Exploring the use of wild relatives in potato breeding through integrated cytogenetic and genomic approaches

Abstract

Papa te llamas papa y no patata, no naciste castellana: eres oscura como nuestra piel, somos americanos... Pablo Neruda ("Oda a la papa", 1954) Contents Chapter 1 General introduction 9 Chapter 2 Introgressive Hybridization in Potato Revealed by Novel Cytogenetic and Genomic Technologies 23 Chapter 3 Pairing analysis and in situ Hybridisation reveal autopolyploid-like behaviour in Solanum commersonii x S. tuberosum (potato) interspecific hybrids 51 Chapter 4 Collinearity between potato (Solanum tuberosum L.) and wild relatives assessed by comparative cytogenetic mapping 73 Chapter 5 Comparative analysis of repetitive sequences among species from the potato and the tomato clades 9 7 Chapter 6 Comparative genomics of potato (Solanum tuberosum L.) and the diploid wild relatives Solanum commersonii and S. chacoense 12 1 Chapter 7 Intact DNA purified from flow-sorted nuclei unlocks the potential of next-generation genome mapping and assembly in Solanum species 1 5 1 Chapter 8 General discussion 165 Summary-Samenvatting-Resumen 177 Acknowledgements 183 Curriculum Vitae 189 Publications 190 Education Statement of the Graduate School Experimental Plant Sciences 191 General Introduction Bradshaw JE (2007b) Breeding potato as a major staple crop. In: Kang M, Priyadarshan P (eds) Breeding major food staples.

Genetic Diversity and Population Structure of Potato Germplasm in RDA-Genebank: Utilization for Breeding and Conservation

“…Potato wild relatives could be used for various purposes in potato breeding program, such as disease resistance and environmental tolerance [12][13][14]. The efficient use of wild relatives now requires extensive knowledge of their allelic variation and genomic structure, including the screening for desirable traits [15].…”