechnology 1,2 to mimic key aspects of human physiology. The chip takes the form of a microfluidic device containing networks of hair-fine microchannels for guiding and manipulating minute volumes (picolitres up to millilitres) of solution [3][4][5] . The organ is a more relatable term that refers to the miniature tissues grown and residing in the microfluidic chips, which can recapitulate one or more tissue-specific functions. Although they are much simpler than native tissues and organs, scientists have discovered that these systems can often serve as effective mimics of human physiology and disease. OoCs comprise advanced in vitro technology that enables experimentation with biological cells and tissues outside the body. This is achieved by containing them inside vessels conditioned to sustain a reasonable semblance of the in vivo environment, from a biochemical and physical point of view. Working on the microscale lends a unique opportunity to attain a higher level of control over the microenvironment that ensures tissue life support, as well as a means to directly observe cell and tissue behaviour.The OoC is a relatively recent addition to the toolbox of model biological systems available to life science researchers to probe aspects of human pathophysiology and disease. These systems cover a spectrum of physiological relevance, with 2D cell cultures the least relevant, followed in increasing order by 3D cell cultures, organoids and OoCs. Unsurprisingly, the use of model organisms such as mice and Drosophila physiologically exceeds engineered tissue approaches 6,7 . While biological complexity increases with physiological relevance in model organisms, this unfortunately leads to increased experimental difficulty. In vivo physiological processes are, in many ways, the least accessible to direct investigation in mice, humans and other mammals, despite significant advances in in vivo imaging. However, 2D and 3D cell cultures, such as spheroids and stem cell-derived organoids, sacrifice some aspects of in vivo relevance to facilitate experimentation. The OoC may be regarded as a bridging technology, offering the ability to work with complex cell cultures, while providing better engineered microenvironments to maximize the model.Following on from early concepts, including animal-on-a-chip 8 , body-on-a-chip 9 and breathing lung-on-a-chip 10 , research in the OoC and microphysiological systems fields has grown exponentially; evidenced by numerous excellent reviews published recently 1,2,11 . Recognition of OoC technology now extends far beyond university laboratories, driven by a need to better understand the human physiology underlying health and disease, and to find new approaches to improve the human condition. The World Economic Forum, for instance, selected the OoC as one of the top ten emerging technologies in 2016 (ref. 12

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“…Taken together, these results demonstrate that hBCSFB-on-chip recreates some physiologically-relevant functions, which are also in line with previous studies showing that dynamic flow can improve the biological properties of cells. 34,37…”

Section: Hbcsfb-on-chip Recapitulates the Physiologically-relevant Tr...mentioning

confidence: 99%

Bioengineering of a human physiologically relevant microfluidic blood–cerebrospinal fluid barrier model

Zhou

Qiao

et al. 2023

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Exploring microfluidics as a tool to evaluate the biological properties of a titanium alloy under dynamic conditions

Abstract: To bring novel biomaterials to clinical use, reliable in vitro models are imperative. The aim of this work was to develop a microfluidic tool to evaluate the biological properties of...

Cited by 13 publications

References 62 publications

Experimental Characterization and Mathematical Modeling of the Adsorption of Proteins and Cells on Biomimetic Hydroxyapatite

Experimental Characterization and Mathematical Modeling of the Adsorption of Proteins and Cells on Biomimetic Hydroxyapatite

A guide to the organ-on-a-chip

Bioengineering of a human physiologically relevant microfluidic blood–cerebrospinal fluid barrier model

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