Virtual online laboratory simulations for subjects with laboratory component became a trend in universities during COVID-19 pandemic. This study determined the perception of the respondents on the use of Labster online virtual simulation (LVOS) in terms of benefits/opportunities, challenges/drawback, the suggestions of the respondents and the comparison between IPL and LVOS. Mix method (Descriptivequalitative and quantitative) using a questionnaire was used. Frequency, percentages, and T-test were used to analyze the data. Thirty-three BS Human Biology students for school year 2021-2022, were the respondents in this study. Findings revealed that laboratory classes during pandemic was continued using LVOS. Perceived opportunities of LVOS are; easy to learn, has almost real experimental illustrations, need no assistance, do not require laboratory base equipment, all experimental procedures can be learned, helpful tutorial mode in solving post experiment queries and, enhances analysis and reasoning. Perceived challenges are; absence of actual microscopy, preparation of slides from fresh specimen is lacking, require high version of computers and, skills in microscopy is not develop. In comparison LVOS is more advantageous in terms of, convenient to use, repetition of experiment enhanced mastery, better experimental procedures, more interesting, and time saving. IPL on the other hand promotes analysis of facts, more interaction between professor and students. It is suggested that LVOS should be a part of laboratory classes in life sciences.