Basic wood density (BWD) is a functional characteristic important to quantify carbon storage and is related to tree growth and its survival. We investigated BWD variability among tree species, populations, and forest types across the Atlantic Forest biome in Rio de Janeiro state, Brazil. We sampled and measured 576 trees of 83 species in 28 natural ecosystems in the Dense Ombrophilous Forest, Seasonal Semi-deciduous Forest, and the Restinga Forest. Stem wood from two different heights at each tree was acquired using a drill borer; discs were removed from branches in 20% of the height from 39 trees. BWD means increased from 0.42 for the most humid ecosystem to 0.675 g⋅cm−3 from the driest ecosystem. We identified changes in the wood as a result of climatic variables particular to different typologies, such as increased wood density in areas with lower precipitation and higher temperatures, were also observed within species, but with little difference in means. We identified three groups of species according to their BWD, different in mean height and diameter. The branches can be used to estimate tree wood density.