Exploring the Therapeutic Potentials of Exopolysaccharides Derived From Lactic Acid Bacteria and Bifidobacteria: Antioxidant, Antitumor, and Periodontal Regeneration

Khalil, M. I.; Sonbol, Fatma Ibrahim; Al-Madboly, Lamiaa A.; Aboshady, Tamer A.; Alqurashi, Abeer S; Ali, Sameh S.

doi:10.3389/fmicb.2022.803688

Cited by 36 publications

(9 citation statements)

References 92 publications

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“…This was then followed by vigorous mixing for 10 min and overnight incubation at 37 °C. The growth of probiotic bacteria in DMEM has been well established in the literature for most Lactobacillus species and Bifidobacteria 67 , 68 . In addition, the bacterial growth was further confirmed by noting the turbidity and measuring the optical density (OD600nm = 0.94 ± 0.162).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of immunomodulatory potential of probiotic conditioned medium on murine macrophages

Al-Najjar,

Abdulrazzaq,

Alzaghari

et al. 2024

Sci Rep

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Probiotics are a mixture of beneficial live bacteria and/or yeasts that naturally exist in our bodies. Recently, numerous studies have focused on the immunostimulatory effects of single-species or killed multi-species probiotic conditioned mediums on macrophages. This study investigates the immunostimulatory effect of commercially available active, multi-species probiotic conditioned medium (CM) on RAW264.7 murine macrophages. The probiotic CM was prepared by culturing the commercially available probiotic in a cell-culture medium overnight at 37 °C, followed by centrifugation and filter-sterilization to be tested on macrophages. The immunostimulatory effect of different dilution percentages (50%, 75%, 100%) of CM was examined using the MTT assay, proinflammatory cytokine (tumor necrosis factor TNF-alpha) production in macrophages, migration, and phagocytosis assays. For all the examined CM ratios, the percentages of cell viability were > 80%. Regarding the migration scratch, TNF-alpha and phagocytosis assays, CM demonstrated a concentration-dependent immunostimulatory effect. However, the undiluted CM (100%) showed a significant (p-value < 0.05) stimulatory effect compared to the positive and negative controls. The findings suggest that the secretions and products of probiotics, as measured in the CM, may be closely associated with their immune-boosting effects. Understanding this relationship between probiotic secretions and immune function is crucial for further exploring the potential benefits of probiotics in enhancing overall health and well-being.

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Section: Methodsmentioning

confidence: 99%

Evaluation of immunomodulatory potential of probiotic conditioned medium on murine macrophages

Al-Najjar,

Abdulrazzaq,

Alzaghari

et al. 2024

Sci Rep

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“…Different types of EPS show different antioxidant abilities under conditions of oxidative stress. Among the antioxidant ability of EPS derived from LAB and Bifidobacteria , EPS5 performs best as a result of the chemical groups as hydroxyl group and high content of sugars as rhamnose and ribose when compared to others …”

Section: Structure–function Relationship Of the Antioxidant Ability O...mentioning

confidence: 99%

“…Among the antioxidant ability of EPS derived from LAB and Bif idobacteria, EPS5 performs best as a result of the chemical groups as hydroxyl group and high content of sugars as rhamnose and ribose when compared to others. 48 Molecular Weight. While molecular weight has a significant impact on the biological properties of the molecule, its contribution to the antioxidant ability of EPS is a matter of debate.…”

Section: ■ Structure−function Relationship Of the Antioxidant Ability...mentioning

confidence: 99%

Relationship of Gene-Structure–Antioxidant Ability of Exopolysaccharides Derived from Lactic Acid Bacteria: A Review

Zhou

Zeng

et al. 2023

J. Agric. Food Chem.

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Polysaccharides derived from lactic acid bacteria (LAB) have widespread industrial applications owing to their excellent safety profile and numerous biological properties. The antioxidant activity of exopolysaccharides (EPS) offers defense against disease conditions caused by oxidative stress. Several genes and gene clusters are involved in the biosynthesis of EPS and the determination of their structures, which play an important role in modulating their antioxidant ability. Under conditions of oxidative stress, EPS are involved in the activation of the nonenzyme (Keap1-Nrf2-ARE) response pathway and enzyme antioxidant system. The antioxidant activity of EPS is further enhanced by the targeted alteration of their structures, as well as by chemical methods. Enzymatic modification is the most commonly used method, though physical and biomolecular methods are also frequently used. A detailed summary of the biosynthetic processes, antioxidant mechanisms, and modifications of LAB-derived EPS is presented in this paper, and their gene-structure−function relationship has also been explored.

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“…The majority of studies have designated the dose- or/and time-dependent cytotoxic effect of EPSs on CRC cell lines in vitro ( Table 2 ) [ 153 , 154 , 155 , 156 , 157 , 158 , 159 , 160 ]. In several cases, EPSs trigger the intrinsic apoptotic pathway activation, indicated by the increased expression of Bax, caspase-3, and caspase-9 [ 161 , 162 ] and decreased levels of Bcl-2 [ 163 , 164 , 165 ]. EPSs from L. plantarum NCU116 activate the c-Jun dependent Fas/FasL-mediated apoptotic pathway through TLR2 in mouse intestinal epithelial cancer cells [ 166 ].…”

Section: Probiotic Derived Bioactive Compounds and Crcmentioning

confidence: 99%

Probiotic-Derived Bioactive Compounds in Colorectal Cancer Treatment

Thoda

Touraki

2023

Microorganisms

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Colorectal cancer (CRC) is a multifactorial disease with increased morbidity and mortality rates globally. Despite advanced chemotherapeutic approaches for the treatment of CRC, low survival rates due to the regular occurrence of drug resistance and deleterious side effects render the need for alternative anticancer agents imperative. Accumulating evidence supports that gut microbiota imbalance precedes the establishment of carcinogenesis, subsequently contributing to cancer progression and response to anticancer therapy. Manipulation of the gut microbiota composition via the administration of probiotic-derived bioactive compounds has gradually attained the interest of scientific communities as a novel therapeutic strategy for CRC. These compounds encompass miscellaneous metabolic secreted products of probiotics, including bacteriocins, short-chain fatty acids (SCFAs), lactate, exopolysaccharides (EPSs), biosurfactants, and bacterial peptides, with profound anti-inflammatory and antiproliferative properties. This review provides a classification of postbiotic types and a comprehensive summary of the current state of research on their biological role against CRC. It also describes how their intricate interaction with the gut microbiota regulates the proper function of the intestinal barrier, thus eliminating gut dysbiosis and CRC development. Finally, it discusses the future perspectives in precision-medicine approaches as well as the challenges of their synthesis and optimization of administration in clinical studies.

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Exploring the Therapeutic Potentials of Exopolysaccharides Derived From Lactic Acid Bacteria and Bifidobacteria: Antioxidant, Antitumor, and Periodontal Regeneration

Abstract: Graphical AbstractTherapeutic applications of exopolysaccharides derived from lactic acid bacteria and bifidobacteria.

Cited by 36 publications

References 92 publications

Evaluation of immunomodulatory potential of probiotic conditioned medium on murine macrophages

Evaluation of immunomodulatory potential of probiotic conditioned medium on murine macrophages

Relationship of Gene-Structure–Antioxidant Ability of Exopolysaccharides Derived from Lactic Acid Bacteria: A Review

Probiotic-Derived Bioactive Compounds in Colorectal Cancer Treatment

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