Exploring species diversity of lissorchiid trematodes (Digenea: Lissorchiidae) associated with the gravel snail,<i>Lithoglyphus naticoides</i>, in European freshwaters

Petkevičiūtė, Romualda; Stanevičiūtė, Gražina; Stunžėnas, Virmantas

doi:10.1017/s0022149x2000036x

Cited by 10 publications

(15 citation statements)

References 39 publications

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“…Phylogenetic data support the unification of the lissorchiine and the asymphylodorine digeneans into the same family but do not support the monophyly of the Lissorchiinae ( [9][10][11]40], Present Study). Notably, the position of two lissorchiine genera, Lissorchis and Palaeorchis Szidat, 1943, is unstable in the phylograms of different authors [10,11], Present Study. Anarhichotrema, in contrast to the other lissorchiid genera, has a marine life history and the filamented eggs compare with [5,9,11].…”

Section: Discussioncontrasting

confidence: 58%

“…The division is based on the number of testes: two in the former and one in the latter subfamily [5]. Phylogenetic data support the unification of the lissorchiine and the asymphylodorine digeneans into the same family but do not support the monophyly of the Lissorchiinae ( [9][10][11]40], Present Study). Notably, the position of two lissorchiine genera, Lissorchis and Palaeorchis Szidat, 1943, is unstable in the phylograms of different authors [10,11], Present Study.…”

Section: Discussionmentioning

confidence: 75%

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Phylogenetic Evidence for the Lissorchiid Concept of the Genus Anarhichotrema Shimazu, 1973 (Trematoda, Digenea)

2022

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Anarhichotrema Shimazu, 1973 is a monotypic digenean genus, with the type- and only species, Anarhichotrema ochotense Shimazu, 1973, known to infect North Pacific fishes. This genus was originally described as a member of the Lissorchiidae (Monorchioidea) and later moved to the Zoogonidae (Microphalloidea). Its exact phylogenetic position has remained unresolved due to the lack of molecular data. In this study, we isolated specimens of A. ochotense from the Bering wolffish, Anarhichas orientalis Pallas, 1814 caught in the Sea of Okhotsk, described them morphologically and performed a molecular phylogenetic analysis of their nuclear 18S and 28S rDNA regions. The specimens examined in our study generally corresponded to previous morphological descriptions of A. ochotense but were noticeably smaller, possibly due to the crowding effect. The phylogenetic analysis placed Anarhichotrema within the Lissorchiidae as a sister taxon to the group comprising freshwater lissorchiids. Thus, we restore Anarhichotrema to the Lissorchiidae, as originally assigned.

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Section: Discussioncontrasting

confidence: 58%

Section: Discussionmentioning

confidence: 75%

Phylogenetic Evidence for the Lissorchiid Concept of the Genus Anarhichotrema Shimazu, 1973 (Trematoda, Digenea)

2022

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“…Genomic DNA was isolated from ethanol-fixed specimens according to Stunžėnas' protocol (Stunžėnas et al, 2011;Petkevičiūtė et al, 2014). Amplification of the two rDNA regions was performed following the protocol used in our previous studies (Petkevičiūtė et al, 2014(Petkevičiūtė et al, , 2020. The ITS2 was amplified using the forward primer 3S (5 ′ -CGG TGG ATC ACT CGG CTC GTG-3 ′ ) (Bowles et al, 1995) and the reverse primer ITS2.2 (5 ′ -CCT GGT TAG TTT CTT TTC CTC CGC-3 ′ ) (Cribb et al, 1998).…”

Section: Molecular Sequencing and Phylogenetic Analysesmentioning

confidence: 99%

“…The clarification of phylogenetic relationships of trematodes today undoubtedly depends on molecular markers. Unfortunately, a comprehensive molecular phylogenetic analysis of relationships between lissorchiids is lacking, as only a few species were available for previous studies (Besprozvannykh et al, 2012; Sokolov & Gordeev, 2019; Petkevičiūtė et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Diversity of European lissorchiid trematodes from fish and snail hosts with comments on the validity of the genusParasymphylodoraSzidat, 1943

