Exploring the association between polymorphisms at 3’UTR SLC11A1 gene microsatellites and resistance to tuberculosis: A case-control study in Bos taurus dairy cattle

Hasenauer, Flavia C.; Garbaccio, Sergio; Caffaro, María Eugenia; Garro, Carlos; Huertas, Pablo; Poli, M. A.; Rossetti, C

doi:10.1016/j.livsci.2018.01.012

Cited by 3 publications

(4 citation statements)

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“…New strategies have been recommended, such as vaccination, in either cattle or the wildlife species [41], estimation of direct genomic estimated breeding values (EBVs) in UK dairy cattle [42], or to increase host resistance through breeding practices [43]. Recent studies in fact have disclosed genetic variability affecting resistance to bTB [20,41,43,44,45,46,47,48] suggesting the possibility of implementing genomic selection for that feature in cattle.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Association Study in Mexican Holstein Cattle Reveals Novel Quantitative Trait Loci Regions and Confirms Mapped Loci for Resistance to Bovine Tuberculosis

González-Ruiz

Strillacci

Durán-Aguilar

et al. 2019

Animals

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Simple SummaryBovine tuberculosis is an infectious disease of cattle caused by Mycobacterium bovis characterized by the formation of tubercles in any organ or tissue. Bovine tuberculosis represents a significant veterinary and public health problem in many parts of the world. It is zoonotic, transmitted to humans through consumption of infected milk and other cattle products. Although many factors influence infection and progression of the disease, there must be an important host genetic component that explains why some animals get sick and others remain healty. We present evidence of genetic variants associated with resistance to tuberculosis in Mexican Holstein dairy cattle using a case-control approach with a selective DNA pooling. Here, we identified novel quantitative trait loci regions harboring genes involved in Mycobacterium spp. immune response. This is a first screening about resistance to tuberculosis infection on Mexican Holstein cattle based on a dense single nucleotide polymorphism chip. The identified genes belong to both, the already known, and the undisclosed quantitative trait loci regions.AbstractBovine tuberculosis (bTB) is a disease of cattle that represents a risk to public health and causes severe economic losses to the livestock industry. Recently, genetic studies, like genome-wide association studies (GWAS) have greatly improved the investigation of complex diseases identifying thousands of disease-associated genomic variants. Here, we present evidence of genetic variants associated with resistance to TB in Mexican dairy cattle using a case-control approach with a selective DNA pooling experimental design. A total of 154 QTLRs (quantitative trait loci regions) at 10% PFP (proportion of false positives), 42 at 5% PFP and 5 at 1% PFP have been identified, which harbored 172 annotated genes. On BTA13, five new QTLRs were identified in the MACROD2 and KIF16B genes, supporting their involvement in resistance to bTB. Six QTLRs harbor seven annotated genes that have been previously reported as involved in immune response against Mycobacterium spp: BTA (Bos taurus autosome) 1 (CD80), BTA3 (CTSS), BTA 3 (FCGR1A), BTA 23 (HFE), BTA 25 (IL21R), and BTA 29 (ANO9 and SIGIRR). We identified novel QTLRs harboring genes involved in Mycobacterium spp. immune response. This is a first screening for resistance to TB infection on Mexican dairy cattle based on a dense SNP (Single Nucleotide Polymorphism) chip.

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Section: Discussionmentioning

confidence: 99%

Genome-Wide Association Study in Mexican Holstein Cattle Reveals Novel Quantitative Trait Loci Regions and Confirms Mapped Loci for Resistance to Bovine Tuberculosis

