Exploring molecular variation in Schistosoma japonicum in China

Young, Neil D.; Chan, Kok‐Gan; Korhonen, Pasi K.; Chong, Teik Min; Ee, Robson; Mohandas, Namitha; Koehler, Anson V.; Lim, Yan-Lue; Hofmann, Andreas; Jex, Aaron R.; Bao-zhen, Qian; Chilton, Neil B.; Gobert, Geoffrey N.; McManus, Donald P.; Tan, Patrick; Webster, Bonnie L.; Rollinson, David; Gasser, Robin B.

doi:10.1038/srep17345

Cited by 36 publications

(17 citation statements)

References 102 publications

(171 reference statements)

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“…The ability to study genetic diversity in populations on a genome-wide scale, and to associate variation to genes or proteins linked to important biological characteristics, could have important implications for the control of myiasis. Recently, a practical and cost-effective high throughput genome-wide sequencing-mapping-phylogenetic approach was established to investigate genetic diversity among populations of a parasitic blood fluke (Schistosoma japonicum) of major socioeconomic importance (Young et al, 2015). This approach should be applicable to a wide range of eukaryotic pathogens, including flies, and provides a tool to evaluate sequence variation in any exon of any single-copy genes associated with particular phenotypic traits, such as pathogenicity, drug susceptibility/resistance or immunogenicity, and to assess how a particular intervention might relate to, or affect, the phenotype and genotype of L. cuprina.…”

Section: Discussionmentioning

confidence: 99%

A blow to the fly — Lucilia cuprina draft genome and transcriptome to support advances in biology and biotechnology

Anstead

Batterham

Korhonen

et al. 2016

Biotechnology Advances

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The blow fly, Lucilia cuprina (Wiedemann, 1830) is a parasitic insect of major global economic importance. Maggots of this fly parasitize the skin of animal hosts, feed on excretions and tissues, and cause severe disease (flystrike or myiasis). Although there has been considerable research on L. cuprina over the years, little is understood about the molecular biology, biochemistry and genetics of this parasitic fly, as well as its relationship with its hosts and the disease that it causes. This situation might change with the recent report of the draft genome and transcriptome of this blow fly, which has given new and global insights into its biology, interactions with the host animal and aspects of insecticide resistance at the molecular level. This genomic resource will likely enable many fundamental and applied research areas in the future. The present article gives a background on L. cuprina and myiasis, a brief account of past and current treatment, prevention and control approaches, and provides a perspective on the impact that the L. cuprina genome should have on future research of this and related parasitic flies, and the design of new and improved interventions for myiasis.

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Section: Discussionmentioning

confidence: 99%

A blow to the fly — Lucilia cuprina draft genome and transcriptome to support advances in biology and biotechnology

Anstead

Batterham

Korhonen

et al. 2016

Biotechnology Advances

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“…Although the extent of variability within C. sinensis appears to be low, there have been no comprehensive studies to date to explore population genetic variation among relatively large numbers of isolates from disparate geographic locations using complete or near complete mt and/or nuclear genomic data sets. Nonetheless, such studies have been conducted on other flatworm parasites, such as the blood flukes Schistosoma japonicum and S. mansoni, using advanced deep sequencing and bioinformatics (Young et al, 2015;Crellen et al, 2016). In the present study, our goal was to assess and apply a deep sequencing-bioinformatic approach to sequence and define a reference mt genome for a particular isolate of C. sinensis from Korea, in order to confirm the specific identity of the parasite, and to compare this reference mt genome to all mt genomic data sets currently available for this and selected species of trematodes, as a basis for a nuclear genomic sequencing project and a future global analysis of genetic variation within and among isolates from parts of Southeast Asia.…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial genomic comparison of Clonorchis sinensis from South Korea with other isolates of this species

