During the last three decades, nightlife policies in Southern European cities have been directed towards promoting the night as a space–time for tourism-oriented promotion. At the same time, highly precarious, often racialised migrant actors performing informal activities during the night have been (re-)criminalised, put under surveillance and persecuted by public discourse and policy-making. The Covid-19 pandemic has revealed the centrality of ‘the night’ as a fundamental cornerstone for urban governance. However, analysis of how debates on urban nightlife dialogue with frameworks on urban in/formality, security and governance during the day require a more systematic analysis. In this commentary, we call into question the role of the in/formal urban night in ordering neoliberal cities in Southern Europe. By focussing on informal workers during the night as exemplar cases of how in/formal nocturnal governance is produced, we propose an approach to incorporate deeper explorations in future nightlife studies along three avenues: (i) contradictory public discourses encompassed by ‘the night’, and how they are affected by long-term cultural, neo-colonial legacies and ‘darkness’ archetypes; (ii) survival and resistance strategies conducted by precarious/subaltern nocturnal actors during the day and night; and (iii) urban governance arrangements shaping and being shaped by the in/formal night in contemporary ‘Fortress Europe’. The research agenda suggested in this critical commentary aims to be a provocation, not only for nightlife scholars, but also for broader urban studies to take into deeper consideration how the criminalisation of ‘In/formal Nocturnal Cities’ is used in governance processes in contemporary (post-)pandemic cities.