Exploring the anti-diabetic potential of Australian Aboriginal and Indian Ayurvedic plant extracts using cell-based assays

Gulati, Vandana; Gulati, Pankaj; Harding, Ian H.; Palombo, Enzo A.

doi:10.1186/s12906-015-0524-8

Cited by 32 publications

(20 citation statements)

References 51 publications

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“…However, only a limited number of species have been extensively studied. There has been limited previous research on Australian Aboriginal medicinal plants for activity against enzymes relevant to carbohydrate metabolism [21, 37] and to our knowledge, no previous studies on Australian medicinal plants for antiglycation activities and inhibition of ACE. Our research is a collaboration between Aboriginal and University-based researchers with research into the efficacy and safety of their traditional plant medicines driven and guided by Traditional Owners.…”

Section: Resultsmentioning

confidence: 99%

In vitro inhibitory activities of selected Australian medicinal plant extracts against protein glycation, angiotensin converting enzyme (ACE) and digestive enzymes linked to type II diabetes

Deo

Hewawasam

Karakoulakis

et al. 2016

BMC Complement Altern Med

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BackgroundThere is a need to develop potential new therapies for the management of diabetes and hypertension. Australian medicinal plants collected from the Kuuku I’yu (Northern Kaanju) homelands, Cape York Peninsula, Queensland, Australia were investigated to determine their therapeutic potential. Extracts were tested for inhibition of protein glycation and key enzymes relevant to the management of hyperglycaemia and hypertension. The inhibitory activities were further correlated with the antioxidant activities.MethodsExtracts of five selected plant species were investigated: Petalostigma pubescens, Petalostigma banksii, Memecylon pauciflorum, Millettia pinnata and Grewia mesomischa. Enzyme inhibitory activity of the plant extracts was assessed against α-amylase, α-glucosidase and angiotensin converting enzyme (ACE). Antiglycation activity was determined using glucose-induced protein glycation models and formation of protein-bound fluorescent advanced glycation endproducts (AGEs). Antioxidant activity was determined by measuring the scavenging effect of plant extracts against 1, 1-diphenyl-2-picryl hydrazyl (DPPH) and using the ferric reducing anti-oxidant potential assay (FRAP). Total phenolic and flavonoid contents were also determined.ResultsExtracts of the leaves of Petalostigma banksii and P. pubescens showed the strongest inhibition of α-amylase with IC50 values of 166.50 ± 5.50 μg/mL and 160.20 ± 27.92 μg/mL, respectively. The P. pubescens leaf extract was also the strongest inhibitor of α-glucosidase with an IC50 of 167.83 ± 23.82 μg/mL. Testing for the antiglycation potential of the extracts, measured as inhibition of formation of protein-bound fluorescent AGEs, showed that P. banksii root and fruit extracts had IC50 values of 34.49 ± 4.31 μg/mL and 47.72 ± 1.65 μg/mL, respectively, which were significantly lower (p < 0.05) than other extracts. The inhibitory effect on α-amylase, α-glucosidase and the antiglycation potential of the extracts did not correlate with the total phenolic, total flavonoid, FRAP or DPPH. For ACE inhibition, IC50 values ranged between 266.27 ± 6.91 to 695.17 ± 15.38 μg/mL.ConclusionsThe tested Australian medicinal plant extracts inhibit glucose-induced fluorescent AGEs, α-amylase, α-glucosidase and ACE with extracts of Petalostigma species showing the most promising activity. These medicinal plants could potentially be further developed as therapeutic agents in the treatment of hyperglycaemia and hypertension.Electronic supplementary materialThe online version of this article (doi:10.1186/s12906-016-1421-5) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

In vitro inhibitory activities of selected Australian medicinal plant extracts against protein glycation, angiotensin converting enzyme (ACE) and digestive enzymes linked to type II diabetes

Deo

Hewawasam

Karakoulakis

et al. 2016

BMC Complement Altern Med

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show abstract

“…Among the five fractions obtained, F5 gave the lowest IC 50 value. Even though the value was higher than the NCI reference value, plant extracts with IC 50 values within range of 30 to 200μg/ml have the potential to be refined into anticancer agents [19,22] Based on the overall evaluations of antiproliferative activities among PFME and its fractions, the IC 50 value of F5 was higher than the PFME. The findings suggested that the PFME was more potent than the fractions.…”

Section: Antiproliferative Studies Of Pfme and Fractions On Selected mentioning

confidence: 99%

Antiproliferative, Antioxidative and Compounds Identification from Methanolic Extract of Passiflora Foetida and Its Fractions

Sul’ain¹

2017

JAPLR

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“…On the other hand, preadipocytes were also maintained with fresh FBS-DMEM for the entire induction period. At the end of the induction period oil red O staining assay [35] was performed to monitor the degree of differentiation. For the comparison of triglyceride accumulation photo microscopic assessment was also carried out.…”

Section: Percent Viability = (Od Of Untreated Cells -Od Of Treated Cementioning

confidence: 99%

Development, Characterization and Antidiabetic Potentials of Nilgirianthusciliatus Nees Derived Nanoparticles

Kavitha¹,

Sujatha²,

Manoharan³

2017

J Nanomedine Biotherapeutic Discov

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Exploring the anti-diabetic potential of Australian Aboriginal and Indian Ayurvedic plant extracts using cell-based assays

Cited by 32 publications

References 51 publications

In vitro inhibitory activities of selected Australian medicinal plant extracts against protein glycation, angiotensin converting enzyme (ACE) and digestive enzymes linked to type II diabetes

In vitro inhibitory activities of selected Australian medicinal plant extracts against protein glycation, angiotensin converting enzyme (ACE) and digestive enzymes linked to type II diabetes

Antiproliferative, Antioxidative and Compounds Identification from Methanolic Extract of Passiflora Foetida and Its Fractions

Development, Characterization and Antidiabetic Potentials of Nilgirianthusciliatus Nees Derived Nanoparticles

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