This article examines the acceptance of aquaculture development by residents and visitors of two islands, Cephallonia and Ithaki, in the Ionian Sea, in western Greece. Aquaculture farms have been established recently in both islands, and processing and packaging units in Cephallonia, generating jobs for the local communities and land rents for the villages in the coastal areas near the fish cages. However, the farms and processing and packaging units entail certain negative impacts on the environment. This study reveals that aquaculture farms have found better acceptance in Ithaki, where aquaculture is a form of development suited to this remote, small, predominantly rural island. The degree of acceptability is higher for locals than for tourists, but also for islanders who do not live in the areas where the processing units are situated. Respondents who consider the pollution of the marine environment by the farms to be high, mostly locals and residents of the villages in the vicinity of the aquaculture infrastructure or the main cities in the islands, are more likely to express a negative attitude toward aquaculture and its further development.