Exploring the potential of photosynthetic induction factor for the commercial production of fucoxanthin in Phaeodactylum tricornutum

Shenrui, LI; Zheng, Xiaodong; Fang, Qingshu; Yifu, Gong; Wang, Heyu

doi:10.1007/s00449-021-02559-x

Cited by 3 publications

(2 citation statements)

References 31 publications

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“…157,158 Although many species of microalgae are capable of producing fucoxanthin, only a small number of them have been explored for producing the compound commercially. 158,159 Among these are Chaetoceros calcitrans, 160 Chaetoceros gracilis, 161 Cylindrotheca Closterium, 162 Halamphora coffeaeformis, 163 Isochrysis galbana, 164 Phaeodactylum tricornutum 165,166 and Odontella aurita. 159 A Korean group has found that high levels of fucoxanthin could be accumulated in the microalga Isochrysis.…”

Section: Cultivation Of Microalgae To Produce Fucoxanthinmentioning

confidence: 99%

Mixotrophic cultivation of microalgae for carotenoid production

Liaqat

Khazi

Bahadar

et al. 2022

Reviews in Aquaculture

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The intrinsic ability of microalgae to accumulate high amounts of carotenoids has made them the preferred aquatic organisms of biotechnological exploration for carotenoid production. To continuously innovate and modify microalgal bioprocesses, aquaculture scientists have been working hard for the past decades in a forwardlooking way, and mixotrophic cultivation of microalgae is deemed as a promising strategy to decrease production cost. This review is intended to summarise the recent research advancement of carotenoids production from mixotrophically cultivated microalgae, starting from the structure, biosynthesis, physiological roles and applications of carotenoids and followed by the production processes both currently established and under development. Most importantly, the microalgal physiology of mixotrophic cultivation is reviewed in depth both in general and specifically for the most studied species, and the prospects of commercially viable mixotrophic microalgal processes for carotenoid production along with the insight of future research are of course discussed. Finally, we conclude that mixotrophy might be a promising strategy for large-scale cultivation of microalgae to produce carotenoids although some technical obstacles need to be overcome.

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Section: Cultivation Of Microalgae To Produce Fucoxanthinmentioning

confidence: 99%

Mixotrophic cultivation of microalgae for carotenoid production

Liaqat

Khazi

Bahadar

et al. 2022

Reviews in Aquaculture

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“…In contrast, the two-stage culture mode separates periods of microalgal growth and product accumulation. In Stage I, provided sufficient cell factories are provided, while and Stage II optimizes growth conditions (e.g, autotrophic, heterotrophic, batch sequential, nutrient conditions) to enhance metabolite production (Wan et al, 2015), as shown in (Chang et al, 2018;Chiang et al, 2020;Dambek et al, 2012;Erdogan et al, 2021;Fierli et al, 2022;Gao et al, 2020;Kim et al, 2010b;Lakey-Beitia et al, 2019;Li et al, 2000;Li et al, 2021;Masse et al, 2004;Okcu et al, 2021;Qin et al, 2013;Tachihana et al, 2020;Wei et al, 2022;Wu et al, 2011;Yang and Wei, 2020; Table 1. Compared to separate cultivation, the two-stage mode offers advantages such as high product yield, energy saving, environmental protection and a wide range of applications (Liyanaarachchi et al, 2021).…”

Section: Selection and Breeding Of Fucoxanthinproducing Microalgaementioning

confidence: 99%

Progress on the biological characteristics and physiological activities of fucoxanthin produced by marine microalgae

Gong,

Ma,

Yan

et al. 2024

Front. Mar. Sci.

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BackgroundFucoxanthin is a carotenoid found in seaweed. Its unique chemical structure gives it a variety of properties. Thus fucoxanthin have attracted the attention of companies and researchers.MethodsScientific papers were collected from the database. Duplicates and unavailable literature were excluded first. Then the remaining literature was categorized for referencing in the review.ResultsThis article contains a summary of the microalgae species producing fucoxanthin and their progress in breeding and cultivation modes. Additionally, the review summarized the progress of research on physiological activities and organized the experimental models used in these studies.ConclusionsThese present findings may provide information for the upstream production of fucoxanthin from algal species selection to process optimization. The analysis of the physiological activity results will help advance subsequent physiological and biochemical experiments. Furthermore, it intends to pique researchers’ enthusiasm for fucoxanthin and enrich related research data to accelerate the development of this natural product.

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Specific light-regime adaptations, terpenoid profiles and engineering potential in ecologically diversePhaeodactylum tricornutumstrains

Morelli,

Patwari,

Pruckner

et al. 2024

Preprint

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Microalgae, and among them, the diatomPhaeodactylum tricornutumstand out with their remarkable versatility and metabolic engineering potential. Diatoms exhibit substantial variability in metabolism, photosynthetic physiology and environmental adaptation, even across the same species. These factors can affect the design and outcome of metabolic engineering strategies. In this study, we profiled the diversity of biotechnologically relevant traits of threeP. tricornutumstrains (Pt1, Pt6, and Pt9) under different light regimes to identify the most suitable chassis to be employed as bio-factory to produce high-value terpenoids. We conducted detailed assessments of these strains, using pulse amplitude modulated (PAM) fluorometry to measure photosynthetic efficiency and we analyzed the composition of pigments and triterpenoids, as main terpenoid metabolic sinks. Parameters such as the maximum quantum yield of PSII (Fv/Fm), the efficiency of excitation energy capture (Fv’/Fm’), and OJIP kinetics were used to estimate photosynthetic performance in different light regimes. Additionally, we evaluated their transformation efficiency and their capacity to produce heterologous monoterpenoids, using geraniol as a model product. Our findings revealed that Pt1, widely used in laboratories, exhibits robust growth and photosynthetic performance under standard laboratory conditions. Pt6, adapted to intertidal environments, shows unique resilience in fluctuating conditions, while Pt9, with its high-temperature tolerance, excels under continuous high irradiance. Additionally, this variability across strains and light conditions influenced the metabolic output of each strain. We concluded that understanding the physiological responses of differentP. tricornutumstrains to light is crucial for optimizing their use in metabolic engineering. The insights gained from this research will facilitate the strategic selection and exploitation of these strains in algae biotechnology, enhancing the production of commercially valuable compounds such as high-value terpenoids and derivatives. This comprehensive characterization of strains under varying light conditions offers a pathway to more efficient and targeted metabolic engineering applications.HighlightsPt1, Pt6, and Pt9 exhibit distinct physiology under different light regimes.Pt9 is photosynthetically more performant in continuous light, Pt6 in photoperiod.Light regimes affect pigments and triterpenoid content in all three strains.Each strain exhibits a specific carotenoid and triterpenoid composition.Bacterial conjugation of episomes varies across strains, it is more efficient in Pt1.Pt1 is suited for the heterologous synthesis of monoterpenes (geraniol).

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Exploring the potential of photosynthetic induction factor for the commercial production of fucoxanthin in Phaeodactylum tricornutum

Cited by 3 publications

References 31 publications

Mixotrophic cultivation of microalgae for carotenoid production

Mixotrophic cultivation of microalgae for carotenoid production

Progress on the biological characteristics and physiological activities of fucoxanthin produced by marine microalgae

Specific light-regime adaptations, terpenoid profiles and engineering potential in ecologically diversePhaeodactylum tricornutumstrains

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