Exponential rolling circle amplification coupled with lateral flow dipstick strips as a rapid and sensitive method for the field detection of Karlodinium veneficum

Liu, Fu Guo; Chen, Guo Fu; Zhang, Chun Yun; Wang, Yuan Yuan; Zhou, Jin

doi:10.1007/s10811-019-01762-4

Cited by 20 publications

(6 citation statements)

References 49 publications

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“…Tests developed for aquaculture diseases using PCR/LFA include Vibrio harveyi (Thongkao, Longyant, Silprasit, Sithigorngul, & Chaivisuthangkura, 2015), red‐spotted grouper nervous necrosis virus (Su et al., 2015), white spot syndrome (Jaroenram, Kiatpathomchai, & Flegel, 2009), and the marine pathogen red sea bream iridovirus and viral hemorrhagic septicemia virus (Seo et al., 2020). Similarly, early identification of harmful bloom‐forming algal is critical for the management of marine ecosystems, marine fisheries, and public health and PCR/LFA assays have been developed for several algal species including Skeletonema costatum (Huang et al., 2017), Amphidinium carterae (Wang, Chen, Zhang, Wang, & Sun, 2019), and Karlodinium veneficum (Liu, Chen, Zhang, Wang, & Zhou, 2019).…”

Section: Discussionmentioning

confidence: 99%

Sensitive environmental DNA detection via lateral flow assay (dipstick)—A case study on corallivorous crown‐of‐thorns sea star (Acanthaster cf. solaris) detection

Doyle

Uthicke

2020

Environmental DNA

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Environmental DNA (eDNA) represents an emerging opportunity for species monitoring in the marine environment. One aspect that poses challenges is the ability to detect target DNA without the complexity of specialized laboratory equipment. Lateral flow is an analytical technique that has been adopted in point‐of‐care diagnostics for human, veterinary, and agricultural health. Here, we aim to use lateral flow assay as a detection method for eDNA monitoring using a commercially available nucleic acid lateral flow device (PCRD™) in combination with previously developed species‐specific mtDNA primers. Episodic population explosions of coral‐eating crown‐of‐thorns sea star (CoTS) contribute significantly to the coral reef crisis on tropical Pacific coral reefs. Laboratory testing revealed our lateral flow assay developed for CoTS was as sensitive as digital droplet PCR and able to detect < 10 copies of target DNA, per PCR. Furthermore, the lateral flow assay was completed in less than half the time compared with digital droplet PCR and cost less than half that of digital droplet PCR. We applied this method to eDNA water samples collected in field locations where CoTS were present in low (=nonoutbreak) population densities and found lateral flow assay to be sufficiently sensitive to detect these populations. Detection of low‐density CoTS populations is critical for early warning of outbreaks and leads to early management interventions (e.g., culling). Importantly, we demonstrate that with species‐specific primers, the development and application of lateral flow assay methods for eDNA detection are feasible, for example, for invasive or threatened species, or those with high conservation value.

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Section: Discussionmentioning

confidence: 99%

Sensitive environmental DNA detection via lateral flow assay (dipstick)—A case study on corallivorous crown‐of‐thorns sea star (Acanthaster cf. solaris) detection

Doyle

Uthicke

2020

Environmental DNA

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“…Product formation may also be measured through colorimetric means, using hydroxy naphthol blue (Hamidi and Ghourchian, 2015). Lateral flow technology can also be used to detect labelled, amplified products (Liu et al, 2019).…”

Section: Rolling Circle Amplificationmentioning

confidence: 99%

Virus Detection: A Review of the Current and Emerging Molecular and Immunological Methods

Cassedy

Parle‐McDermott

O’Kennedy

2021

Front. Mol. Biosci.

