Exploring Trichoderma and Aspergillus secretomes: Proteomics approaches for the identification of enzymes of biotechnological interest

Cologna, Nicholas de Mojana di; Gómez‐Mendoza, Diana Paola; Zanoelo, Fabiana Fonseca; Giannesi, Giovana Cristina; Guimarães, Nelciele Cavalieri de Alencar; Moreira, Leonora Rios de Souza; Filho, Edivaldo Ximenes Ferreira; Ricart, Carlos André Ornelas

doi:10.1016/j.enzmictec.2017.08.007

Cited by 52 publications

(24 citation statements)

References 111 publications

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“…Notably, among enzymes related to saccharification and liquefaction in J3, glucoamylases were clearly active with high expression levels, indicating their collaborative roles along with high temperature in degrading starches, which would be spontaneously decomposed under high temperature, as well as suggesting a feasible way to mine thermostable glucoamylases from J3. The majority of enzymes related to saccharification and liquefaction in J3 were highly expressed by fungal species of R. emersonii , A. oryzae , A. fumigatus , and C. immitis , some of which have been found to secrete numerous carbohydrate-active enzymes and show high capacities toward degrading polymers, such as Aspergillus (Culleton et al, 2013; de Vries et al, 2017; Cologna et al, 2018) and R. emersonii (Hua et al, 2014; Martínez et al, 2016). In addition, J3 showed considerable potential for converting glucose to pivotal intermediates, such as acetate, ethanol, pyruvate, and acetyl-coA, which might then serve as direct or indirect substrates for JF flavor compounds including ethyl acetate, ethyl butanoate, ethyl propanoate, ethyl 2-hydroxypropanoate, ethyl 2-hydroxyhexanoate, acetic acid, 2-acetylpyridine, hexyl acetate, benzyl acetate ethyl, ethyl 3-methylbutanoate, ethyl benzeneacetate, and 3-methylbutyl acetate (Fan et al, 2012; Wang et al, 2014; Xiao et al, 2016; Gao et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Unraveling the Contribution of High Temperature Stage to Jiang-Flavor Daqu, a Liquor Starter for Production of Chinese Jiang-Flavor Baijiu, With Special Reference to Metatranscriptomics

Yi¹,

Jin²,

Xiao³

et al. 2019

Front. Microbiol.

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Jiang-flavor (JF) daqu is a liquor starter used for production of JF baijiu, a well-known distilled liquor in China. Although a high temperature stage (70°C) is necessary for qualifying JF daqu, little is known regarding its active microbial community and functional enzymes, along with its role in generating flavor precursors for JF baijiu aroma. In this investigation, based on metatranscriptomics, fungi, such as Aspergillus and Penicillium, were identified as the most active microbial members and 230 carbohydrate-active enzymes were identified as potential saccharifying enzymes at 70°C of JF daqu. Notably, most of enzymes in identified carbohydrate and energy pathways showed lower expression levels at 70°C of JF daqu than those at the high temperature stage (62°C) of Nong-flavor (NF) daqu, indicating lowering capacities of saccharification and fermentation by high temperature stage. Moreover, many enzymes, especially those related to the degradation of aromatic compounds, were only detected with low expression levels at 70°C of JF daqu albeit not at 62°C of NF daqu, indicating enhancing capacities of generating special trace aroma compounds in JF daqu by high temperature stage. Additionally, most of enzymes related to those capacities were highly expressed at 70°C by fungal genus of Aspergillus, Coccidioides, Paracoccidioides, Penicillium, and Rasamsonia. Therefore, this study not only sheds light on the crucial functions of high temperature stage but also paves the way to improve the quality of JF baijiu and provide active community and functional enzymes for other fermentation industries.

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Section: Discussionmentioning

confidence: 99%

Unraveling the Contribution of High Temperature Stage to Jiang-Flavor Daqu, a Liquor Starter for Production of Chinese Jiang-Flavor Baijiu, With Special Reference to Metatranscriptomics

Yi¹,

Jin²,

Xiao³

et al. 2019

Front. Microbiol.

