Exploring the Biosynthesis of Natural Products and Their Inherent Suitability for the Rational Design of Desirable Compounds through Genetic Engineering

Isocyanide Heterodimerization-Triggered Three-Component Reaction: Diversity-Oriented Synthesis of Quinoxalines

“…The quinoxaline core represents a privileged substructure in numerous biologically active natural products, pharmaceuticals, and agrochemicals . Consequently, the development of green and sustainable methodologies for the synthesis of quinoxalines is still highly attractive .…”

Labelling studies in the biosynthesis of polyketides and non-ribosomal peptides

“…11,12 Both structure elucidation and access to advanced isotopically labelled precursors often benefit from or even require the achievements of modern organic synthesis approaches. Recent advances have been made possible by the developments of genetic engineering, 13–15 structural biology, 16,17 computer technology, and analytical chemistry. These techniques enable the study of biosynthetic pathways through gene knockout and heterologous gene expression, in vitro reactions with recombinant enzymes, bioinformatics 18,19 and computational chemistry, 20 while a deep understanding of enzyme mechanisms can be achieved through structure-based site-directed mutagenesis.…”

Merging Whole‐cell Biosynthesis of Styrene and Transition‐metal Catalyzed Derivatization Reactions

“…The ever increasing understanding of biosynthetic pathways and the associated enzymes, enable the creation of new biosynthetic routes to obtain non-natural molecules via metabolic engineering of microorganisms. [1][2][3][4][5] Whereas biosynthesis is highly versatile and can be used to synthesize a wide range of interesting molecules, it uses a relatively limited number of reaction classes to do so, at least in comparison to organic synthesis. Hence, combining biosynthesis with transition-metal catalysis may dramatically expand the synthetic scope of biosynthesis.…”