Exploring the Antioxidant Potential of Phenolic Compounds from Winery By-Products by Hydroethanolic Extraction

Abstract

The residues generated in the wine industry (pomace, stems, seeds, wine lees, and grapevine shoots) are a potential source of bioactive compounds that can be used in other industries despite being sometimes underestimated. Different extraction methods using various solvents and extraction conditions are currently being investigated. Due to its natural occurrence in wines, safe behavior, and low toxicity when compared to other organic solvents, ethanol is used as an extracting agent. The aim of this study was to identify the winery by-product from the Região Demarcada do Douro and its corresponding extraction solvents that yields the most favorable results in (poly)phenols content and antioxidant capacity. To achieve this, five different ratios of ethanol: water, namely 0:100, 25:75, 50:50, 75:25, and 100:0 (v/v), for extracting the phenolic compounds were employed. Afterwards, the determination of total phenolic content (TPC), ortho-diphenols content (ODC), and flavonoid content (FC) as well as the antioxidant capacity of the obtained extracts using three different methods was performed. Since the best results of the spectrophotometric assays were obtained mostly with hydroethanolic extracts of stems (50:50, v/v), identification by HPLC-DAD has carried out. It was possible to conclude that the Tinta Roriz variety displayed the highest number of identified (poly)phenols.

In Vitro and In Silico Anti-Glioblastoma Activity of Hydroalcoholic Extracts of Artemisia annua L. and Artemisia vulgaris L.

“…Our results also have shown ( Table 1 ) that the yield of the phenolic compounds was not increased, and even had a tendency to be lower when β-cyclodextrin (5%) was used as an excipient during the hydroalcoholic extraction of polyphenols from A. annua and A. vulgaris . Other studies have shown that the efficiency of β-cyclodextrin in extracting a broad spectrum of phenolic compounds may not be equal to that of more traditional solvents [ 35 , 36 ]. Hydroalcoholic solutions, without the addition of cyclodextrins, can often extract a wider range of phenolics more efficiently due to their ability to dissolve both hydrophilic and hydrophobic compounds [ 35 , 36 ].…”

“…Other studies have shown that the efficiency of β-cyclodextrin in extracting a broad spectrum of phenolic compounds may not be equal to that of more traditional solvents [ 35 , 36 ]. Hydroalcoholic solutions, without the addition of cyclodextrins, can often extract a wider range of phenolics more efficiently due to their ability to dissolve both hydrophilic and hydrophobic compounds [ 35 , 36 ]. The use of titanium dioxide as an excipient in food and pharmaceutical products has been subject recently to regulatory scrutiny due to concerns over its safety, especially in nanoparticle form [ 28 ].…”

An Investigation on the Antioxidant Capabilities, Chemical Analysis and Potential Green Applications of the Yellow Bedstraw (Galium Verum) Ethanol Extract

“…Because antioxidant activity is by far the most important characteristic of plant extracts, the main determinations are focused on the identi cation and dosage of phenolic compounds (Abdelwahab et ( Sari et al, 2023) or statistical analysis (Costa et al, 2023).…”