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“…They characterized by specificity, stability and extremely fast labor under moderate temperatures and pressures. So, they are considered safe and eco-friendly (Cologna et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Biological Control of Pectinophora Gossypiella (Saunders) Using Spores and Supernatants of some Entomopathogenic Fungi

Abd-ElAzeem¹,

El-Sayed

El-Gendy

2020

Journal of Plant Protection and Pathology

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The spores and supernatants of some fungal isolates; Paecilomyces violacea, Trichoderma harzianum and Beauveria bassiana; were evaluated to control the pink bollworm, Pectinophora gossypiella biologically at Plant Protection Research Institute, Sharkia branch, (ARC). All fungi were isolated from soil and dead pink bollworm larvae. B. bassiana was the most active isolate caused the greatest larval and pupal mortality, also the highest deformed adult, also, the lowest numbers of eggs and hatchability percentage. Protease and chitinase produced from B. bassiana. strain had high effects on percent of larval mortality, comparing with control. Identification of Beauvaria sp. was assured by using 18s rRNA with accession number MT644472.1.

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“…In the last few years, several advances have been achieved in different research fields and contributed to a better understanding of the physiology and key features behind the T. reesei hypercellulolytic capacity. Such advances include the development of new tools for genetic manipulation (Derntl et al, 2015 ; Liu et al, 2015 ), transcriptomic and proteomic studies using lignocellulosic residues (Dos Santos Castro et al, 2014 ; Borin et al, 2015 , 2017 ; Daly et al, 2017 ; Ellilä et al, 2017 ; Cologna et al, 2018 ), the discovery of new transcription factors (TFs) and regulatory elements (Derntl et al, 2016 , 2017 ; Stappler et al, 2017 ; Zheng et al, 2017b ; Benocci et al, 2018 ), promoter characterization (Zheng et al, 2017a ; Kiesenhofer et al, 2018 ) and structural studies of cellulases (Li et al, 2015 ; Bodenheimer and Meilleur, 2016 ; Eibinger et al, 2016 ; Ma et al, 2017 ; Borisova et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Gene Co-expression Network Reveals Potential New Genes Related to Sugarcane Bagasse Degradation in Trichoderma reesei RUT-30

Borin

Carazzolle

Santos

et al. 2018

Front. Bioeng. Biotechnol.

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The biomass-degrading fungus Trichoderma reesei has been considered a model for cellulose degradation, and it is the primary source of the industrial enzymatic cocktails used in second-generation (2G) ethanol production. However, although various studies and advances have been conducted to understand the cellulolytic system and the transcriptional regulation of T. reesei, the whole set of genes related to lignocellulose degradation has not been completely elucidated. In this study, we inferred a weighted gene co-expression network analysis based on the transcriptome dataset of the T. reesei RUT-C30 strain aiming to identify new target genes involved in sugarcane bagasse breakdown. In total, ~70% of all the differentially expressed genes were found in 28 highly connected gene modules. Several cellulases, sugar transporters, and hypothetical proteins coding genes upregulated in bagasse were grouped into the same modules. Among them, a single module contained the most representative core of cellulolytic enzymes (cellobiohydrolase, endoglucanase, β-glucosidase, and lytic polysaccharide monooxygenase). In addition, functional analysis using Gene Ontology (GO) revealed various classes of hydrolytic activity, cellulase activity, carbohydrate binding and cation:sugar symporter activity enriched in these modules. Several modules also showed GO enrichment for transcription factor activity, indicating the presence of transcriptional regulators along with the genes involved in cellulose breakdown and sugar transport as well as other genes encoding proteins with unknown functions. Highly connected genes (hubs) were also identified within each module, such as predicted transcription factors and genes encoding hypothetical proteins. In addition, various hubs contained at least one DNA binding site for the master activator Xyr1 according to our in silico analysis. The prediction of Xyr1 binding sites and the co-expression with genes encoding carbohydrate active enzymes and sugar transporters suggest a putative role of these hubs in bagasse cell wall deconstruction. Our results demonstrate a vast range of new promising targets that merit additional studies to improve the cellulolytic potential of T. reesei strains and to decrease the production costs of 2G ethanol.

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Exploring Trichoderma and Aspergillus secretomes: Proteomics approaches for the identification of enzymes of biotechnological interest

Cited by 52 publications

References 111 publications

Unraveling the Contribution of High Temperature Stage to Jiang-Flavor Daqu, a Liquor Starter for Production of Chinese Jiang-Flavor Baijiu, With Special Reference to Metatranscriptomics

Unraveling the Contribution of High Temperature Stage to Jiang-Flavor Daqu, a Liquor Starter for Production of Chinese Jiang-Flavor Baijiu, With Special Reference to Metatranscriptomics

Biological Control of Pectinophora Gossypiella (Saunders) Using Spores and Supernatants of some Entomopathogenic Fungi

Gene Co-expression Network Reveals Potential New Genes Related to Sugarcane Bagasse Degradation in Trichoderma reesei RUT-30

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