Exploring the Synergy Between HPTLC and HPLC-DAD for the Investigation of Wine-Making By-Products

Abstract

Both environmental and economic issues are increasingly pushing for the revalorization of agri-food by-products, including those arising from wine industry. Wastes produced from wine-making processes are important sources of biologically active compounds, mainly phenolic acids and flavonoids, which could be re-used for several applications, for example as additive surrogates or new ingredients in foodstuffs and/or pharmaceuticals. Therefore, the development of methods aimed at isolating, characterizing and quantifying molecules present in winery by-products acquires considerable importance in view of their re-utilization on a large scale. In this connection, this study demonstrated that high-performance thin-layer chromatography (HPTLC) and high-performance liquid chromatography with diode array detection (HPLC-DAD) can operate in synergy for the investigation of pomace and seed materials arising from both white and red cultivars of Vitis Vinifera. By virtue of fingerprint profiling, mass spectrometry (MS) interfacing and band comparison method, HPTLC enabled detection and identification of phenolic acids, non-anthocyanic flavonoids and anthocyanins. On the contrary, only anthocyanins could be identified by HPLC-DAD, and their subsequent quantification showed that malvidin-3-O-glucoside (oenin) was the most abundant one. In parallel, HPTLC has allowed to detect and quantify proanthocyanidins (PAC), showing that only catechin was present in the test samples. Both quantitative analytical methods were validated in terms of linearity, detection and quantification limits and precision.

Compounds from Vernonia arborea Buch.-Ham. Inhibit Microbes that Impair Wound Healing

“…Wound microbes contribute to major impairment in healing process, altering the wound microenvironment by prolonging inflammation, inducing excessive exudate formation, digesting wound tissue with extracellular enzymes, exacerbating devascularization caused by the wound, impeding re-epithelialization [28,29]. Among the listed mechanisms, prolonged inflammation is the primary stumbling block that expedites the repertoire of the other challenging events.…”

Cyclooxygenase (COX) and 15-Lipoxygenase (15-LOX) Inhibitory Activity and HPTLC Profile of Asplenium Nidus, Diplazium Esculentum, and Drynaria Quercifolia in Bukidnon, Philippines

“…Various colors of bands at different Rf values may correspond to the different phytochemicals. According to Bernardi et al (2019), blue fluorescent and purple spots can be attributed to phenolic acids and anthocyanins, respectively while flavonoids appeared as orange or yellow spots. Chlorophylls are fluorescing bright red (Reich et al, 2007).…”

Enzymatic Conversion of Mannan-Rich Plant Waste Biomass into Prebiotic Mannooligosaccharides

“…The use of TLC has been adapted to more sophisticated techniques that allow for better quantification of oligosaccharides. These include high performance-TLC (HPTLC) which is a rapid and cost-effective method that offers a high sensitivity of analysis and produces reproducible results [ 65 ]. The use of HPTLC also requires the use of a small amount of sample, which means that MOS produced will not be wasted during their analysis [ 65 ].…”

“…These include high performance-TLC (HPTLC) which is a rapid and cost-effective method that offers a high sensitivity of analysis and produces reproducible results [ 65 ]. The use of HPTLC also requires the use of a small amount of sample, which means that MOS produced will not be wasted during their analysis [ 65 ]. HPTLC works on the same principle as normal TLC, having a solvent mobile phase that moves along the stationary phase due to capillary action, which helps the analytes that have a lower affinity for the stationary phase to move faster along the plate than those with a higher stationary phase affinity which move slower along with the plate.…”