Exploring the identity of the Greek Dinophysis cf. acuminata

Papaefthimiou, Dimitra; Aligizaki, Katerina; Nikolaidis, Georgios

doi:10.1016/j.hal.2010.05.005

Cited by 16 publications

(17 citation statements)

References 67 publications

(98 reference statements)

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“…Tree topologies generated using ML and BI were identical, and the phylogenetic relationships among the species were similar to those reported previously (Papaefthimiou et al. , Raho et al. ).…”

Section: Resultssupporting

confidence: 81%

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Characterization and comparison of toxin‐producing isolates of Dinophysis acuminata from New England and Canada

Tong

Smith

Richlen

et al. 2014

Journal of Phycology

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Following the identification of the first toxic isolate of Dinophysis acuminata from the northwestern Atlantic, we conducted detailed investigations into the morphology, phylogeny, physiology, and toxigenicity of three isolates from three sites within the northeastern U.S./Canada region: Eel Pond and Martha’s Vineyard, Massachusetts, and the Bay of Fundy. Another isolate, collected from the Gulf of Mexico, was grown under the same light, temperature, and prey conditions for comparison. Despite observed phenotypic heterogeneity, morphometrics and molecular evidence classified the three northwestern Atlantic isolates as Dinophysis acuminata Claparède & Lachmann, whereas the isolate from the Gulf of Mexico was morphologically identified as D. cf. ovum. Physiological and toxin analyses supported these classifications, with the three northwestern Atlantic isolates being more similar to each other with respect to growth rate, toxin profile, and diarrhetic shellfish poisoning (DSP) toxin content (okadaic acid + dinophysistoxin 1/cell) than they were to the isolate from the Gulf of Mexico, which had toxin profiles similar to those published for D. cf. ovum F. Schütt. The DSP toxin content, 0.01 – 1.8 pg okadaic acid (OA) + dinophysistoxin (DTX1) · cell−1, of the three northwestern Atlantic isolates was low relative to other D. acuminata strains from elsewhere in the world, consistent with the relative scarcity of shellfish harvesting closures due to DSP toxins in the northeastern U.S. and Canada. If this pattern is repeated with analyses of more geographically and temporally dispersed isolates from the region, it would appear that the risk of significant DSP toxin outbreaks in the northwestern Atlantic is low to moderate. Finally, the morphological, physiological, and toxicological variability within D. acuminata may reflect spatial (and/or temporal) population structure, and suggests that sub-specific resolution may be helpful in characterizing bloom dynamics and predicting toxicity.

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Section: Resultssupporting

confidence: 81%

“…Phylogenetic analysis. Tree topologies generated using ML and BI were identical, and the phylogenetic relationships among the species were similar to those reported previously (Papaefthimiou et al 2010, Raho et al 2013). These analyses recovered three well-supported main clades (Fig.…”

Section: Resultssupporting

confidence: 78%

Characterization and comparison of toxin‐producing isolates of Dinophysis acuminata from New England and Canada

Tong

Smith

Richlen

et al. 2014

Journal of Phycology

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“…acuminata is the dominant Dinophysis species in these areas (Koukaras & Nikolaidis 2004, Nikolaidis et al 2005.This a species has a preference for seawater temperatures common during the winter and spring months (Table 4) in Greece (Ninčević-Gladan et al 2008). In a recent study, Papaefthimiou et al (2010), using ribosomal and mitochondrial genetic sequence data, re-identified the D. cf. acuminata described from Greek coastal waters as D. cf.…”

Section: Air Temperaturementioning

confidence: 99%

Climate influence on Dinophysis spp. spatial and temporal distributions in Greek coastal water

Vlamis¹,

Katikou²

2014

Plankton Benthos Res

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Presence of Dinophysis spp., the main phytoplankton genus responsible for the occurrence of lipophilic biotoxins of the okadaic acid group (Diarrhetic Shellfish Poisoning; DSP) in Greek mussels, was recorded in the period 2003-2008 in five major mussel culture areas of Greece. Dinophysis spp. abundances were simultaneously studied with climatological parameters (air temperature, rainfall, wind direction and velocity) and the occurrence of DSP toxic episodes in mussels. Dinophysis spp. blooms occurred with a seasonal periodicity. Highest cell abundances of the bloom appeared steadily between late winter and spring, whereas occurrence of DSP toxicity in mussels followed a similar time pattern, with mouse bioassay-positive samples ranging between 10.2-28.1% of the total samples analysed. Maximum mean Dinophysis spp. counts coincided with air temperatures in the range of 10-20°C; abundances higher than 1,000 cells L 1 were more frequently recorded at air temperatures in the range of 4-20°C in combination with low rainfall and low velocity winds (

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“…Plastid genes such as psbA and plastid SSU rDNA could be considered as alternative markers, but it is unlikely that the genes will provide adequate tools for species delineation in Dinophysis species because these dinoflagellates often have polymorphic plastid signatures in the same cell (see below). More recently, mitochondrial DNA markers such as cob and cox1 have been successfully used in phylogenetic studies (Zhang et al, 2008); Raho et al (2008) demonstrated that sequences of the cox1 gene were variable by as much as 25 bp between D. acuminata and D. ovum from Galician waters and Papaefthimiou et al (2010), again using the cox1 gene, found that D. cf acuminata described from Thermaikos Bay (Greece) is actually more related to D. ovum. Nonetheless, there are at present only a limited number of cox1 sequences available from Dinophysis species.…”

Section: Morphology and Phylogeneticsmentioning

confidence: 99%