Exposure of a photosystem II complex to chemically generated singlet oxygen results in D1 fragments similar to the ones observed during aerobic photoinhibition

Mishra, Neelam; Ghanotakis, Demetrios F.

doi:10.1016/0005-2728(94)90003-5

Cited by 46 publications

(30 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As one ROS species, 1 O 2 is primarily produced by a reaction of an oxygen molecule with the triplet P 680 inside the PSII RC (Kirilovsky et al 1990). It has been reported that the 1 O 2 could initiate chlorophyll bleaching, protein degradation, and crosslinking (Setlik et al 1990;Vass et al 1992;Mishra and Ghanotakis 1994). We further found that quinone protects the PSII RC complex from photodamage, and more interestingly, the protection is associated with the generation of superoxide anion (O 2 -Á ), another well-known ROS species (Liu et al 2001;Liu et al 2004).…”

mentioning

confidence: 61%

Damage to the oxygen-evolving complex by superoxide anion, hydrogen peroxide, and hydroxyl radical in photoinhibition of photosystem II

Liu

Wang

et al. 2006

Photosynth Res

View full text Add to dashboard Cite

Under strong illumination of a photosystem II (PSII) membrane, endogenous superoxide anion, hydrogen peroxide, and hydroxyl radical were successively produced. These compounds then cooperatively resulted in a release of manganese from the oxygen-evolving complex (OEC) and an inhibition of oxygen evolution activity. The OEC inactivation was initiated by an acceptor-side generated superoxide anion, and hydrogen peroxide was most probably responsible for the transportation of reactive oxygen species (ROS) across the PSII membrane from the acceptor-side to the donor-side. Besides ROS being generated in the acceptor-side induced manganese loss; there may also be a ROS-independent manganese loss in the OEC of PSII. Both superoxide anion and hydroxyl radical located inside the PSII membrane were directly identified by a spin trapping-electron spin resonance (ESR) method in combination with a lipophilic spin trap, 5-(diethoxyphosphoryl)-5-phenethyl-1-pyrroline N-oxide (DEPPEPO). The endogenous hydrogen peroxide production was examined by oxidation of thiobenzamide.

show abstract

mentioning

confidence: 61%

Damage to the oxygen-evolving complex by superoxide anion, hydrogen peroxide, and hydroxyl radical in photoinhibition of photosystem II

Liu

Wang

et al. 2006

Photosynth Res

View full text Add to dashboard Cite

show abstract

“…We found that incubation of the 160 kD complex in gel slices under aerobic conditions brings about fragmentation of the complex, probably as a result of oxidative effects on the D 1 and D2 proteins (Mishra and Ghanotakis 1994). This fragmentation helped to expose antigenic epitopes of D2, as evidenced by the strong cross reaction between D2 antibodies and an ,-~80 kD piece (Fig.…”

Section: Resultsmentioning

confidence: 92%

Characterization of a 160 kD Photosystem II reaction center complex isolated from photoinhibited Dunaliella salina thylakoids

Melis

Nemson

1995

Photosynth Res

View full text Add to dashboard Cite

Photoinhibition in the green alga Dunaliella salina is accompanied by the formation of inactive Photosystem II reaction centers. In SDS-PAGE analysis, the latter appear as 160 kD complexes. These complexes are structurally stable, enough to withstand re-electrophoresis of excised gel slices from the 160 kD region. Western blot analyses with specific polyclonal antibodies raised against the D1 or D2 reaction center proteins provided evidence for the presence of both of these polypeptides in the re-electrophoresed 160 kD complex. Incubation of excised gel slices from the 160 kD region, under aerobic conditions at 4°C for a prolonged period of time, caused a break-up of the 160 kD complex into a ∽52 kD D1-containing and ∽80 and ∽26 kD D2-containing pieces. Western blot analysis with polyclonal antibodies raised against the apoproteins of CPI (reaction center proteins of PS I) did not show cross-reaction either with the 160 kD complex or with the ∽52, ∽80 and ∽26 kD pieces. The results show the presence of both D1 and D2 in the 160 kD complex and strengthen the notion of a higher molecular weight D1- and D2-containing complex that forms upon disassembly of photodamaged PS II units.

show abstract

“…This drop in F m is well characterised since it is due to a proteolytic degradation of the damaged D1 protein (Aro et al 1993). The small recovery of F m observed for Epace 10 at 30 min may suggest a repair of the PS2 RCs via de novo synthesis of D1 protein and reassembly of the functional PS2 RCs (Mishra and Ghanotakis 1994). This situation probably does not occur in the other genotypes, because PAR continues to reach the damaged PS2 complex and proteins other than D1, may be D2 (Schuster et al 1988), CP43 and CP47 (Nedbal et al 1990), and may ensue in irreversible loss of PS2 electron transport.…”

Section: Resultsmentioning

confidence: 92%

“…Although ROS may operate as a secondary messenger involved in signalling transduction in response to abiotic stress (Mittler 2002), high concentrations of ROS are highly harmful to the cell integrity and function (Wise and Naylor 1987a,b) causing lipid peroxidation at the membrane level (Choudhury and Behera 2001). Consequently, the ability of plants to maintain their membrane integrity under high irradiance (HI) stress determines their resistance capacity, and can be used as a criterion for stress resistance evaluation (Lauriano et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Short-term responses of photosynthetic membrane lipids and photochemical efficiency in plants of Phaseolus vulgaris and Vigna unguiculata submitted to high irradiance

Ferreira¹,

Bressan-Smith²,

Elias³

et al. 2007

Photosynt.

View full text Add to dashboard Cite

Primary leaves of young plants of common bean (Phaseolus vulgaris cv. Carioca and Negro Huasteco) and cowpea (Vigna unguiculata Walp cv. Epace 10) were exposed to high irradiance (HI) of 2 000 µmol m -2 s -1 for 10, 20, and 30 min. The initial fluorescence (F 0 ) was nearly constant in response to HI in each genotype except for Carioca. A distinct reduction of maximum fluorescence (F m ) was clearly observed in stressed genotypes of beans after 20 min followed by a slight recovery for the longer stress times. In common bean, the maximum quantum yield (F v /F m ) was reduced slowly from 10 to 30 min of HI. In cowpea, only a slight reduction of F v /F m was observed at 20 min followed by recovery to normal values at 30 min. HI resulted in changes in the photochemical (q P ) and non-photochemical (q N ) quenching in both species, but to a different extent. In cowpea plants, more efficiency in the use of the absorbed energy under photoinhibitory conditions was related to increase in q P and decrease in q N . In addition, lipid peroxidation changed significantly in common bean genotypes with an evident increase after 20 min of HI. Hence the photosynthetic apparatus of cowpea was more tolerant to HI than that of common bean and the integrity of cowpea cell membranes was apparently maintained under HI.

show abstract

Exposure of a photosystem II complex to chemically generated singlet oxygen results in D1 fragments similar to the ones observed during aerobic photoinhibition

Cited by 46 publications

References 28 publications

Damage to the oxygen-evolving complex by superoxide anion, hydrogen peroxide, and hydroxyl radical in photoinhibition of photosystem II

Damage to the oxygen-evolving complex by superoxide anion, hydrogen peroxide, and hydroxyl radical in photoinhibition of photosystem II

Characterization of a 160 kD Photosystem II reaction center complex isolated from photoinhibited Dunaliella salina thylakoids

Short-term responses of photosynthetic membrane lipids and photochemical efficiency in plants of Phaseolus vulgaris and Vigna unguiculata submitted to high irradiance

Contact Info

Product

Resources

About