Exploring the lignolytic potential of a new laccase producing strain Kocuria sp. PBS-1 and its application in bamboo pulp bleaching

Boruah, Paran; Sarmah, Pallab; Das, P.; Goswami, Tridip

doi:10.1016/j.ibiod.2019.104726

Cited by 29 publications

(2 citation statements)

References 83 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To further characterize the role of GNAI in P. eryngii , the key enzyme activities were measured in the mycelial growth phase of transformed strains ( Figure 2 ). Changes in reducing sugar content, amylase activity, and laccase activity could reflect the ability of hyphae to degrade macromolecules and utilize nutrients during growth [ 28 , 36 ]. Hu et al revealed the significant attenuation of carbon metabolism including the metabolic pathways of starch and sucrose by knocking down the putative Gα-encoding gene gna1 of coccidia to block the heterologous Gα-cAMP/PKA pathway [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis Reveals the Function of a G-Protein α Subunit Gene in the Growth and Development of Pleurotus eryngii

Cao

Sun

et al. 2023

JoF

View full text Add to dashboard Cite

Pleurotus eryngii is a commercially important edible fungus with high nutritional and economic value. However, few functional studies have examined key genes affecting the growth and development of P. eryngii. In this study, transformed strains, including over-expression (PeGNAI-OE) and RNA interference (PeGNAI-RNAi) lines, were constructed to elucidate the role of GNAI in P. eryngii growth. GNAI expression was found to affect the mycelial growth and the number of clamp connections. Moreover, the transformed strains were shown to have higher endogenous cAMP levels, thus affecting amylase and laccase activity. Fruiting experiments showed that GNAI expression revealed the formation of P. eryngii primordia and the number of buttons, while transcription analysis identified GNAI gene involvement in the growth and development of P. eryngii. Seven downstream genes regulated by GNAI were differentially expressed in PeGNAI-OE and PeGNAI-RNAi compared to wild type (WT). These genes may be related to mycelial growth and enzyme activity. They were involved in the MAPK signaling pathway, inositol phosphate metabolism, ascorbate, aldarate metabolism, and starch and sucrose metabolism. In summary, GNAI performs different physiological functions in regulating the growth and development of P. eryngii. Importantly, the molecular mechanisms of GNAI regulatory function are relatively complex and need further study.

show abstract

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis Reveals the Function of a G-Protein α Subunit Gene in the Growth and Development of Pleurotus eryngii

Cao

Sun

et al. 2023

JoF

View full text Add to dashboard Cite

show abstract

“…White-rot fungi (species or strains of Basidiomycota) have the ability to degrade lignocellulosic wastes with high efficiency because they could secret various extracellular enzymes (Kuhar et al 2015;Rodrigues et al 2019). Recently, there has been increased research attention on screening fungal or bacterial strains suitable for obtaining massive high-activity laccase in solid-state fermentation (Al-balawi et al 2017;Sun et al 2017;Boruah et al 2019;Wang et al 2019;Yasar et al 2019;Dullah et al 2021;Sun et al 2021b). In recent years, novel fungi with the ability to produce ligninolytic enzymes have been isolated from different natural habitats.…”

Section: Effect Of Fungi In Same or Different Genus On Laccase Activitymentioning

confidence: 99%

Extracellular laccase activity among Ganoderma and Coriolopsis species grown on lignocellulosic wastes

Han

Yang

Jian-jun

et al. 2022

BioRes

View full text Add to dashboard Cite

The extensive application of laccase in various fields depends on the supply of a large amount of laccase with high activity and low cost. Interest on the screening of fungal strains suitable for obtaining massive high-activity laccase in solid-state fermentation with suitable low-cost lignocellulosic wastes has increased. The present work determined the laccase secretion from different species belonging to genera Ganoderma and Coriolopsis fermented on different lignocellulosic wastes. Maximal laccase activity was obtained from Ganoderma lingzhi Han 1345 grown on rice straw for 9 days and indicated the capacity of secreting laccase of G. lingzhi Han 1345 was superior to that of Coriolopsis trogii Han 1211, C. strumosa Han 1356, and G. applanatum Han 1578. The presence of cottonseed hull was more favorable for Coriolopsis species secreting laccase, while Ganoderma species were more preferred to secrete laccase on rice straw. Further, the consistent substrate preference in laccase production of different species in the same genus was first exhibited. The results are useful to screen and obtain new species with superior capacity of secreting laccase and suitable low-cost lignocellulosic wastes as fermentation substrate for further industrial application of laccase.

show abstract

Ligninase in Degradation of Lignocellulosic Wastes

Gunjal¹,

Patil²,

Shinde

2020

Enzymes in Degradation of the Lignocellulosic Wastes

View full text Add to dashboard Cite

Exploring the lignolytic potential of a new laccase producing strain Kocuria sp. PBS-1 and its application in bamboo pulp bleaching

Cited by 29 publications

References 83 publications

Transcriptome Analysis Reveals the Function of a G-Protein α Subunit Gene in the Growth and Development of Pleurotus eryngii

Transcriptome Analysis Reveals the Function of a G-Protein α Subunit Gene in the Growth and Development of Pleurotus eryngii

Extracellular laccase activity among Ganoderma and Coriolopsis species grown on lignocellulosic wastes

Ligninase in Degradation of Lignocellulosic Wastes

Contact Info

Product

Resources

About