Exploring vivo toxicity assessment of copper oxide nanoparticle in Wistar rats

Mohammadyari, Alireza; Razavipour, Seyedeh Tahereh; Mohammadbeigi, Maryam; Negahdary, Masoud; Ajdary, Marziyeh

doi:10.15412/j.jbtw.01030601

Cited by 19 publications

(17 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, reducing the dosage of dietary copper (both CuS and CuNP) increased the activity of plasma AST, ALT and ALP. Given the opposite data presented by numerous authors [ 29 , 44 , 45 ] the results of our study were surprising and difficult to interpret, especially as the values of some of the parameters in the CuD (Cu-deficient) group were similar to those obtained in the high-dosage treatment while the values of others were similar to those noted in the low-dosage treatment. Similarly, Shukla et al [ 46 ], administering 1 mg Cu/kg BW in the form of CuSO 4 to Wistar rats orally for 16 weeks observed a significant decrease in ALT activity in both the blood and the liver, but no effect was on AST activity.…”

Section: Discussioncontrasting

confidence: 99%

“…Lee et al [ 26 ] stressed that copper nanoparticles, having a much larger specific surface area than macro- or microparticles, can cause completely different biological reactions. Mohammadyari et al [ 45 ] administered CuO nanoparticles (50 nm) to Wistar rats by intraperitoneal injection at dosages of 0, 100, 200 or 400 ppm for 15 d and observed a significant increase in serum AST, ALT and ALP activity only in the rats from the 400 ppm CuO-nano treatment as compared to 0 ppm. In a study in which rat diets were supplemented with CuNPs or CuMPs at doses of 100, 200 or 400 mg Cu/kg BW per d, AST, ALT, ALP and LDH activity increased with the dose of Cu [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Comparison of the effect of dietary copper nanoparticles and one copper (II) salt on the copper biodistribution and gastrointestinal and hepatic morphology and function in a rat model

et al. 2018

View full text Add to dashboard Cite

The aim of the study was to investigate the effect of two forms (CuCO3 (CuS); and Cu nanoparticles (CuNP)) and dosages (standard 6.5 mg/kg (H), half of the standard (L)) of additional dietary Cu administered to growing rats on gastrointestinal and hepatic function and morphology. Copper in the form of CuNP vs CuS caused lower Cu faecal/urinal excretion and increased Cu accumulation in the brain tissue. Hepatic high-grade hydropic degeneration and necrotic lesions were observed only in the CuNP-H animals. In the lower gut, the dietary application of CuNP stifled bacterial enzymatic activity of caecal gut microbiota and resulted in lower SCFA production. That diminishing effect of CuNP on caecal microbiota activity was accompanied by a relative increase in the secretion of glycoside hydrolases by bacterial cells. The results showed that in comparison to Cu from CuCO3, Cu nanoparticles to a greater extent were absorbed from the intestine, accumulated in brain tissue, exerted antimicrobial effect in the caecum, and at higher dietary dose caused damages in the liver of rats.

show abstract

Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Comparison of the effect of dietary copper nanoparticles and one copper (II) salt on the copper biodistribution and gastrointestinal and hepatic morphology and function in a rat model

et al. 2018

View full text Add to dashboard Cite

show abstract

“…According to our results reported in Table 1 , we observed a significant elevation in ALT and AST enzyme activity and TB level, the efficient indicators for liver damage, in group II. In the liver injury, the transport function of hepatocytes is disturbed, resulting in leakage of plasma membrane and elevation of the serum level of liver enzymes [ 28 , 29 ]. The increased TB concentration might be attributed to the failure of normal uptake, conjugation and excretion by the damaged hepatic parenchyma [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

Ameliorative Influence of Green Tea Extract on Copper Nanoparticle-Induced Hepatotoxicity in Rats

Ibrahim¹,

Khalaf²,

Galal³

et al. 2015

Nanoscale Res Lett

View full text Add to dashboard Cite

The potential toxicity of copper nanoparticles (CNPs) to the human health and environment remains a critical issue. In the present study, we investigated the protective influence of an aqueous extract of green tea leaves (GTE) against CNPs-induced (20–30 nm) hepatotoxicity. Four different groups of rats were used: group I was the control, group II received CNPs (40 mg/kg BW), group III received CNPs plus GTE, and group IV received GTE alone. We highlighted the hepatoprotective effect of GTE against CNPs toxicity through monitoring the alteration of liver enzyme activity, antioxidant defense mechanism, histopathological alterations, and DNA damage evaluation. The rats that were given CNPs only had a highly significant elevation in liver enzymes, alteration in oxidant-antioxidant balance, and severe pathological changes. In addition, we detected a significant elevation of DNA fragmentation percentage, marked DNA laddering, and significance over expression of both caspase-3 and Bax proteins. The findings for group III clarify the efficacy of GTE as a hepatoprotectant on CNPs through improving the liver enzyme activity, antioxidant status, as well as suppressing DNA fragmentation and the expression of the caspase-3 and Bax proteins. In conclusion, GTE was proved to be a potential hepatoprotective additive as it significantly ameliorates the hepatotoxicity and apoptosis induced by CNPs.

show abstract

“…Many reports verify substantial toxicity of CuO NPs, induced damages in nephrotic and hepatic tissues of rats that affect physiology and biochemical functioning of kidney and liver [11]. Mohammadyari et al [99] found rise in ALT and ALP upon treatment of CuO NPs demonstrating disturbance in liver's membrane that lead to cellular release of these enzymes. Lee et al [100] observed dose‐dependent increase in AST, ALP, ALT, TBIL, CRE, BUN and LDH while the level of TP (total protein) and TG (triglyceride) decreased significantly.…”

Section: Biochemical Alterations Induced By Cuo Npsmentioning

confidence: 99%

Toxicity of copper oxide nanoparticles: a review study

Naz

Gul

Zia³

2019

IET nanobiotechnol.

258

View full text Add to dashboard Cite

Copper oxide nanoparticles (CuO NPs) use has exponentially increased in various applications (such as industrial catalyst, gas sensors, electronic materials, biomedicines, environmental remediation) due to their flexible properties, i.e. large surface area to volume ratio. These broad applications, however, have increased human exposure and thus the potential risk related to their short-and long-term toxicity. Their release in environment has drawn considerable attention which has become an eminent area of research and development. To understand the toxicological impact of CuO NPs, this review summarises the in-vitro and in-vivo toxicity of CuO NPs subjected to species (bacterial, algae, fish, rats, human cell lines) used for toxicological hazard assessment. The key factors that influence the toxicity of CuO NPs such as particle shape, size, surface functionalisation, time-dose interaction and animal and cell models are elaborated. The literature evidences that the CuO NPs exposure to the living systems results in reactive oxygen species generation, oxidative stress, inflammation, cytotoxicity, genotoxicity and immunotoxicity. However, the physio-chemical characteristics of CuO NPs, concentration, mode of exposure, animal model and assessment characteristics are the main perspectives that define toxicology of CuO NPs.

show abstract

Exploring vivo toxicity assessment of copper oxide nanoparticle in Wistar rats

Cited by 19 publications

References 17 publications

Comparison of the effect of dietary copper nanoparticles and one copper (II) salt on the copper biodistribution and gastrointestinal and hepatic morphology and function in a rat model

Comparison of the effect of dietary copper nanoparticles and one copper (II) salt on the copper biodistribution and gastrointestinal and hepatic morphology and function in a rat model

Ameliorative Influence of Green Tea Extract on Copper Nanoparticle-Induced Hepatotoxicity in Rats

Toxicity of copper oxide nanoparticles: a review study

Contact Info

Product

Resources

About