134

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Viruses are ubiquitous in the environment. While many impart no deleterious effects on their hosts, several are major pathogens. This risk of pathogenicity, alongside the fact that many viruses can rapidly mutate highlights the need for suitable, rapid diagnostic measures. This review provides a critical analysis of widely used methods and examines their advantages and limitations. Currently, nucleic-acid detection and immunoassay methods are among the most popular means for quickly identifying viral infection directly from source. Nucleic acid-based detection generally offers high sensitivity, but can be time-consuming, costly, and require trained staff. The use of isothermal-based amplification systems for detection could aid in the reduction of results turnaround and equipment-associated costs, making them appealing for point-of-use applications, or when high volume/fast turnaround testing is required. Alternatively, immunoassays offer robustness and reduced costs. Furthermore, some immunoassay formats, such as those using lateral-flow technology, can generate results very rapidly. However, immunoassays typically cannot achieve comparable sensitivity to nucleic acid-based detection methods. Alongside these methods, the application of next-generation sequencing can provide highly specific results. In addition, the ability to sequence large numbers of viral genomes would provide researchers with enhanced information and assist in tracing infections.

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“…Compared with the results obtained by Kor et al [ 44 ], the sensitivity of miRNA21 was enhanced 7.5 times. Moreover, RCA integrated with LFA were designed for the detection of Karenia mikimotoi (hyperbranched RCA, HRCA [ 45 ]) and Karlodinium veneficum (exponential RCA, E-RCA [ 46 ]), respectively. Inspiringly, the sensitivity of HRCA-LFA was 100 times that of polymerase chain reaction (PCR) [ 45 ], and E-RCA-LFA was more sensitive than that of the conventional PCR and reached a LOD of 0.01 cell/mL [ 46 ].…”

Section: Sensitivity Enhancement Based On Sample Pretreatmentmentioning

confidence: 99%

“…Moreover, RCA integrated with LFA were designed for the detection of Karenia mikimotoi (hyperbranched RCA, HRCA [ 45 ]) and Karlodinium veneficum (exponential RCA, E-RCA [ 46 ]), respectively. Inspiringly, the sensitivity of HRCA-LFA was 100 times that of polymerase chain reaction (PCR) [ 45 ], and E-RCA-LFA was more sensitive than that of the conventional PCR and reached a LOD of 0.01 cell/mL [ 46 ]. In addition, RCA-based LFA has also been used in the detection of metal ions [ 47 ], small molecules [ 48 ], enzymes [ 49 ], and antibodies [ 50 ].…”

Section: Sensitivity Enhancement Based On Sample Pretreatmentmentioning

confidence: 99%

Recent advances in sensitivity enhancement for lateral flow assay

Deng

Jiang

et al. 2021

Microchim Acta

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Conventional lateral flow assay (LFA) is typically performed by observing the color changes in the test lines by naked eyes, which achieves considerable commercial success and has a significant impact on the fields of food safety, environment monitoring, disease diagnosis, and other applications. However, this qualitative detection method is not very suitable for low levels of disease biomarkers’ detection. Although many nanomaterials are used as new labels for LFA, additional readers limit their application to some extent. Fortunately, a lot of work has been done for improving the sensitivity of LFA. In this review, currently reported LFA sensitivity enhancement methods with an objective evaluation are summarized, such as sample pretreatment, the change of flow rate, and label evolution, and future development direction and challenges of LFAs are discussed.

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Exponential rolling circle amplification coupled with lateral flow dipstick strips as a rapid and sensitive method for the field detection of Karlodinium veneficum

Cited by 20 publications

References 49 publications

Sensitive environmental DNA detection via lateral flow assay (dipstick)—A case study on corallivorous crown‐of‐thorns sea star (Acanthaster cf. solaris) detection

Sensitive environmental DNA detection via lateral flow assay (dipstick)—A case study on corallivorous crown‐of‐thorns sea star (Acanthaster cf. solaris) detection

Virus Detection: A Review of the Current and Emerging Molecular and Immunological Methods

Recent advances in sensitivity enhancement for lateral flow assay

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