Exploring the response of Actinobacteria to the presence of phosphorus salts sources: Metabolic and co‐metabolic processes

Abstract

liquid media containing Ca 3 PO 4 ; AlPO 4 or FePO 4 as the sole phosphate source, and discusses the possible mechanisms involved in this process. P solubilization by different strains was accompanied by a significant drop in pH from 7.0 to 2.15-5.0 after 14 days. The efficiency of different strains depended on the P-source. Streptomyces spp. MM140 and MM141 were the most efficient in solubilizing Ca 3 PO 4 , MM136, and MM141 were the most efficient in solubilizing AlPO 4 , while all strains were equally efficient in solubilizing FePO 4 . Gluconic, oxalic, citric, malic, succinic, formic, and acetic acid were detected in the medium with Ca 3 PO 4 , while all except acetic acid were detected in the media with FePO 4 or AlPO 4 . Although we did not use an organic source of phosphorus in the media, all strains produced acid and alkaline phosphatase. It is concluded from this study that actinobacteria produced multiple organic acids followed by a decrease in the pH to solubilize phosphate salts.As well as producing phosphatase, these microorganisms were found to have different ways of making P available, suggesting an ecological advantage as they form part of soil microbiomes important for plants. K E Y W O R D Sactinobacteria, phosphate solubilization, organic acids, Streptomyces

SenX3-RegX3, an Important Two-Component System, Regulates Strain Growth and Butenyl-spinosyn Biosynthesis in Saccharopolyspora pogona

“…The biosynthesis of natural products produced by most actinomycetes is affected by phosphate concentrations in the medium ( Romero-Rodríguez et al., 2018 ; Martín et al., 2012 ; Solans et al., 2019 ). Initially, the phosphate effect was attributed to a decrease or inhibition of natural product production as a result of a phosphate increase in the culture medium ( Curdová et al., 1976 ).…”

Diversity and function of culturable actinobacteria in the root-associated of Salvia miltiorrhiza Bunge

“…Nitrogen and phosphorus are indispensable nutrients for plant growth and development. There is evidence that the root-associated actinobacteria can increase plant nutrient utilization by fixing nitrogen and dissolving phosphorus, consequently promoting plant growth ( Anita & Richau, 2013 ; Solans et al, 2019 ). Iron is an essential trace mineral for almost all organisms, and it influences various activities and cell metabolisms, such as respiration and photosynthesis.…”

An Actinobacterium Strain From Soil of Cerrado Promotes Phosphorus Solubilization and Plant Growth in Soybean Plants

“…Previous studies have reported the liberation of phosphate by Actinobacteria, especially by Streptomyces species. Hamdali et al (2008) reported the capacity of several strains isolated from three Moroccan phosphate mining centers to solubilize phosphate in a synthetic minimum medium supplemented with rock phosphate; two had values of 21.43 and 29.67 µg L −1 after a 5 day incubation at 28 • C. Oliveira et al (2009) described six strains that belong to the Streptomyces genus In the same study, two Arthrobacter strains solubilized 28 and 30.5 mg phosphate L −1 sodium phytate after a 10 day incubation at 27 • C. Wang et al (2018) describes a strain of S. griseoplanus isolated from the maize rhizosphere that solubilized 284.80 µg phosphate ml −1 tricalcium phosphate after a 20 day incubation at 28 • C. Similarly, Solans et al (2019) evaluated five actinobacterial strains, two of which, belonging to the genus Streptomyces, were the most efficient solubilizers of Ca 3 PO 4 of 2.27 and 2.149 mg L −1 (PO4 −2 ) g −1 , respectively, after 14 days at 28 • C.…”

“…Prieto-Correal et al ( 2015) evaluated 15 isolates of Streptomyces, 14 of which were able to produce organic acids in both tricalcium phosphate and aluminum phosphate media, most frequently citric, tartaric, and gluconic acids. Solans et al (2019) evaluated five Streptomyces isolates, all of which were able to produce organic acids in both calcium phosphate and aluminum phosphate media, most frequently gluconic, oxalic, citric, and malic acids. In this study, we identified a S. rishiriensis isolate with the ability to solubilize P and identified that both gluconic and 2-ketogluconic acids were involved in the process.…”