Petkevičiūtė

Stunžėnas²,

Stanevičiūtė³

2022

J. Helminthol.

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Genetic markers, DNA sequences and karyotypes, of some European lissorchiid species from their intermediate and final hosts were obtained to clarify controversial data about their life cycles and taxonomy, and to reveal phylogenetic affinities. The life cycles of three species have been confirmed for the first time based on molecular data. Comparative analysis of internal transcribed spacer 2 (ITS2) and partial 28S rDNA sequences has undoubtedly proven that cercariaeum of type-species of the genus Asymphylodora, Asymphylodora tincae, develops in pulmonate snails, Anisus vortex and Stagnicola palustris, but not in the genus Bithynia. The faucet snail, Bithynia tentaculata, serves as the first intermediate host for Parasymphylodora (=Asymphylodora) markewitschi and Parasymphylodora parasquamosa; adults of both species were isolated from the common rudd, Scardinius erythrophthalmus. It has also been confirmed that B. tentaculata serves as the second intermediate host for P. parasquamosa. Phylogenetic analysis supports the validity of the genus Parasymphylodora. Two species, Parasymphylodora markewitschi and P. parasquamosa, with cercariaeum belonging to the Parasquamosum group, are closely related and are being recovered as a well-defined evolutionary lineage in phylogenetic trees. A significant divergence between Parasymphylodora spp. and Asymphylodora spp. was revealed. The diploid chromosome set of P. markewitschi is composed of 14 chromosomes and does not show similarities with karyotypes of other lissorchiid species. Asymphylodora progenetica and Asymphylodora tincae share the basal diploid value of the family, 2n = 20, and reveal very close morphology of the corresponding chromosome pairs. Karyotypic similarities of these species are in accordance with molecular phylogenetic data. Thus, the available molecular and cytogenetic data support the assignment of P. markewitschi and P. parasquamosa to a separate genus, meanwhile, the assignment of A. progenetica to the genus Parasymphylodora was not justified.

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“…recorded a cercaria of this type from B. funiculata in Thailand. Other previous reports of lissorchiid cercariae from bithyniid species includePetkevičiūtė et al (2020) andSchwelm et al (2020), both from Europe, andBesprozvannykh et al (2012) in the Russian Far East.Wiroonpan et al (2021) reported mutabile cercariae to be common in B. s. siamensis in the vicinity of Bangkok. Their ITS2 sequence for one of these (MW020049) had only partial coverage (72%) and low similarity (77%) with our mutabile sequence, suggesting that they are not conspecific.…”

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confidence: 92%

Molecular identification of trematode parasites infecting the freshwater snail Bithynia siamensis goniomphalos in Thailand

et al. 2022

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Digenetic trematodes are important parasites of humans and animals. They have complex life cycles and typically infect a gastropod as the first intermediate host. Bithynia siamensis goniomphalos, the first intermediate host of the liver fluke, Opisthorchis viverrini, harbours a wide variety of other trematode species. Morphological details of cercariae of 20 trematode taxa from B. s. goniomphalos, collected mainly in Thailand from 2009 to 2014, were provided in an earlier paper. Correct identification to the species or genus level based on morphology of these cercariae is generally not possible. Therefore, we used molecular data to improve identification and to investigate the diversity of the species of trematodes infecting B. s. goniomphalos. We were successful in extracting, amplifying and sequencing portions of the 28S ribosomal RNA (rRNA) gene for 19 of these 20 types of cercaria, and the internal transcribed spacer 2 region for 18 types. BLAST searches in GenBank and phylogenetic trees inferred from the 28S rRNA sequences identified members of at least nine superfamilies and 12 families. Only a few cercariae could be assigned confidently to genus or species on the basis of the sequence data. Matching sequence data from named adult trematodes will be required for definitive identification. There is clearly a great diversity of trematode species utilizing B. s. goniomphalos in Thailand.

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Exploring species diversity of lissorchiid trematodes (Digenea: Lissorchiidae) associated with the gravel snail,Lithoglyphus naticoides, in European freshwaters

Cited by 10 publications

References 39 publications

Phylogenetic Evidence for the Lissorchiid Concept of the Genus Anarhichotrema Shimazu, 1973 (Trematoda, Digenea)

Phylogenetic Evidence for the Lissorchiid Concept of the Genus Anarhichotrema Shimazu, 1973 (Trematoda, Digenea)

Diversity of European lissorchiid trematodes from fish and snail hosts with comments on the validity of the genusParasymphylodoraSzidat, 1943

Molecular identification of trematode parasites infecting the freshwater snail Bithynia siamensis goniomphalos in Thailand

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