González-Ruiz

Strillacci

Durán-Aguilar

et al. 2019

Animals

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“…Conflicting data also exists regarding the role this microsatellite plays in resistance to bTB. Indeed, whereas a study performed in African zebu cattle found that higher numbers of GT repeats in the MS1 microsatellite were associated with a reduction in traits indicative of bTB ( Kadarmideen et al, 2011 ), a study using Argentine cattle found no association between different lengths of this microsatellite and response to the tuberculin skin test ( Hasenauer et al, 2018 ). If the MS1 microsatellite in the 3′UTR of the bovine SLC11A1 gene indeed plays a biological role in NRAMP1 expression, it is likely to be due to differences in post-transcriptional regulation of the mRNA, possibly by influencing mRNA stability, similarly as described for other molecules ( Citores et al, 2004 ; Chen et al, 2007 ).…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, no association between the MS1 microsatellite and response to the tuberculin skin test was found in a study performed using HF and Jersey cattle in Argentina ( Hasenauer et al, 2018 ). Furthermore, no associations between the MS2 microsatellite and susceptibility/resistance to bTB have currently been identified ( Barthel et al, 2000 ; Hasenauer et al, 2018 ). Therefore, in the present study we characterized polymorphisms in the bovine SLC11A1 gene in two B. taurus breeds, BS and HF, both present in the United Kingdom, and compared these to those found in Sahiwal cattle, a B. indicus breed.…”

Section: Introductionmentioning

confidence: 93%

“…Interestingly, an increase in the length of the MS1 microsatellite has been described to reduce traits indicative of bTB infection in African zebu cattle (Kadarmideen et al, 2011). In contrast, no association between the MS1 microsatellite and response to the tuberculin skin test was found in a study performed using HF and Jersey cattle in Argentina (Hasenauer et al, 2018). Furthermore, no associations between the MS2 microsatellite and susceptibility/resistance to bTB have currently been identified (Barthel et al, 2000;Hasenauer et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Analysis of Genetic Variation in the Bovine SLC11A1 Gene, Its Influence on the Expression of NRAMP1 and Potential Association With Resistance to Bovine Tuberculosis

et al. 2020

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Bovine tuberculosis (bTB), caused by Mycobacterium bovis , is a chronic zoonotic disease where host genetics is thought to contribute to susceptibility or resistance. One of the genes implicated is the SLC11A1 gene, that encodes for the natural resistance-associated macrophage protein 1 (NRAMP1). The aim of this study was to identify SLC11A1 polymorphisms and to investigate any resulting functional differences in NRAMP1 expression that might be correlated with resistance/susceptibility to M. bovis infection. Sequencing of the SLC11A1 gene in cDNA isolated from Brown Swiss, Holstein Friesian, and Sahiwal cattle identified five single nucleotide polymorphisms (SNPs) in the coding region, but only one of these (SNP4, c.1066C>G, rs109453173) was present in all three cattle breeds and therefore warranted further investigation. Additionally, variations of 10, 11, and 12 GT repeats were identified in a microsatellite (MS1) in the SLC11A1 3′UTR. Measurement of NRAMP1 expression in bovine macrophages by ELISA showed no differences between cells generated from the different breeds. Furthermore, variations in the length of the MS1 microsatellite did not impact on NRAMP1 protein expression as analyzed by luciferase reporter assay. However, further analysis of the ELISA data identified that the presence of the alternative G allele at SNP4 was associated with increased expression of NRAMP1 in bovine macrophages. Since NRAMP1 has been shown to influence the survival of intracellular pathogens such as M. bovis through the sequestering of iron, it is possible that cattle expressing the alternative G allele might have an increased resistance to bTB through increased NRAMP1 expression in their macrophages.

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Deletion in KARLN intron 5 and predictive relationship with bovine tuberculosis and brucellosis infection phenotype

Rossi

Caffaro²,

Raschia

et al. 2022

Vet Res Commun

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Exploring the association between polymorphisms at 3’UTR SLC11A1 gene microsatellites and resistance to tuberculosis: A case-control study in Bos taurus dairy cattle

Cited by 3 publications

References 32 publications

Genome-Wide Association Study in Mexican Holstein Cattle Reveals Novel Quantitative Trait Loci Regions and Confirms Mapped Loci for Resistance to Bovine Tuberculosis

Genome-Wide Association Study in Mexican Holstein Cattle Reveals Novel Quantitative Trait Loci Regions and Confirms Mapped Loci for Resistance to Bovine Tuberculosis

Analysis of Genetic Variation in the Bovine SLC11A1 Gene, Its Influence on the Expression of NRAMP1 and Potential Association With Resistance to Bovine Tuberculosis

Deletion in KARLN intron 5 and predictive relationship with bovine tuberculosis and brucellosis infection phenotype

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