Under the current promotion of the national strategy of connotative development and the quality improvement of higher education, local application-oriented institutions, as an important part of China's higher education system, have a direct relationship with their development status and the quality level to the overall quality of higher education and regional economic and social development. However, these colleges and universities are facing a series of problems such as unclear concept, imperfect system, and lack of practice path in the construction of quality culture, and the existence of these problems seriously restricts the enhancement of their education quality and school running level. Aiming at the above problems, this study adopts the methods of literature analysis, case study and comparative study to explore in depth the connotation and characteristics of quality culture in local application-oriented institutions, and systematically constructs the development path of construction of quality culture from the two dimensions of theory and practice.