This study examined the relationship among autistic traits, gender, and perceptions of artificial intelligence (AI) tools for second language (L2) speaking practice among neurotypical university students. The investigation included 111 participants and utilized the Broad Autism Phenotype Questionnaire to assess Autistic Traits (AU) and the sub-traits Aloof (AF), Rigid (RD), and Pragmatic Language (PL). It also employed various scales to measure perceptions, including Perceived Usefulness (PU), Perceived Ease of Use (PEOU), Attitude (AT), Behavioral Intention (BI), and Usage Behavior (UB). The findings indicated no significant differences in AU, AF, RD, and PL between males and females. Similarly, there were no significant differences in PU, AT, BI, and UB between genders, although males demonstrated marginally higher scores in PEOU than females. Autistic traits showed specific correlations with perceptions of AI tools: AU was negatively correlated with PU, AT, and marginally with PEOU; AF was marginally negatively correlated with PU and AT; RD was negatively correlated with PEOU, AT, and BI, and marginally with PU. Furthermore, the study highlighted the moderating role of gender in the relationship between autistic traits and perceptions of AI tools. Specifically, the effects of autistic traits on perceptions were more pronounced in males than in females, particularly the relationships between PEOU and AF, UB and AU, and UB and PL. This research highlighted the importance of considering autistic traits in educational technology deployment, advocating for customizable AI solutions to better meet diverse learner needs. The outcomes contributed to a more inclusive understanding of educational technology, emphasizing the need to adapt AI tools to enhance learning experiences for all students, regardless of gender or neurodiversity.