Exploring the genome of Arctic Psychrobacter sp. DAB_AL32B and construction of novel Psychrobacter-specific cloning vectors of an increased carrying capacity

Ciok, Anna; Dziewit, Łukasz

doi:10.1007/s00203-018-1595-y

Cited by 6 publications

(13 citation statements)

References 63 publications

(75 reference statements)

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“…The available draft genomic data for Psychrobacter sp. DAB_AL32B [22] was insufficient to permit the assembly of the complete nucleotide sequence of plasmid pP32BP2. Therefore, plasmid DNA was isolated and sequenced de novo using Illumina technology.…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, plasmid DNA was isolated and sequenced de novo using Illumina technology. An analysis of the assembled sequence revealed that the size of pP32BP2 is 54,438 bp, with an average GC content of 39.9%, which is lower than that of the DAB_AL32B chromosome (41.9%) [22], but similar to the average value for other Psychrobacter plasmids (39.2%) (GenBank). The manual annotation of the pP32BP2 sequence identified 54 genes, which constitute 77.9% of the plasmid genome.…”

Section: Resultsmentioning

confidence: 99%

“…As Psychrobacter spp. serve as model cold-active and biotechnologically-valuable microorganisms, some of their plasmids have been used for the construction of cloning vectors to enable genetic engineering of psychrophilic bacteria [22,23,24].…”

Section: Introductionmentioning

confidence: 99%

“…Previously, we described the general genomic content and chromosomally-encoded adaptive features of the psychrophilic plasmid-containing Arctic bacterium Psychrobacter sp. DAB_AL32B [8,22]. This strain was isolated from the feces of little auks (dovekie; Alle alle ) collected on Spitsbergen island (a breeding colony situated on a mountain slope in Ariekammen Isbjornhamna Bay—Hornsund Fjord; 77°00′37.0″N, 15°31′49.5″E) [8].…”

Section: Introductionmentioning

confidence: 99%

“…The DAB_AL32B strain carries two circular plasmids: a small cryptic replicon pP32BP1 (4,599 bp) and a larger plasmid pP32BP2 (54,438 bp) [8,22]. In this study, we performed a detailed structural and functional analysis of pP32BP2, which revealed the antagonistic influence of this plasmid on the metabolic and physiological properties of its host strain.…”

Section: Introductionmentioning

confidence: 99%

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Benefits and Drawbacks of Harboring Plasmid pP32BP2, Identified in Arctic Psychrophilic Bacterium Psychrobacter sp. DAB_AL32B

Ciok

Cegielski

Bartosik

et al. 2019

IJMS

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Psychrobacter sp. DAB_AL32B, originating from Spitsbergen island (Arctic), carries the large plasmid pP32BP2 (54,438 bp). Analysis of the pP32BP2 nucleotide sequence revealed the presence of three predicted phenotypic modules that comprise nearly 30% of the plasmid genome. These modules appear to be involved in fimbriae synthesis via the chaperone-usher pathway (FIM module) and the aerobic and anaerobic metabolism of carnitine (CAR and CAI modules, respectively). The FIM module was found to be functional in diverse hosts since it facilitated the attachment of bacterial cells to abiotic surfaces, enhancing biofilm formation. The CAI module did not show measurable activity in any of the tested strains. Interestingly, the CAR module enabled the enzymatic breakdown of carnitine, but this led to the formation of the toxic by-product trimethylamine, which inhibited bacterial growth. Thus, on the one hand, pP32BP2 can enhance biofilm formation, a highly advantageous feature in cold environments, while on the other, it may prevent bacterial growth under certain environmental conditions. The detrimental effect of harboring pP32BP2 (and its CAR module) seems to be conditional, since this replicon may also confer the ability to use carnitine as an alternative carbon source, although a pathway to utilize trimethylamine is most probably necessary to make this beneficial. Therefore, the phenotype determined by this CAR-containing plasmid depends on the metabolic background of the host strain.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Benefits and Drawbacks of Harboring Plasmid pP32BP2, Identified in Arctic Psychrophilic Bacterium Psychrobacter sp. DAB_AL32B

Ciok

Cegielski

Bartosik

et al. 2019

IJMS

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Structure and functions of a multireplicon genome of Antarctic Psychrobacter sp. ANT_H3: characterization of the genetic modules suitable for the construction of the plasmid-vectors for cold-active bacteria

et al. 2023

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Among Psychrobacter spp., there are several multireplicon strains, carrying more than two plasmids. Psychrobacter sp. ANT_H3 carries as many as 11 extrachromosomal replicons, which is the highest number in Psychrobacter spp. Plasmids of this strain were subjected to detailed genomic analysis, which enables an insight into the structure and functioning of this multireplicon genome. The replication and conjugal transfer modules of ANT_H3 plasmids were analyzed functionally to discover their potential for being used as building blocks for the construction of novel plasmid-vectors for cold-active bacteria. It was shown that two plasmids have a narrow host range as they were not able to replicate in species other than Psychrobacter, while remaining plasmids had a wider host range and were functional in various Alpha- and Gammaproteobacteria. Moreover, it was confirmed that mobilization modules of seven plasmids were functional, i.e., could be mobilized for conjugal transfer by the RK2 conjugation system. Auxiliary genes were also distinguished in ANT_H3 plasmids, including these encoding putative DNA-protecting protein DprA, multidrug efflux SMR transporter of EmrE family, glycine cleavage system T protein, MscS small-conductance mechanosensitive channel protein, and two type II restriction-modification systems. Finally, all genome-retrieved plasmids of Psychrobacter spp. were subjected to complex genome- and proteome-based comparative analyses showing that Antarctic replicons are significantly different from plasmids from other locations.

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Heterologous protein production using Psychrobacter sp. PAMC 21119 analyzed with a green fluorescent protein-based reporter system

Lee

Kim

et al. 2023

Protein Expression and Purification

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Exploring the genome of Arctic Psychrobacter sp. DAB_AL32B and construction of novel Psychrobacter-specific cloning vectors of an increased carrying capacity

Cited by 6 publications

References 63 publications

Benefits and Drawbacks of Harboring Plasmid pP32BP2, Identified in Arctic Psychrophilic Bacterium Psychrobacter sp. DAB_AL32B

Benefits and Drawbacks of Harboring Plasmid pP32BP2, Identified in Arctic Psychrophilic Bacterium Psychrobacter sp. DAB_AL32B

Structure and functions of a multireplicon genome of Antarctic Psychrobacter sp. ANT_H3: characterization of the genetic modules suitable for the construction of the plasmid-vectors for cold-active bacteria

Heterologous protein production using Psychrobacter sp. PAMC 21119 analyzed with a green fluorescent protein-based reporter system

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