Exploring Molecular Interactions between Human Milk Hormone Insulin and Bifidobacteria

Rizzo, Sonia Mirjam; Alessandri, Giulia; Lugli, Gabriele Andrea; Fontana, Federico; Tarracchini, Chiara; Mancabelli, Leonardo; Viappiani, Alice; Bianchi, Massimiliano; Bussolati, Ovidio; Sinderen, Douwe van; Ventura, Marco; Turroni, Francesca

doi:10.1128/spectrum.00665-23

Cited by 6 publications

(2 citation statements)

References 58 publications

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“…To support PRL2012 strain resilience in the human gut, its adhesive performances to human intestinal mucosal cells were evaluated using a previously described methodology (Guglielmetti et al, 2008 ; Turroni et al, 2013 ; Rizzo et al, 2023 ), seeding cells of B. breve PRL2012 on human colon intestinal HT29-MTX monolayers. After multiple washes with PBS, the number of microbial cells attached to the HT29-MTX monolayer was assessed in order to calculate the adhesion index, which represents the number of bacterial cells adhering to 100 HT29-MTX cells (Guglielmetti et al, 2008 ).…”

Section: Resultsmentioning

confidence: 99%

“…The proportion of bacterial cells that remained attached to the HT29-MTX monolayer was determined to reflect the extent of specific host-microbe interaction. The adhesion index represents the average number of bacterial cells attached to 100 HT29-MTX cells (Guglielmetti et al, 2008 ; Turroni et al, 2013 ; Rizzo et al, 2023 ). A non-parametric Mann Whitney test was applied for the detection of statistically significant differences.…”

Section: Methodsmentioning

confidence: 99%

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Genomic and ecological approaches to identify the Bifidobacterium breve prototype of the healthy human gut microbiota

Argentini,

Lugli,

Tarracchini

et al. 2024

Front. Microbiol.

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Members of the genus Bifidobacterium are among the first microorganisms colonizing the human gut. Among these species, strains of Bifidobacterium breve are known to be commonly transmitted from mother to her newborn, while this species has also been linked with activities supporting human wellbeing. In the current study, an in silico approach, guided by ecology- and phylogenome-based analyses, was employed to identify a representative strain of B. breve to be exploited as a novel health-promoting candidate. The selected strain, i.e., B. breve PRL2012, was found to well represent the genetic content and functional genomic features of the B. breve taxon. We evaluated the ability of PRL2012 to survive in the gastrointestinal tract and to interact with other human gut commensal microbes. When co-cultivated with various human gut commensals, B. breve PRL2012 revealed an enhancement of its metabolic activity coupled with the activation of cellular defense mechanisms to apparently improve its survivability in a simulated ecosystem resembling the human microbiome.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Genomic and ecological approaches to identify the Bifidobacterium breve prototype of the healthy human gut microbiota

Argentini,

Lugli,

Tarracchini

et al. 2024

Front. Microbiol.

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Gut-mammary pathway: Breast milk microbiota as a mediator of maternal gut microbiota transfer to the infant gut

Meng,

Xie,

et al. 2025

Journal of Functional Foods

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GH136‐encoding gene (perB) is involved in gut colonization and persistence by Bifidobacterium bifidumPRL2010

Rizzo,

Vergna,

Alessandri

et al. 2024

Microbial Biotechnology

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Bifidobacteria are commensal microorganisms that typically inhabit the mammalian gut, including that of humans. As they may be vertically transmitted, they commonly colonize the human intestine from the very first day following birth and may persist until adulthood and old age, although generally at a reduced relative abundance and prevalence compared to infancy. The ability of bifidobacteria to persist in the human intestinal environment has been attributed to genes involved in adhesion to epithelial cells and the encoding of complex carbohydrate‐degrading enzymes. Recently, a putative mucin‐degrading glycosyl hydrolase belonging to the GH136 family and encoded by the perB gene has been implicated in gut persistence of certain bifidobacterial strains. In the current study, to better characterize the function of this gene, a comparative genomic analysis was performed, revealing the presence of perB homologues in just eight bifidobacterial species known to colonize the human gut, including Bifidobacterium bifidum and Bifidobacterium longum subsp. longum strains, or in non‐human primates. Mucin‐mediated growth and adhesion to human intestinal cells, in addition to a rodent model colonization assay, were performed using B. bifidum PRL2010 as a perB prototype and its isogenic perB‐insertion mutant. These results demonstrate that perB inactivation reduces the ability of B. bifidum PRL2010 to grow on and adhere to mucin, as well as to persist in the rodent gut niche. These results corroborate the notion that the perB gene is one of the genetic determinants involved in the persistence of B. bifidum PRL2010 in the human gut.

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Exploring Molecular Interactions between Human Milk Hormone Insulin and Bifidobacteria

Cited by 6 publications

References 58 publications

Genomic and ecological approaches to identify the Bifidobacterium breve prototype of the healthy human gut microbiota

Genomic and ecological approaches to identify the Bifidobacterium breve prototype of the healthy human gut microbiota

Gut-mammary pathway: Breast milk microbiota as a mediator of maternal gut microbiota transfer to the infant gut

GH136‐encoding gene (perB) is involved in gut colonization and persistence by Bifidobacterium bifidumPRL2010

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