Wang

Young

Koehler

et al. 2017

Infection, Genetics and Evolution

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Clonorchiasis is a neglected tropical disease that affects >35 million people mainly in China, Vietnam, South Korea and some parts of Russia. The disease-causing agent, Clonorchis sinensis, is a liver fluke of humans and other piscivorous animals, and has a complex aquatic life cycle involving snails and fish intermediate hosts. Chronic infection in humans causes liver disease and associated complications including malignant bile duct cancer. Central to control and to understanding the epidemiology of this disease is knowledge of the specific identity of the causative agent as well as genetic variation within and among populations of this parasite. Although most published molecular studies seem to suggest that C. sinensis represents a single species and that genetic variation within the species is limited, karyotypic variation within C. sinensis among China, Korea (2n=56) and Russian Far East (2n=14) suggests that this taxon might contain sibling species. Here, we assessed and applied a deep sequencing-bioinformatic approach to sequence and define a reference mitochondrial (mt) genome for a particular isolate of C. sinensis from Korea (Cs-k2), to confirm its specific identity, and compared this mt genome with homologous data sets available for this species. Comparative analyses revealed consistency in the number and structure of genes as well as in the lengths of protein-coding genes, and limited genetic variation among isolates of C. sinensis. Phylogenetic analyses of amino acid sequences predicted from mt genes showed that representatives of C. sinensis clustered together, with absolute nodal support, to the exclusion of other liver fluke representatives, but sub-structuring within C. sinensis was not well supported. The plan now is to proceed with the sequencing, assembly and annotation of a high quality draft nuclear genome of this defined isolate (Cs-k2) as a basis for a detailed investigation of molecular variation within C. sinensis from disparate geographical locations in parts of Asia and to prospect for cryptic species.

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“…In addition, the same effect may be achieved by adding different perspectives of investigation, such as epigenetic analysis or protein modification. On the other hand, considerable variation between some of S. japonicum populations has been reported for genes inferred to be linked to key cellular processes and/or pathogen-host interactions [43]. In our study, we only used one population in the infection experiment which showed no significant differences.…”

Section: Discussionmentioning

confidence: 94%

Oncomelania hupensis retains its ability to transmit Schistosoma japonicum 13 years after migration from permissive to non-permissive areas

et al. 2020

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Background: The East Route Project (ERP) of the South-to-North Water Diversion Project (SNWDP) stretches across schistosomiasis endemic and non-endemic areas in China, which may lead to the dispersal of Oncomelania hupensis, the intermediate host of Schistosoma japonicum, from permissive areas along the Yangtze River Basin to non-permissive areas in northern China. A previous survey demonstrated that O. hupensis could survive and breed for 13 years (12 generations) after being transferred to a non-permissive area, and could be infected by S. japonicum. However, it is not clear if the migrated snails will change their ability to transmit S. japonicum. Methods: We infected mice with the cercariae released from the infected transferred snails bred in Jining city of Shandong Province (non-permissive areas) for 13 years. The mice in the control group were infected with cercariae derived from the snails collected in their original habitat (Jiangdu county of Jiangsu Province, permissive areas). Then, we explored the pathogenicity to mice including worm burden, liver egg count and pathology. Additionally, the gene expression profiles of the adult male and female worms recovered from the infected mice were analyzed by RNA sequencing. Results: The worm burden, liver egg count and pathology of the mice infected with cercariae released from transferred snails bred in non-permissive areas for 13 years showed no significant differences, when compared with the control cercariae. Slight changes occurred at the transcription level between adult male and female worms recovered from mice infected with cercariae derived from snails bred in permissive and non-permissive areas. Only fourteen genes were significantly differentially expressed in the comparison of adult female worms, and no significantly differentially expressed gene was found in the comparison of adult male worms. Conclusions: Our findings strongly suggest that transferred snails did not change their schistosomiasis transmission ability and the worms derived from them retained the original pathogenicity, even after migrating from permissive to non-permissive areas for 13 years. Therefore, a long-term surveillance system of snails along the SNWDP is urgently needed to prevent the diffusion of O. hupensis and reduce the risk of transmission of schistosomiasis.

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Exploring molecular variation in Schistosoma japonicum in China

Cited by 36 publications

References 102 publications

A blow to the fly — Lucilia cuprina draft genome and transcriptome to support advances in biology and biotechnology

A blow to the fly — Lucilia cuprina draft genome and transcriptome to support advances in biology and biotechnology

Mitochondrial genomic comparison of Clonorchis sinensis from South Korea with other isolates of this species

Oncomelania hupensis retains its ability to transmit Schistosoma japonicum 13 years after migration from permissive to non-permissive areas

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