Exploring the potential of novel R. kratochvilovae red yeasts towards the sustainable synthesis of natural carotenoids

Sereti, Fani; Papadaki, Aikaterini; Alexandri, Maria; Kachrimanidou, Vasiliki; Kopsahelis, Nikolaos

doi:10.1016/j.scp.2022.100927

Cited by 6 publications

(8 citation statements)

References 46 publications

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“…Lopes et al [28] reported the change from C/N = 80 to C/N = 120 of β-carotene, torularhodin, and torulene produced by R. toruloides. R. kratochvilovae Y-42 produced up to 2.59 mg/L of carotenoids in a glucose-based medium at C/N = 80 [29]. The production capacity of torularhodin (63%, 50.5 3.0 g/g) from R. mucilaginosa was evaluated in a media containing xylose and glycerol.…”

Section: Medium Compositionmentioning

confidence: 99%

Current Advances in Carotenoid Production by Rhodotorula sp.

Ochoa-Viñals,

Alonso-Estrada,

Pacios-Michelena

et al. 2024

Fermentation

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Microbial carotenoids are pigments of lipophilic nature; they are considered promising substitutes for chemically synthesized carotenoids in the food industry. Their benefits for human health have been demonstrated due to their antioxidant capacity. Yeasts of the genus Rhodotorula have genotypic characteristics that allow them to accumulate high concentrations of carotenes under certain stress conditions. The present review includes recent information covering different aspects of carotenoid production in Rhodotorula sp. fermentation. This review focuses on fermentation carotenoid production strategies, describing various economic raw materials as sources of carbon and nitrogen, the capacity for tolerance to heavy metals, and the effect of light, pH, and salts on the accumulation of carotenoids. Genetic modification strategies used to obtain strains with increased carotenoid production are described. Furthermore, using magnetic nanoparticles in the fermentation system, which could be a stress factor that increases pigment production, is considered for the first time. Rhodotorula is a potential source of high-value carotenoids with applications in the cosmetics, pharmaceutical, and food industries.

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Section: Medium Compositionmentioning

confidence: 99%

Current Advances in Carotenoid Production by Rhodotorula sp.

Ochoa-Viñals,

Alonso-Estrada,

Pacios-Michelena

et al. 2024

Fermentation

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“…The efficient productivities and high yields, complete process control and standardization of the product quality confer advantages of microbial carotenoids' synthesis, directly related to the potential for large-scale production. Moreover, the utilization of low-cost and renewable resources (such as agro-industrial wastes and byproducts) as substrates for microbial growth could further mitigate the overall cost of production [28].…”

Section: Microorganismsmentioning

confidence: 99%

“…Chlorella and Scenedesmus are also significant carotenoid producers, mainly of lutein [32,33]. The limitation of large-scale carotenoid production using microalgae lies in the high production costs and the high land requirements [28,34]. Bacteria and fungi introduce a prevalent advantage to this angle, as proper strain selection, substrate and fermentation/bioreactor design can lead to high product yields and productivities in completely controlled processes.…”

Section: Biotechnological Production Of Carotenoidsmentioning

confidence: 99%

“…together with the genera Rhodosporidium, Sporidiobolus and Sporobolomyces belong to a category known as "red yeasts", describing their ability to intracellularly accumulate carotenoids [35]. Another important attribute of yeasts is their capability to synthesize carotenoids via the valorization of low-cost substrates as growth substrates, including cheese whey [28], molasses [36] and raw glycerol [37]. Red yeasts have been generally reported to produce mixtures of carotenoids, mainly β-carotene, γ-carotene, lycopene, torulene and torularhodin [28,35].…”

Section: Biotechnological Production Of Carotenoidsmentioning

confidence: 99%

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Natural Carotenoids: Recent Advances on Separation from Microbial Biomass and Methods of Analysis

et al. 2023

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Biotechnologically produced carotenoids occupy an important place in the scientific research. Owing to their role as natural pigments and their high antioxidant properties, microbial carotenoids have been proposed as alternatives to their synthetic counterparts. To this end, many studies are focusing on their efficient and sustainable production from renewable substrates. Besides the development of an efficient upstream process, their separation and purification as well as their analysis from the microbial biomass confers another important aspect. Currently, the use of organic solvents constitutes the main extraction process; however, environmental concerns along with potential toxicity towards human health necessitate the employment of “greener” techniques. Hence, many research groups are focusing on applying emerging technologies such as ultrasounds, microwaves, ionic liquids or eutectic solvents for the separation of carotenoids from microbial cells. This review aims to summarize the progress on both the biotechnological production of carotenoids and the methods for their effective extraction. In the framework of circular economy and sustainability, the focus is given on green recovery methods targeting high-value applications such as novel functional foods and pharmaceuticals. Finally, methods for carotenoids identification and quantification are also discussed in order to create a roadmap for successful carotenoids analysis.

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“…They have the ability to grow quickly in several substrates, thereby significantly reducing the production time that would be required at an industrial scale [20]. Currently, the fermentation of microbial carotenoids is being extensively researched using different red yeasts, including Phaffia rhodozyma [21], Sporidiobolus pararoseus [11], Rhodosporidium kratochvilovae [22], Rhodosporidium toruloides [8], Rhodotorula sp. [23], Rhodotorula glutinis [24,25], Rhodotorula mucilaginosa [26,27], and Rhodotorula paludigina CM33 [4].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Lipid and Carotenoid Production via Rhodotorula paludigena CM33 Using Crude Glycerol as the Main Substrate: Pilot-Scale Experiments

Sriphuttha,

Boontawan,

Boonyanan

et al. 2023

IJMS

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Rhodotorula paludigena CM33 is an oleaginous yeast that has been demonstrated to accumulate substantial quantities of intracellular lipids and carotenoids. In this study, crude glycerol, a by-product of biodiesel production, was used as a carbon source to enhance the accumulation of lipids and carotenoids in the cells. The culture conditions were first optimized using response surface methodology, which revealed that the carotenoid concentration and lipid content improved when the concentration of crude glycerol was 40 g/L. Different fermentation conditions were also investigated: batch, repeated-batch, and fed-batch conditions in a 500 L fermenter. For fed-batch fermentation, the maximum concentrations of biomass, lipids, and carotenoids obtained were 46.32 g/L, 37.65%, and 713.80 mg/L, respectively. A chemical-free carotenoid extraction method was also optimized using high-pressure homogenization and a microfluidizer device. The carotenoids were found to be mostly beta-carotene, which was confirmed by HPLC (high pressure liquid chromatography), LC-MS (liquid chromatography-mass spectrometry), and NMR (nuclear magnetic resonance). The results of this study indicate that crude glycerol can be used as a substrate to produce carotenoids, resulting in enhanced value of this biodiesel by-product.

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Exploring the potential of novel R. kratochvilovae red yeasts towards the sustainable synthesis of natural carotenoids

Cited by 6 publications

References 46 publications

Current Advances in Carotenoid Production by Rhodotorula sp.

Current Advances in Carotenoid Production by Rhodotorula sp.

Natural Carotenoids: Recent Advances on Separation from Microbial Biomass and Methods of Analysis

Simultaneous Lipid and Carotenoid Production via Rhodotorula paludigena CM33 Using Crude Glycerol as the Main Substrate: Pilot-Scale Experiments

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