Exploring the genetic and functional diversity of <i>Porphyromonas gingivalis</i> long fimbriae

Meyer, Hendrik Leonhard; Abdelbary, Mohamed M. H.; Buhl, Eva Miriam; Kuppe, Christoph; Conrads, Georg

doi:10.1111/omi.12433

Cited by 3 publications

(1 citation statement)

References 69 publications

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“…The most studied, more virulent A7436 and W83 strains, belong to encapsulated strains, whereas the less virulent ATCC 33277 strain does not produce a capsule ( 22 , 28 , 33 , 36 – 39 , 82 , 83 ). P. gingivalis produces major and minor fimbriae, the main components of which, the FimA and Mfa1 proteins, respectively, differ significantly in their structures ( 24 , 30 , 31 , 39 , 83 – 87 ). Moreover, different types of fimbriae are produced by P. gingivalis strains, which is often correlated with pathogenicity ( 87 , 88 ).…”

Section: Discussionmentioning

confidence: 99%

Comparative analysis of Porphyromonas gingivalis A7436 and ATCC 33277 strains reveals differences in the expression of heme acquisition systems

Śmiga,

Ślęzak,

Olczak

2024

Microbiol Spectr

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Porphyromonas gingivalis strains exhibit different phenotypes in vitro , different virulence potential in animal models, and different associations with human diseases, with strains classified as virulent/more virulent (e.g., A7436 and W83) or as less virulent/avirulent (e.g., ATCC 33277). In this study, we comparatively analyzed the A7436 and ATCC 33277 strains to better understand their variability. Global gene expression analysis in response to heme and iron limitation revealed more pronounced differences in the A7436 than in the ATCC 33277 strain; however, in both strains, the largest changes were observed in genes encoding hypothetical proteins, genes whose products participate in energy metabolism, and in genes encoding proteins engaged in transport and binding proteins. Our results confirmed that variability between P. gingivalis strains is due to differences in the arrangement of their genomes. Analysis of gene expression of heme acquisition systems demonstrated that not only the availability of iron and heme in the external environment but also the ability to store iron intracellularly can influence the P. gingivalis phenotype. Therefore, we assume that differences in virulence potential may also be due to differences in the production of systems involved in iron and heme acquisition, mainly the Hmu system. In addition, our study showed that hemoglobin, in a concentration-dependent manner, differentially influences the virulence potential of P. gingivalis strains. We conclude that iron and heme homeostasis may add to the variability observed between P. gingivalis strains. IMPORTANCE Periodontitis belongs to a group of multifactorial diseases, characterized by inflammation and destruction of tooth-supporting tissues. P. gingivalis is one of the most important microbial factors involved in the initiation and progression of periodontitis. To survive in the host, the bacterium must acquire heme as a source of iron and protoporphyrin IX. P. gingivalis strains respond differently to changing iron and heme concentrations, which may be due to differences in the expression of systems involved in iron and heme acquisition. The ability to accumulate iron intracellularly, being different in more and less virulent P. gingivalis strains, may influence their phenotypes, production of virulence factors (including proteins engaged in heme acquisition), and virulence potential of this bacterium.

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Section: Discussionmentioning

confidence: 99%

Comparative analysis of Porphyromonas gingivalis A7436 and ATCC 33277 strains reveals differences in the expression of heme acquisition systems

Śmiga,

Ślęzak,

Olczak

2024

Microbiol Spectr

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Molecular Study of Porphyromonas Gingivalis Strains With fimA Genotypes in Periodontitis Patients

F. Faruq,

Khalaf Jabar

2023

Iran J Med Microbiol

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Frequency of Fimbrial Gene Types I, Ib, and II in Clinical Strains of Porphyromonas gingivalis Characterized From Periodontitis Patients

R.,

Girija,

Priyadharsini

et al. 2024

Cureus

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Objective Porphyromonas gingivalis ( P. gingivalis ) is considered the predominant pathogen in association with different stages of periodontitis, and fim genes play a vital role in adherence and colonization. This study is thus aimed to detect the prevalence of P. gingivalis and the frequency of fim gene types among the clinical strains isolated from periodontitis patients. Methods Plaque samples (N = 45) were collected from patients with three different stages of periodontitis (n = 15 in each group). All the samples were inoculated onto sterile anaerobic blood agar and were processed anaerobically using a GasPak system at 37°C for five to seven days. Standard microbiological techniques were used to identify P. gingivalis . Genomic DNA was extracted, and polymerase chain reaction (PCR) was carried out to detect the frequency of three fim gene types, using specific primers. Results P. gingivalis was more prevalent in Group III (93.3%), followed by 26.7% in Group II, and 13.3% in Group I. Maximum isolates were seen in the age group of 40-50, with no significance within the genders. fim type I was frequent in Group III (78.5% (n = 11)), followed by 0.25% (n = 1) under Group II, with no other fim types in the other groups. Conclusion Prevalence of P. gingivalis and frequency of fim genes, in association with its virulence, were observed. Periodical monitoring of such virulence genes would aid in the theranostic approach to combat the complications caused by P. gingivalis in periodontitis cases.

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Exploring the genetic and functional diversity of Porphyromonas gingivalis long fimbriae

Cited by 3 publications

References 69 publications

Comparative analysis of Porphyromonas gingivalis A7436 and ATCC 33277 strains reveals differences in the expression of heme acquisition systems

Comparative analysis of Porphyromonas gingivalis A7436 and ATCC 33277 strains reveals differences in the expression of heme acquisition systems

Molecular Study of Porphyromonas Gingivalis Strains With fimA Genotypes in Periodontitis Patients

Frequency of Fimbrial Gene Types I, Ib, and II in Clinical Strains of Porphyromonas gingivalis Characterized From Periodontitis Patients

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